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VI 3. EFFECT OF NaCl SPRAY ON SELF-INCOMPATIBILIlY Abstract ................................................................................................ Introduction ................................................................................................ Materials and Methods ........................................................................... ResuIts and Oiscussion ........................................................................... 4. APPLICATION OF DOUBLED HAPLOIDS IN 8. raps Abstract Introduction ................................................................................................ Materials and Methods ........................................................................... Results ................................................................................................ Discussion ................................................................................................ Conclusion ................................................................................................ 5. DETECTION OF GENETIC VARIATION USlNG RAPDs Abstract .......................................................................................... Introduction ................. . ,.-a .................................................................... Materials and Methods ...................................................*....................... Results and Discussion ........................................................................... 6. GENERAL DISCUSSION AND CONCLUSION 7. LITERATURE ClTEO 8. APPENDIX
VI1 LIST OF TABLES TABLE Page EFI', Cl2 and EP and seeds produœd after bud pollination, 3% and 5% NaCl solution treatment ................................................................ Mean parameter values for OH lines (Co) produced from 8. rapa cv. Reward and cornparkm to rnean values of the check Reward donor population in Winnipeg, 1 996 ........-....--....................-.....- Mean parameter values for DH lines (Co) produced from B. rapa breeding line DSC-3 and cornparison to mean values of the check Reward donor population in Winnipeg, 1996 ................................ 4-17 Range parameter values for OH lines produced from B. rapa cv. Reward and breeding line DSC-3, 1996 ..................................................... 4.18 Mean values for parameters characterizing the cv. Reward donor and C, populations produced from 4, 8, 12 or 22 DH lines of B. rapa cv. Reward, 1996. ........................................................................... Mean values for parameters charadefizing the cv. Reward donor and C, populations produced from 4,8, 12 or 22 DH lines of B. rapa cv. Reward, 1996. ........................................................................... 4.20 Mean values for parameters characterizing the cv. Reward donor and Cl populations pmduced from 4, 8, 12 or 22 DH lines of the B. rapa breedina line DSC-3. 1996, ..................................................... 4.21
Page 1 and 2: The Application of Ooubled Haploid
Page 3 and 4: COPYRIGET P1ERbIISSION PAGE A Thesu
Page 5 and 6: 111 application in 8. rapa cultivar
Page 7: v TABLE OF CONTENTS ABSTWT ACKNOWLE
Page 11 and 12: lx LlST OF FIGURES FIGURE Page Prod
Page 13 and 14: 1.2 performance. Production of DH p
Page 15 and 16: History of Canola Produdion of Bras
Page 17 and 18: Economic Importance and Distributio
Page 19 and 20: 2.5 genes involved in recognizhg an
Page 21 and 22: 2.7 There is potential for loss of
Page 23 and 24: 2.9 their stability under different
Page 25 and 26: 2.1 1 selected and selfed to create
Page 27 and 28: 2.1 3 production (Mathias and Wbbel
Page 29 and 30: 2.1 5 traits have low heritability.
Page 31 and 32: 2.17 opposite strands of template D
Page 33 and 34: Figure 2.3. The AFLP procedure usin
Page 35 and 36: 2.21 non-specifïc arüfacts which
Page 37 and 38: 2.23 maintenance of DNA extradion p
Page 39 and 40: Cornparison of Bud Pollination and
Page 41 and 42: INTRODUCTION Production of doubled
Page 43 and 44: Effkiency lndex = total number of s
Page 46 and 47: Application of doubfeâ haploid dev
Page 48 and 49: Brassita rapa represents approximat
Page 50 and 51: 4.5 and a daylnight temperature of
Page 52 and 53: 4-7 plants within each tent using a
Page 54 and 55: 4.9 Days to Flowanng Reward OH Iine
Page 56 and 57: 4-11 In Canan, Reward Cja and Cl,,
Page 58 and 59:
4.13 counterparts. The extra genera
Page 60 and 61:
4-15 composite population can eithe
Page 62:
Table 4.2. Mean parameter values fo
Page 68 and 69:
Table 4.8. Correlation of days to f
Page 72 and 73:
Detedion of Genetic Variation in Bm
Page 74:
INTRODUCTION The production of doub
Page 77 and 78:
5-6 represented one sample from C,
Page 79 and 80:
5-8 the percent of polymorphism pre
Page 81 and 82:
5-10 and C, and a similar level of
Page 83 and 84:
Table 5.2. Polymorphic levels in Cl
Page 86 and 87:
5-15 Table 5.5. Proportion of polyr
Page 88 and 89:
6. GENERAL DlSCUSSlON AND CONCLUSIO
Page 90 and 91:
6-3 B. rapa OH Iines. Seleded DH Ii
Page 92 and 93:
Abramson, RD. 1995. Thermostable DN
Page 94 and 95:
Diers, B.W. and Osbom, T.C. 1994. G
Page 96 and 97:
Jasieniuk, M., Brill&Babel, AL. and
Page 98 and 99:
Narasimhulu, SBw and Chopra, U.L. 1
Page 100 and 101:
Schuler, T.J., Hutcheson, O.S. and
Page 102 and 103:
Williams, J.G.K., HanMey, M.K., Raf
Page 104 and 105:
Appendix 12. Data matrix of RAPO pr
Page 106 and 107:
Apperidi 1. (-nueû) Month D~Y Temp
Page 108 and 109:
Mean Max [mm)
Page 110 and 111:
2 3 4 5 6 7 8 9 10 11 12 13 14 15 1
Page 112:
Append'bc 4. FM measummmts fw param
Page 116 and 117:
Locaüon Plant Id. RedTcate €MG D
Page 118 and 119:
C24 C24 C2-4 C2-8 C2-8 C2-8 C2-8 c2
Page 120 and 121:
Location Plant Id. Replicate €MG
Page 122 and 123:
Locaüori Plant ld. Replicate EMG O
Page 124 and 125:
Appendix 10. The mean performance o
Page 127:
f i . I 1 t a I 1 I I t t c i r' !m
Page 130 and 131:
Appendii 12. Data matrix of RAPO pm
Page 133:
Appendix 13. @mtïnued) Primer Comp
Page 138:
Appendk 14. (continuedl Primer Comp
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