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4.13 counterparts. The extra generation of outuossing may be beneficial by increasing heterogeneity in these populations. This was supported by RAPD analysis that showed more genetic variation present in CZ4 and C, than in Cl, and C,, populations, respectively (Friesen, 1997). However, this was not apparent in the RAPD analysis of C, and C,, or with the other agronomic parameters that did not Vary significantly between C, and Ci populations. Therefore inaeased heterogeneity apparently contributes to improved performance of the C, populations but may not be the sole cause, The DH lines involved in the development of the C, populations were selected based on the phenotype of the individual DH lines and not on combining abilities expressed in progeny of controlled crosses. C, and C, yields therefore may not represent the potential yield attainable if cornbining ability was tested before interpollination to identify the optimum combination of DH lines. Another useful method of identifying desirable OH lines through heterogeneity is DNA analysis. This has been used to individually characterize B. napus cultivars (Mailer et al. 1994) and identiï genetic diversity in DH lines of B. rapa (Friesen, 1997) in the fom of RAPD analysis. RAPD analysis is a quick method of characterizing plant genotypes and measuring the potential for hybrid vigour to be expressed when they are intercrossed. The Reward composite populations took longer to reach first flower than the original Reward donor population but the days to maturity were not different. Therefore, the period of seed filling was reduced. Thurling (1991) demonstrated the
4.14 importance of the time between anthesis and mahirity by intmducing genes for early flowefing into a B. napus line and a 8. rapa population. Thurling (1991) concluded that the earlier flowefing resulted in a longer period of dry matter accumulation and greater seed yields. The later Rowering did not adversely affêct the performance of the composite populations in this study with the exception of the Reward Ct4 which was later flowering in Cannan. There was a positive correlation between days to Rowering and seed yield for the Reward C, population in Carman (Table 4.8). A negative correlation between days to flowering and seed yield was found with the Reward and DSC-3 DH lines and DSC-3 C, and C, populations in Cannan (Table 4.8). CONCLUSION The production of composite populations from DH lines has the potential of speeding up cultivar devalopment. As a breeding tool, irnprovements can be made in B. rapa populations by selecting OH lines with fixed traits followed by randorn interpollination to recover pertomance. This method avoids the short comings of traditional breeding systems such as the time required for repeated generations of inbreeding and selection seen with mass selection, the difficulty in producing a reliable pollination control system for hybrid production and the maintenance of original entries for reconstitution of a population as a synthetic. The individual DH lines do not exceed donor population performance but, when crossed to establish a composite, population performance can be recovered. This
Page 1 and 2:
The Application of Ooubled Haploid
Page 3 and 4:
COPYRIGET P1ERbIISSION PAGE A Thesu
Page 5 and 6:
111 application in 8. rapa cultivar
Page 7 and 8: v TABLE OF CONTENTS ABSTWT ACKNOWLE
Page 9 and 10: VI1 LIST OF TABLES TABLE Page EFI',
Page 11 and 12: lx LlST OF FIGURES FIGURE Page Prod
Page 13 and 14: 1.2 performance. Production of DH p
Page 15 and 16: History of Canola Produdion of Bras
Page 17 and 18: Economic Importance and Distributio
Page 19 and 20: 2.5 genes involved in recognizhg an
Page 21 and 22: 2.7 There is potential for loss of
Page 23 and 24: 2.9 their stability under different
Page 25 and 26: 2.1 1 selected and selfed to create
Page 27 and 28: 2.1 3 production (Mathias and Wbbel
Page 29 and 30: 2.1 5 traits have low heritability.
Page 31 and 32: 2.17 opposite strands of template D
Page 33 and 34: Figure 2.3. The AFLP procedure usin
Page 35 and 36: 2.21 non-specifïc arüfacts which
Page 37 and 38: 2.23 maintenance of DNA extradion p
Page 39 and 40: Cornparison of Bud Pollination and
Page 41 and 42: INTRODUCTION Production of doubled
Page 43 and 44: Effkiency lndex = total number of s
Page 46 and 47: Application of doubfeâ haploid dev
Page 48 and 49: Brassita rapa represents approximat
Page 50 and 51: 4.5 and a daylnight temperature of
Page 52 and 53: 4-7 plants within each tent using a
Page 54 and 55: 4.9 Days to Flowanng Reward OH Iine
Page 56 and 57: 4-11 In Canan, Reward Cja and Cl,,
Page 60 and 61: 4-15 composite population can eithe
Page 62: Table 4.2. Mean parameter values fo
Page 68 and 69: Table 4.8. Correlation of days to f
Page 72 and 73: Detedion of Genetic Variation in Bm
Page 74: INTRODUCTION The production of doub
Page 77 and 78: 5-6 represented one sample from C,
Page 79 and 80: 5-8 the percent of polymorphism pre
Page 81 and 82: 5-10 and C, and a similar level of
Page 83 and 84: Table 5.2. Polymorphic levels in Cl
Page 86 and 87: 5-15 Table 5.5. Proportion of polyr
Page 88 and 89: 6. GENERAL DlSCUSSlON AND CONCLUSIO
Page 90 and 91: 6-3 B. rapa OH Iines. Seleded DH Ii
Page 92 and 93: Abramson, RD. 1995. Thermostable DN
Page 94 and 95: Diers, B.W. and Osbom, T.C. 1994. G
Page 96 and 97: Jasieniuk, M., Brill&Babel, AL. and
Page 98 and 99: Narasimhulu, SBw and Chopra, U.L. 1
Page 100 and 101: Schuler, T.J., Hutcheson, O.S. and
Page 102 and 103: Williams, J.G.K., HanMey, M.K., Raf
Page 104 and 105: Appendix 12. Data matrix of RAPO pr
Page 106 and 107: Apperidi 1. (-nueû) Month D~Y Temp
Page 108 and 109:
Mean Max [mm)
Page 110 and 111:
2 3 4 5 6 7 8 9 10 11 12 13 14 15 1
Page 112:
Append'bc 4. FM measummmts fw param
Page 116 and 117:
Locaüon Plant Id. RedTcate €MG D
Page 118 and 119:
C24 C24 C2-4 C2-8 C2-8 C2-8 C2-8 c2
Page 120 and 121:
Location Plant Id. Replicate €MG
Page 122 and 123:
Locaüori Plant ld. Replicate EMG O
Page 124 and 125:
Appendix 10. The mean performance o
Page 127:
f i . I 1 t a I 1 I I t t c i r' !m
Page 130 and 131:
Appendii 12. Data matrix of RAPO pm
Page 133:
Appendix 13. @mtïnued) Primer Comp
Page 138:
Appendk 14. (continuedl Primer Comp
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