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Schuler, T.J., Hutcheson, O.S. and Downey, RK. 1992. Heterosis in intervarietal hybrids of summer tumip rape in western Canada. Canadian Joumal of Plant Science. 72: 1 27-1 36, Schuster, W. 1982 Need of hetemsis 8-s by synthetic varieties of Bmsska napus and Sinapis alba. Vortrage Manzenzüchtung. 1 : 137-156. Senaratna, Tm, Kott, Lm, Beversdorf, W.D. and McKersie, 8.D. 1991. Dessication of microspore derived embryos of oilseed rape (Bmsska napus L). Plant Cell Reports. 10:342-344. Smith, S. and Chin, E. 1992. The utility of random primermediateci profiles. RFLP's, and other technologies to provide useful data for varietal protection. In: Joint Plant Breeding Symposia Series: Applications of RAPO Technology to Plant Breeding. p 4649. Snape, J.W., Wright, AJ. and Simpson, E. 1984. Methods for estimating gene numbers for quantitative charaders using doubled haploid lines. Theoretical and Applied Genetics. 67: 1 43-1 48. Song, K. and Osbom, T.C. 1992. Polyphyletic origins of Brassica napus: new evidence based on organelle and nuclear RFLP analyses. Genome. 35:992- 1001. Sree Ramulu, K., Verhoeven, H A and Dijkhuis, P. 1991. Mitotic blocking, micronucleation, and chromosome doubling by oryzalin, amiprophos-mehtyl, and colchicine in potato. Protoplasma. l60:6Wl. Stringham, G.R, Degenhardt, DmFm, Thiagarajah, MmR and Bansal, V.K. 1995. Quantum summer rape. Canadian Journal of Plant Science. 75:903-904. Sun, V.G. 1938. SelfgoIlination in rape. Joumal of the Arnerican Society of Agronomy. 30:760-761. Swanson, €.B., Hemgesell, MrnJrn, Arnoldo, M., Sippell, D.W. and Wong, RSwCm 1989. Microspore mutagenesis and seledion: Canola plants with field tolerance to the imidazolinones. Theoretical and Applied Genetics. 785250530. Talbert, L.E., Blake, NmKm, Chee, P.W., Blake, T.K. and Magyar, GaM. 1994. Evaluation of "sequence-tagged-site" PCR products as molecular markers in wheat. Theoretical and Applied Genetics. 87:789-794.
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Page 1 and 2:
The Application of Ooubled Haploid
Page 3 and 4:
COPYRIGET P1ERbIISSION PAGE A Thesu
Page 5 and 6:
111 application in 8. rapa cultivar
Page 7 and 8:
v TABLE OF CONTENTS ABSTWT ACKNOWLE
Page 9 and 10:
VI1 LIST OF TABLES TABLE Page EFI',
Page 11 and 12:
lx LlST OF FIGURES FIGURE Page Prod
Page 13 and 14:
1.2 performance. Production of DH p
Page 15 and 16:
History of Canola Produdion of Bras
Page 17 and 18:
Economic Importance and Distributio
Page 19 and 20:
2.5 genes involved in recognizhg an
Page 21 and 22:
2.7 There is potential for loss of
Page 23 and 24:
2.9 their stability under different
Page 25 and 26:
2.1 1 selected and selfed to create
Page 27 and 28:
2.1 3 production (Mathias and Wbbel
Page 29 and 30:
2.1 5 traits have low heritability.
Page 31 and 32:
2.17 opposite strands of template D
Page 33 and 34:
Figure 2.3. The AFLP procedure usin
Page 35 and 36:
2.21 non-specifïc arüfacts which
Page 37 and 38:
2.23 maintenance of DNA extradion p
Page 39 and 40:
Cornparison of Bud Pollination and
Page 41 and 42:
INTRODUCTION Production of doubled
Page 43 and 44:
Effkiency lndex = total number of s
Page 46 and 47:
Application of doubfeâ haploid dev
Page 48 and 49:
Brassita rapa represents approximat
Page 50 and 51: 4.5 and a daylnight temperature of
Page 52 and 53: 4-7 plants within each tent using a
Page 54 and 55: 4.9 Days to Flowanng Reward OH Iine
Page 56 and 57: 4-11 In Canan, Reward Cja and Cl,,
Page 58 and 59: 4.13 counterparts. The extra genera
Page 60 and 61: 4-15 composite population can eithe
Page 62: Table 4.2. Mean parameter values fo
Page 68 and 69: Table 4.8. Correlation of days to f
Page 72 and 73: Detedion of Genetic Variation in Bm
Page 74: INTRODUCTION The production of doub
Page 77 and 78: 5-6 represented one sample from C,
Page 79 and 80: 5-8 the percent of polymorphism pre
Page 81 and 82: 5-10 and C, and a similar level of
Page 83 and 84: Table 5.2. Polymorphic levels in Cl
Page 86 and 87: 5-15 Table 5.5. Proportion of polyr
Page 88 and 89: 6. GENERAL DlSCUSSlON AND CONCLUSIO
Page 90 and 91: 6-3 B. rapa OH Iines. Seleded DH Ii
Page 92 and 93: Abramson, RD. 1995. Thermostable DN
Page 94 and 95: Diers, B.W. and Osbom, T.C. 1994. G
Page 96 and 97: Jasieniuk, M., Brill&Babel, AL. and
Page 98 and 99: Narasimhulu, SBw and Chopra, U.L. 1
Page 102 and 103: Williams, J.G.K., HanMey, M.K., Raf
Page 104 and 105: Appendix 12. Data matrix of RAPO pr
Page 106 and 107: Apperidi 1. (-nueû) Month D~Y Temp
Page 108 and 109: Mean Max [mm)
Page 110 and 111: 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1
Page 112: Append'bc 4. FM measummmts fw param
Page 116 and 117: Locaüon Plant Id. RedTcate €MG D
Page 118 and 119: C24 C24 C2-4 C2-8 C2-8 C2-8 C2-8 c2
Page 120 and 121: Location Plant Id. Replicate €MG
Page 122 and 123: Locaüori Plant ld. Replicate EMG O
Page 124 and 125: Appendix 10. The mean performance o
Page 127: f i . I 1 t a I 1 I I t t c i r' !m
Page 130 and 131: Appendii 12. Data matrix of RAPO pm
Page 133: Appendix 13. @mtïnued) Primer Comp
Page 138: Appendk 14. (continuedl Primer Comp
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