The Application of Ooubled Haploid Plants to Population ... - MSpace

More documents

Recommendations

Info

$il\VOLVEMENT OF RETII\OIC ACID II{ - MSpace at the University of ...$

3.4 B. napus by causing the collapse of the callose (Fu et al., 1992). The purpose of this study was to detemine the effactiveness of NaCl solutions as cornpared to bud pollination in overcoming selfincompatibility in B. rapa. MATERIALS AND METHODS B. rapa cv Reward plants were studied under field conditions in 1995. The experiment was seeded on May 29 with 100 seeds per row. Plant rows were 3 m long and spaced 1 m apat The mean daylnight temperature frorn seeding to maturity was 2711 5°C. Pollination treatments were applied to four randomly selected rows. Each row represented a replication. Within each row five plants were selected for each treatment. Treatments included bud pollination, a 3% wlv NaCl solution and a 5% wlv NaCl solution. NaCl solutions of 3% and 5% were prepared with filtered water and then sprayed on the stigma and stamen of newly opened flowers using a mist bottle. Polyethylene bags were placed over individual plants. Spray treatments took place every 3 to 4 d during the flowering pefiod. Bud pollination was also applied. This involved emasculating buds approximately 2 to 3 days prior to opening and then pollinating the stigma with pollen from the same plant. The duration of treatment application and seed set were recorded. The eficiency index (EFI), compatibility index (CI) and economic index (El) were then calculated for eacii treatment as follows:
Effkiency lndex = total number of seeds set no. of minutes of treatment Cornpatibility Index = total no- of seeds set . total no. of flowers pollinated Economic lndex (%) = no. of seeds cmduœd mr treated dant no. of seeds produced per bud pollinated plant Treatrnent results from the four replicates were averaged and then analyzed using a means cornparison test with P>0.1. RESULTS AND DISCUSSION The EFI of both NaCl solutions was significantly higher than the €FI of bud pollination (Table 3.1 ). The NaCl treatments produced approximately equal €FI values. The CI of both NaCl treatrnents and the bud pollination treatment did not differ significantly (Table 3.1). All treatments promoted a similar level of self-oornpatibility. The El of both NaCl treatments was not significantly different from the bud pol lination treatment (Table 3.1 ). Approximately equal nurnbers of plants were required to produce the same amount of seed when using bud pollination or NaCl solution treatments. All treatments overcame self-incornpatibility and promoted self-pollination to a similar degree. The NaCl solution treatments were efficient, producing more seed with less time (EFI). Bud pollination is a time consuming, labour intensive process that is not any more effective at achieving seed set than NaCl solution treatments. This study
Page 1 and 2: The Application of Ooubled Haploid
Page 3 and 4: COPYRIGET P1ERbIISSION PAGE A Thesu
Page 5 and 6: 111 application in 8. rapa cultivar
Page 7 and 8: v TABLE OF CONTENTS ABSTWT ACKNOWLE
Page 9 and 10: VI1 LIST OF TABLES TABLE Page EFI',
Page 11 and 12: lx LlST OF FIGURES FIGURE Page Prod
Page 13 and 14: 1.2 performance. Production of DH p
Page 15 and 16: History of Canola Produdion of Bras
Page 17 and 18: Economic Importance and Distributio
Page 19 and 20: 2.5 genes involved in recognizhg an
Page 21 and 22: 2.7 There is potential for loss of
Page 23 and 24: 2.9 their stability under different
Page 25 and 26: 2.1 1 selected and selfed to create
Page 27 and 28: 2.1 3 production (Mathias and Wbbel
Page 29 and 30: 2.1 5 traits have low heritability.
Page 31 and 32: 2.17 opposite strands of template D
Page 33 and 34: Figure 2.3. The AFLP procedure usin
Page 35 and 36: 2.21 non-specifïc arüfacts which
Page 37 and 38: 2.23 maintenance of DNA extradion p
Page 39 and 40: Cornparison of Bud Pollination and
Page 41: INTRODUCTION Production of doubled
Page 46 and 47: Application of doubfeâ haploid dev
Page 48 and 49: Brassita rapa represents approximat
Page 50 and 51: 4.5 and a daylnight temperature of
Page 52 and 53: 4-7 plants within each tent using a
Page 54 and 55: 4.9 Days to Flowanng Reward OH Iine
Page 56 and 57: 4-11 In Canan, Reward Cja and Cl,,
Page 58 and 59: 4.13 counterparts. The extra genera
Page 60 and 61: 4-15 composite population can eithe
Page 62: Table 4.2. Mean parameter values fo
Page 68 and 69: Table 4.8. Correlation of days to f
Page 72 and 73: Detedion of Genetic Variation in Bm
Page 74: INTRODUCTION The production of doub
Page 77 and 78: 5-6 represented one sample from C,
Page 79 and 80: 5-8 the percent of polymorphism pre
Page 81 and 82: 5-10 and C, and a similar level of
Page 83 and 84: Table 5.2. Polymorphic levels in Cl
Page 86 and 87: 5-15 Table 5.5. Proportion of polyr
Page 88 and 89: 6. GENERAL DlSCUSSlON AND CONCLUSIO
Page 90 and 91: 6-3 B. rapa OH Iines. Seleded DH Ii
Page 92 and 93:
Abramson, RD. 1995. Thermostable DN
Page 94 and 95:
Diers, B.W. and Osbom, T.C. 1994. G
Page 96 and 97:
Jasieniuk, M., Brill&Babel, AL. and
Page 98 and 99:
Narasimhulu, SBw and Chopra, U.L. 1
Page 100 and 101:
Schuler, T.J., Hutcheson, O.S. and
Page 102 and 103:
Williams, J.G.K., HanMey, M.K., Raf
Page 104 and 105:
Appendix 12. Data matrix of RAPO pr
Page 106 and 107:
Apperidi 1. (-nueû) Month D~Y Temp
Page 108 and 109:
Mean Max [mm)
Page 110 and 111:
2 3 4 5 6 7 8 9 10 11 12 13 14 15 1
Page 112:
Append'bc 4. FM measummmts fw param
Page 116 and 117:
Locaüon Plant Id. RedTcate €MG D
Page 118 and 119:
C24 C24 C2-4 C2-8 C2-8 C2-8 C2-8 c2
Page 120 and 121:
Location Plant Id. Replicate €MG
Page 122 and 123:
Locaüori Plant ld. Replicate EMG O
Page 124 and 125:
Appendix 10. The mean performance o
Page 127:
f i . I 1 t a I 1 I I t t c i r' !m
Page 130 and 131:
Appendii 12. Data matrix of RAPO pm
Page 133:
Appendix 13. @mtïnued) Primer Comp
Page 138:
Appendk 14. (continuedl Primer Comp
show all

The Application of Ooubled Haploid Plants to Population ... - MSpace

Create successful ePaper yourself

Delete template?

Save as template?