The Application of Ooubled Haploid Plants to Population ... - MSpace

5-8
the percent of polymorphism present, which equals the nurnber of bands that were
polymorphic divided by the total number of bands. Primer 318 was not cansidered
because it produced only monornorphic bands in the DSC-3 DH Iines.
The number of individuals analyzed in C, and C, was deteminecl by the
frequency of bands expressd in the OH lines (Table 5.3). The lowest band frequency
(p) with a specific primer was calailated by dïviding the number of indhiduals with the
least frequently expressed band by the total number of individual Iines in the DH
population. Then the number of plants (N) sampled fmm C, and C, was calculated to
give a 95% probability (P) that the least frequent allele (p) would be represented, as
shown in the following equation (Jasieniuk et al., 1996):
RAPD fragment sizes were estimated ftom the gels in relation to the size marker.
Data matrix tables were compiied where bands were recorded as either present (1) or
absent (O), as show in Table 5.4 and Appendix 11-14.
RESULTS AND DISCUSSION
RA PD Analysis
Polyrnorphism was detected for 9 of the original 30 primers tested between the
DSC-3 and Reward donor populations (data not shown). When tested on the DH lines
in both populations, 5 prime- produced bands that expressed adequate intensity to