The Application of Ooubled Haploid Plants to Population ... - MSpace

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plants within each tent using a feather duster every other day. Two tent replicates were
grown for each of DSC-3 and Reward DH populations. The position of the plants in the
pollination tent was rotated every 4 days after flowering began. Seeâ produced was
harvested from single plants and an equal amount ftom each plant was compositeci to
produce the first generation composite (C,). From each of these C, populations, 60
plants were seeâed in individual pots and randomly intemossed to produce the second
generation composite (C,). The C, was pmduœd fiom 60 individual C, plants to
ensure adequate seed production for the 1996 field season.
All Cl and C, populations were grown aï two field locations in 1996 as separate
Cl and C, RCB with 6 and 4 replications in Winnipeg and Caman respedively. The
original DH lines with sufficient seed were evaluated in Winnipeg in two replications. In
Winnipeg 3-m rows were planted with 0.3 g of seed and in Carman 5-m rows were
planted with 0.5 g of seed with 60-
spacing between each row. Reward check rows
were planted to alternate with the OH lines, C, or C, plant rows. These check rows
were used in the data analysis in the calculation of the Reward donor population mean.
There was no donor information for the DSC-3 breeding line.
Agronomie performance of Co, C, and C, populations was evaluated by
measuring number of plants emerged per metre of row, days to flowering, days to
maturity, height at maturity, lodging, seed yield, harvest index (HI) and seed oil content
(Appendix 4-9). Plant rows representing DH lines and replicates of Cl and C, were
hand harvested, tied and stooked in the field. Each plant row was weighed when dry
(rnoisture content was not detemined) and threshed in the field when dry using a