The Application of Ooubled Haploid Plants to Population ... - MSpace

More documents

Recommendations

Info

$il\VOLVEMENT OF RETII\OIC ACID II{ - MSpace at the University of ...$

4-7 plants within each tent using a feather duster every other day. Two tent replicates were grown for each of DSC-3 and Reward DH populations. The position of the plants in the pollination tent was rotated every 4 days after flowering began. Seeâ produced was harvested from single plants and an equal amount ftom each plant was compositeci to produce the first generation composite (C,). From each of these C, populations, 60 plants were seeâed in individual pots and randomly intemossed to produce the second generation composite (C,). The C, was pmduœd fiom 60 individual C, plants to ensure adequate seed production for the 1996 field season. All Cl and C, populations were grown aï two field locations in 1996 as separate Cl and C, RCB with 6 and 4 replications in Winnipeg and Caman respedively. The original DH lines with sufficient seed were evaluated in Winnipeg in two replications. In Winnipeg 3-m rows were planted with 0.3 g of seed and in Carman 5-m rows were planted with 0.5 g of seed with 60- spacing between each row. Reward check rows were planted to alternate with the OH lines, C, or C, plant rows. These check rows were used in the data analysis in the calculation of the Reward donor population mean. There was no donor information for the DSC-3 breeding line. Agronomie performance of Co, C, and C, populations was evaluated by measuring number of plants emerged per metre of row, days to flowering, days to maturity, height at maturity, lodging, seed yield, harvest index (HI) and seed oil content (Appendix 4-9). Plant rows representing DH lines and replicates of Cl and C, were hand harvested, tied and stooked in the field. Each plant row was weighed when dry (rnoisture content was not detemined) and threshed in the field when dry using a
4.8 stationary thresher. Seed yield was detemineci by weighing seed harvested from each plant row. HI was calwlatad as the seed yield per total biomass yield measured for each row. Seed oïl content was rneasured for each row from 20 g of seed using a Nuclear Magnetic Resonance spectrometer (Robertson et al., 1979). Data was analyzed through nearest neighbour analysis of variance (NNA) using Agr0basef4~ (Mulitre, 1992) statistical program. A standard error (SE) value was calwlated for each parameter using a wmplete data set. RESULTS Seedling Emergence In 1996. the Reward DH lines showed a lower mean level of emergence than the Reward donor population (Table 4.1 ; Appendix 1 Oa). DSCJ OH lines also expressed a low level of seedling emergence (Table 4.2). Poor emergence could be attnbuted to the poor seed quality obtained through seif-pdlination of the individual DH Iines that expressed inbreeding depression. lndividual Reward and WC3 OH lines showed variation in the level of seedling emergence (Table 4.3). There was no difference in seedling emergenœ between any of the population sizes in the Reward C, or between the C, and the donor population (Table 4.4). The Reward C, populations al1 had higher numbers of seedlings emerging than the Reward donor population (Table 4.5). There were no differences between any of the DSCJ C, and C, populations in seedling emergence (Table 4.6 and Table 4.7).
Page 1 and 2: The Application of Ooubled Haploid
Page 3 and 4: COPYRIGET P1ERbIISSION PAGE A Thesu
Page 5 and 6: 111 application in 8. rapa cultivar
Page 7 and 8: v TABLE OF CONTENTS ABSTWT ACKNOWLE
Page 9 and 10: VI1 LIST OF TABLES TABLE Page EFI',
Page 11 and 12: lx LlST OF FIGURES FIGURE Page Prod
Page 13 and 14: 1.2 performance. Production of DH p
Page 15 and 16: History of Canola Produdion of Bras
Page 17 and 18: Economic Importance and Distributio
Page 19 and 20: 2.5 genes involved in recognizhg an
Page 21 and 22: 2.7 There is potential for loss of
Page 23 and 24: 2.9 their stability under different
Page 25 and 26: 2.1 1 selected and selfed to create
Page 27 and 28: 2.1 3 production (Mathias and Wbbel
Page 29 and 30: 2.1 5 traits have low heritability.
Page 31 and 32: 2.17 opposite strands of template D
Page 33 and 34: Figure 2.3. The AFLP procedure usin
Page 35 and 36: 2.21 non-specifïc arüfacts which
Page 37 and 38: 2.23 maintenance of DNA extradion p
Page 39 and 40: Cornparison of Bud Pollination and
Page 41 and 42: INTRODUCTION Production of doubled
Page 43 and 44: Effkiency lndex = total number of s
Page 46 and 47: Application of doubfeâ haploid dev
Page 48 and 49: Brassita rapa represents approximat
Page 50 and 51: 4.5 and a daylnight temperature of
Page 54 and 55: 4.9 Days to Flowanng Reward OH Iine
Page 56 and 57: 4-11 In Canan, Reward Cja and Cl,,
Page 58 and 59: 4.13 counterparts. The extra genera
Page 60 and 61: 4-15 composite population can eithe
Page 62: Table 4.2. Mean parameter values fo
Page 68 and 69: Table 4.8. Correlation of days to f
Page 72 and 73: Detedion of Genetic Variation in Bm
Page 74: INTRODUCTION The production of doub
Page 77 and 78: 5-6 represented one sample from C,
Page 79 and 80: 5-8 the percent of polymorphism pre
Page 81 and 82: 5-10 and C, and a similar level of
Page 83 and 84: Table 5.2. Polymorphic levels in Cl
Page 86 and 87: 5-15 Table 5.5. Proportion of polyr
Page 88 and 89: 6. GENERAL DlSCUSSlON AND CONCLUSIO
Page 90 and 91: 6-3 B. rapa OH Iines. Seleded DH Ii
Page 92 and 93: Abramson, RD. 1995. Thermostable DN
Page 94 and 95: Diers, B.W. and Osbom, T.C. 1994. G
Page 96 and 97: Jasieniuk, M., Brill&Babel, AL. and
Page 98 and 99: Narasimhulu, SBw and Chopra, U.L. 1
Page 100 and 101: Schuler, T.J., Hutcheson, O.S. and
Page 102 and 103:
Williams, J.G.K., HanMey, M.K., Raf
Page 104 and 105:
Appendix 12. Data matrix of RAPO pr
Page 106 and 107:
Apperidi 1. (-nueû) Month D~Y Temp
Page 108 and 109:
Mean Max [mm)
Page 110 and 111:
2 3 4 5 6 7 8 9 10 11 12 13 14 15 1
Page 112:
Append'bc 4. FM measummmts fw param
Page 116 and 117:
Locaüon Plant Id. RedTcate €MG D
Page 118 and 119:
C24 C24 C2-4 C2-8 C2-8 C2-8 C2-8 c2
Page 120 and 121:
Location Plant Id. Replicate €MG
Page 122 and 123:
Locaüori Plant ld. Replicate EMG O
Page 124 and 125:
Appendix 10. The mean performance o
Page 127:
f i . I 1 t a I 1 I I t t c i r' !m
Page 130 and 131:
Appendii 12. Data matrix of RAPO pm
Page 133:
Appendix 13. @mtïnued) Primer Comp
Page 138:
Appendk 14. (continuedl Primer Comp
show all

The Application of Ooubled Haploid Plants to Population ... - MSpace

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?