manual of methods of analysis of foods - Ministry of Health and ...
manual of methods of analysis of foods - Ministry of Health and ...
manual of methods of analysis of foods - Ministry of Health and ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
METALS<br />
µg <strong>of</strong> Sn present in test solution X Dilution factor<br />
Tin content <strong>of</strong> sample = -----------------------------------------------------------<br />
mgm/ kg<br />
Weight in grams <strong>of</strong> sample taken<br />
Preparation <strong>of</strong> St<strong>and</strong>ard Curve:<br />
Pipette 0, 1, 2, 3, 4 <strong>and</strong> 5 ml <strong>of</strong> the Tin working st<strong>and</strong>ard solution (n –<br />
ii) into six separatory funnels <strong>and</strong> add sufficient 4.5M sulphuric acid<br />
solution to bring the total volume to 50 ml. Follow the steps 2 to 9 under<br />
“Determination” <strong>and</strong> note the absorbance <strong>of</strong> each st<strong>and</strong>ard.<br />
Calculate the linear expression (Y = a + bX) relating the Tin content <strong>of</strong><br />
the st<strong>and</strong>ard (X) to the measured absorbance (Y). Plot the calibration graph<br />
corresponding to the calculated linear regression.<br />
Note: Whenever the absorbance measured for the blank/st<strong>and</strong>ard zero<br />
exceed 0.070, reagent solutions should be renewed.<br />
(Ref:- Manual Methods <strong>of</strong> Analysis for Adulterants <strong>and</strong> Contaminants in<br />
Foods, I.C.M.R 1990 Page 160)<br />
65
Change language