LIFE09200604007 Tabish - Homi Bhabha National Institute

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Results Fig. 8: Frequency of second primary cancers in study subjects (n=24). Values in parenthesis denote the actual number out of 24 subjects in each category. All patients had both cancers in UADT except 4 patients who had one cancer in UADT and the second cancer in lung, breast or acute myeloid leukaemia. All UADT cancers were histologically squamous cell carcinomas. Various regions in buccal mucosa dominated in first primary cancer (42%) as well as site of second primary cancer (50%). Tongue (21%) and larynx (21%) were also frequent sites of first primary cancer while cancer of hard palate (13%) and tongue (9%) were next most frequent cancers as second primaries (Fig. 7, Fig. 8). Detailed demographics of MPN patients accrued for the study is described in Table 5. Blood sample from healthy controls that were cancer free, and were habit and age matched, were collected mainly from Advanced Centre for Treatment Research and Education in Cancer (ACTREC), while two samples were taken from healthy subjects belonging to Shendurjane Village, Wai, Satra, India. While collecting sample subjects were analysed with respect to gender, age, location, habit and family history of cancer, based on questionnaire similar to cases. Detailed demographic of control samples is described in Table 6. 86
Results Table 5: Demographics of MPN patients LCL Type Age Sex Habit I Site Cancer II Site E195* MPN 48 F Areca nut chewing Breast Hard palate E242* MPN 45 M Pan+Smoking+Tobacco chewing+Alcohol Tongue Hard palate E252* MPN 38 M Tobacco chewing Buccal mucosa Buccal mucosa E253* MPN 65 F Tobacco chewing Buccal mucosa Alveolus E254* MPN 71 F Nil Tongue Oesophagus E264* MPN 71 M Tobacco chewing Lip Mouth E265* MPN 48 M Nil Larynx Tonsil E270* MPN 60 M Tobacco chewing Alveolus Tongue E305 MPN 64 F Pan+Tobacco chewing RMT Upper alveolus E306 MPN 55 M Smoking+Pan+Tobacco chewing Larynx Hard palate E307 MPN 49 M Smoking Larynx Nasal coolumella E308 MPN 53 M Smoking Tongue Tongue E311 MPN 63 F Nil E319 MPN 59 M Smoking+Alcohol Lat. Border tongue Supraglottic Larynx Buccal mucosa Lung+ Oesophagus E320 MPN 45 F Pan+Tobacco chewing Right Cheek Left Cheek E321 MPN 49 F Tobacco chewing Left tongue Left bronchus E322 MPN 50 M Smoking Buccal mucosa Lower alveolus E325 MPN 43 F Tobacco chewing Buccal mucosa Lower alveolus E326 MPN 75 M Smoking Supraglottis Oesophagus E329 MPN 47 M Pan+Tobacco chewing GBS Lower alveolus+ buccal mucosa E330 MPN 63 F Nil Breast Floor of mouth E331 MPN 32 M E332 MPN 51 M Pan+Smoking+Tobacco chewing+Alcohol Pan+Smoking+Tobacco chewing+Alcohol AML Lung E333 MPN 55 F Pan+ Tobacco chewing Buccal mucosa Alveolus Parapharyngeal carcinoma GBS+RMT+ lower alveolus Key: * cell lines prepared by Ashwin Kotnis, MPN-multiple primary neoplasms, RMT-Retromolar Trigone, GBS- Gingivobuccal sulcus, AML-Acute myeloid leukaemia 87
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Genotype-Molecular Phenotype Correl
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CONTENTS Page No Synopsis 1 List of
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Synopsis
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Synopsis Genotype-Molecular Phenoty
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Synopsis Materials and Methods: 1.
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Synopsis loading control. PCR produ
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Synopsis 7.1 Percent cell death aft
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Synopsis slides were then dried and
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Synopsis range 41.77% - 90.95% at 5
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Synopsis 5. Genotyping of genes: Ge
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Synopsis G2/M arrest and delaying e
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Synopsis may have an important bear
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Synopsis References: 1. Bobba, R.;
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Abbreviations MOPS : 3-(N-morpholin
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List of Figures Fig. 28: Percent re
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Chapter 1 Introduction
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Introduction In previous studies fr
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Introduction important carcinogenes
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Review of Literature The yet undeci
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Review of Literature Fig. 2: Incide
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Review of Literature sustained angi
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Review of Literature 2.6 Genotype p
Page 49 and 50: Review of Literature 2.7.2 Biologic
Page 51 and 52: Review of Literature Table 1: Steps
Page 53 and 54: Review of Literature future analysi
Page 55 and 56: Review of Literature Inefficient ce
Page 57 and 58: Review of Literature XRCC1 (X-ray r
Page 59 and 60: Review of Literature 2.9.2 Gene inv
Page 61 and 62: Review of Literature MPO gene polym
Page 63 and 64: Review of Literature important role
Page 65 and 66: Materials and Methods Source of che
Page 67 and 68: Materials and Methods Method: EBV-t
Page 69 and 70: Materials and Methods Immunophenoty
Page 71 and 72: Materials and Methods with 500 μl
Page 73 and 74: Materials and Methods cDNA it can b
Page 75 and 76: Materials and Methods Cobalt-60 iso
Page 77 and 78: Materials and Methods specific bind
Page 79 and 80: Materials and Methods h half of the
Page 81 and 82: Materials and Methods phenol and th
Page 83 and 84: Materials and Methods 7. Filter ste
Page 85 and 86: Materials and Methods viz. EXO-SAP
Page 88 and 89: Materials and Methods Table 2: List
Page 90 and 91: Materials and Methods SMAD6 AREG HM
Page 92 and 93: Materials and Methods 3.9 Effect of
Page 94 and 95: Materials and Methods fresh eppendo
Page 96 and 97: Materials and Methods Power SYBR Gr
Page 98 and 99: Results Objective 1 To generate EBV
Page 102 and 103: Results Table 6: Demographics of he
Page 104 and 105: Results Fig. 10: Cell population do
Page 106 and 107: Results Fig. 12: Cell surface marke
Page 108 and 109: Results 4.1.5 Expression of ATM gen
Page 110 and 111: Results Objective 2 To compare the
Page 112 and 113: Results Fig. 17: Standardization of
Page 114 and 115: Results Fig. 19: Standardisation of
Page 116 and 117: Results The cells were incubated fu
Page 118 and 119: Results 4.2.3.1 Percent G2 delay af
Page 120 and 121: Results 4.2.3.2 Effect of BPDE expo
Page 122 and 123: Results Fig. 29: Comparison of perc
Page 124 and 125: Results Fig. 31: Percent cell death
Page 126 and 127: Results normalization with baseline
Page 128 and 129: Results 4.2.5.2 Real time PCR valid
Page 130 and 131: Results homozygous wild-type, heter
Page 132 and 133: Results Fig. 36b: Electrophoresis o
Page 134 and 135: Results Fig. 37a: Representative el
Page 136 and 137: Results Table 13: Consolidated geno
Page 138 and 139: Results LCL Genes NAT2(1) NAT2(2) N
Page 140 and 141: Results Fig. 38: Distribution of Ge
Page 142 and 143: Results Fig. 39: Various combinatio
Page 144 and 145: Results Table 14: Genotype-phenotyp
Page 146 and 147: Discussion Introduction Squamous ce
Page 148 and 149: Discussion stimulated lymphocytes w
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Discussion H2AX is currently the mo
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Discussion or G2/M phase arrest. As
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Discussion differential expression
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Discussion DNA repair genes and MPN
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Chapter 6 Summary and Conclusions
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Summary and Conclusions Statistical
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Bibliography 1. Dikshit, R.; Gupta,
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Bibliography 43. Lei, D.; Sturgis,
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Bibliography 76. Fry, R. C.; Svenss
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Bibliography 115. Kote-Jarai, Z.; M
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Bibliography 149. Hashibe, M.; Bren
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Bibliography 183. Bergamaschi, D.;
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Bibliography 216. Bondy, M. L.; Wan
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Indian J Med Res 135, June 2012, pp
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822 INDIAN J MED RES, JUNE 2012 mar
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824 INDIAN J MED RES, JUNE 2012 (h)
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826 INDIAN J MED RES, JUNE 2012 Tab
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828 INDIAN J MED RES, JUNE 2012 lik
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CASE REPORT Eben L. Rosenthal, MD,
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FIGURE 2. Chromosomal breakage anal
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FA along with ataxia telangiectasia
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