LIFE09200604007 Tabish - Homi Bhabha National Institute

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Bibliography 115. Kote-Jarai, Z.; Matthews, L.; Osorio, A.; Shanley, S.; Giddings, I.; Moreews, F.; Locke, I.; Evans, D. G.; Eccles, D.; Williams, R. D.; Girolami, M.; Campbell, C.; Eeles, R., Accurate prediction of BRCA1and BRCA2 heterozygous genotype using expression profiling after induced DNA damage. Clin Cancer Res 2006, 12, (13), 3896-901. 116. Thompson, L. H.; Brookman, K. W.; Jones, N. J.; Allen, S. A.; Carrano, A. V., Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange. Mol Cell Biol 1990, 10, (12), 6160-71. 117. Kowalski, M.; Przybylowska, K.; Rusin, P.; Olszewski, J.; Morawiec-Sztandera, A.; Bielecka- Kowalska, A.; Pietruszewska, W.; Mlynarski, W.; Janusz, S.; Majsterek, I., Genetic polymorphisms in DNA base excision repair gene XRCC1 and the risk of squamous cell carcinoma of the head and neck. J Exp Clin Cancer Res 2009, 28, 37. 118. Suresh, K.; Chandirasekar, R.; Kumar, B. L.; Venkatesan, R.; Sasikala, K., No association between the Trp53 codon 72 polymorphism and head and neck cancer: a case-control study in a South Indian population. Asian Pac J Cancer Prev 2010, 11, (6), 1749-53. 119. Sturgis, E. M.; Castillo, E. J.; Li, L.; Zheng, R.; Eicher, S. A.; Clayman, G. L.; Strom, S. S.; Spitz, M. R.; Wei, Q., Polymorphisms of DNA repair gene XRCC1 in squamous cell carcinoma of the head and neck. Carcinogenesis 1999, 20, (11), 2125-9. 120. Hung, R. J.; Brennan, P.; Canzian, F.; Szeszenia-Dabrowska, N.; Zaridze, D.; Lissowska, J.; Rudnai, P.; Fabianova, E.; Mates, D.; Foretova, L.; Janout, V.; Bencko, V.; Chabrier, A.; Borel, S.; Hall, J.; Boffetta, P., Large-scale investigation of base excision repair genetic polymorphisms and lung cancer risk in a multicenter study. J Natl Cancer Inst 2005, 97, (8), 567-76. 121. K. Sabitha, M. V. R. a. K. J., Mutations in XRCC1 Gene Alters the Genetic Risks of Head and Neck Cancer Patients. . International Journal of Cancer Research 2009, 5, 58-68. 122. Hung, R. J.; Hall, J.; Brennan, P.; Boffetta, P., Genetic polymorphisms in the base excision repair pathway and cancer risk: a HuGE review. Am J Epidemiol 2005, 162, (10), 925-42. 123. Shen, H.; Sturgis, E. M.; Dahlstrom, K. R.; Zheng, Y.; Spitz, M. R.; Wei, Q., A variant of the DNA repair gene XRCC3 and risk of squamous cell carcinoma of the head and neck: a casecontrol analysis. Int J Cancer 2002, 99, (6), 869-72. 124. Wen, S. X.; Zhang, X. M.; Tang, P. Z.; Zhao, D.; Guo, Y. L.; Tan, W.; Lin, D. X., [Association between genetic polymorphism in DNA repair genes XRCC3 and risks of laryngeal and hypopharyngeal carcinomas]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2007, 42, (11), 856-9. 125. Werbrouck, J.; De Ruyck, K.; Duprez, F.; Van Eijkeren, M.; Rietzschel, E.; Bekaert, S.; Vral, A.; De Neve, W.; Thierens, H., Single-nucleotide polymorphisms in DNA double-strand break repair genes: association with head and neck cancer and interaction with tobacco use and alcohol consumption. Mutat Res 2008, 656, (1-2), 74-81. 126. Gal, T. J.; Huang, W. Y.; Chen, C.; Hayes, R. B.; Schwartz, S. M., DNA repair gene polymorphisms and risk of second primary neoplasms and mortality in oral cancer patients. Laryngoscope 2005, 115, (12), 2221-31. 127. Han, S.; Zhang, H. T.; Wang, Z.; Xie, Y.; Tang, R.; Mao, Y.; Li, Y., DNA repair gene XRCC3 polymorphisms and cancer risk: a meta-analysis of 48 case-control studies. Eur J Hum Genet 2006, 14, (10), 1136-44. 128. Taylor, E. M.; Broughton, B. C.; Botta, E.; Stefanini, M.; Sarasin, A.; Jaspers, N. G.; Fawcett, H.; Harcourt, S. A.; Arlett, C. F.; Lehmann, A. R., Xeroderma pigmentosum and trichothiodystrophy are associated with different mutations in the XPD (ERCC2) repair/transcription gene. Proc Natl Acad Sci U S A 1997, 94, (16), 8658-63. 129. Shen, M. R.; Jones, I. M.; Mohrenweiser, H., Nonconservative amino acid substitution variants exist at polymorphic frequency in DNA repair genes in healthy humans. Cancer Res 1998, 58, (4), 604-8. 130. Spitz, M. R.; Wu, X.; Wang, Y.; Wang, L. E.; Shete, S.; Amos, C. I.; Guo, Z.; Lei, L.; Mohrenweiser, H.; Wei, Q., Modulation of nucleotide excision repair capacity by XPD polymorphisms in lung cancer patients. Cancer Res 2001, 61, (4), 1354-7. 155
Bibliography 131. Buch, S.; Zhu, B.; Davis, A. G.; Odom, D.; Siegfried, J. M.; Grandis, J. R.; Romkes, M., Association of polymorphisms in the cyclin D1 and XPD genes and susceptibility to cancers of the upper aero-digestive tract. Mol Carcinog 2005, 42, (4), 222-8. 132. Ramachandran, S.; Ramadas, K.; Hariharan, R.; Rejnish Kumar, R.; Radhakrishna Pillai, M., Single nucleotide polymorphisms of DNA repair genes XRCC1 and XPD and its molecular mapping in Indian oral cancer. Oral Oncol 2006, 42, (4), 350-62. 133. Yuan, H.; Niu, Y. M.; Wang, R. X.; Li, H. Z.; Chen, N., Association between XPD Lys751Gln polymorphism and risk of head and neck cancer: a meta-analysis. Genet Mol Res 2011, 10, (4). 134. Hashimoto, T.; Uchida, K.; Okayama, N.; Imate, Y.; Suehiro, Y.; Hamanaka, Y.; Ueyama, Y.; Yamashita, H.; Hinoda, Y., Interaction of OGG1 Ser326Cys polymorphism with cigarette smoking in head and neck squamous cell carcinoma. Mol Carcinog 2006, 45, (5), 344-8. 135. Kumar, A.; Pant, M. C.; Singh, H. S.; Khandelwal, S., Role of OGG1 Ser326Cys polymorphism and 8-oxoguanine DNA damage in risk assessment of squamous cell carcinoma of head and neck in North Indian population. Mutat Res 2011, 726, (2), 227-33. 136. Sliwinski, T.; Przybylowska, K.; Markiewicz, L.; Rusin, P.; Pietruszewska, W.; Zelinska- Blizniewska, H.; Olszewski, J.; Morawiec-Sztandera, A.; Mlynarski, W.; Majsterek, I., MUTYH Tyr165Cys, OGG1 Ser326Cys and XPD Lys751Gln polymorphisms and head neck cancer susceptibility: a case control study. Mol Biol Rep 2011, 38, (2), 1251-61. 137. Lee, N. K.; Ye, Y. W.; Chen, J.; Li, X.; Waber, P. G.; Nisen, P. D., p53, retinoblastoma, and human papillomavirus in squamous cell carcinoma and adjacent normal mucosa of the upper aerodigestive tract. Arch Otolaryngol Head Neck Surg 1993, 119, (10), 1125-31. 138. Wooster, R.; Bignell, G.; Lancaster, J.; Swift, S.; Seal, S.; Mangion, J.; Collins, N.; Gregory, S.;Gumbs, C.; Micklem, G., Identification of the breast cancer susceptibility gene BRCA2. Nature 1995, 378, (6559), 789-92. 139. Hirvonen, A.; Husgafvel-Pursiainen, K.; Anttila, S.; Vainio, H., The GSTM1 null genotype as a potential risk modifier for squamous cell carcinoma of the lung. Carcinogenesis 1993, 14, (7), 1479-81. 140. Smith, G.; Stanley, L. A.; Sim, E.; Strange, R. C.; Wolf, C. R., Metabolic polymorphisms and cancer susceptibility. Cancer Surv 1995, 25, 27-65. 141. Lacko, M.; Oude Ophuis, M. B.; Peters, W. H.; Manni, J. J., Genetic polymorphisms of smokingrelated carcinogen detoxifying enzymes and head and neck cancer susceptibility. Anticancer Res 2009, 29, (2), 753-61. 142. Lourenco, G. J.; Silva, E. F.; Rinck-Junior, J. A.; Chone, C. T.; Lima, C. S., CYP1A1, GSTM1 and GSTT1 polymorphisms, tobacco and alcohol status and risk of head and neck squamous cell carcinoma. Tumour Biol 2011, 32, (6), 1209-15. 143. Sam, S. S.; Thomas, V.; Reddy, K. S.; Surianarayanan, G.; Chandrasekaran, A., Geneenvironment interactions associated with CYP1A1 MspI and GST polymorphisms and the risk of upper aerodigestive tract cancers in an Indian population. J Cancer Res Clin Oncol 2010, 136, (6), 945-51. 144. Tripathy, C. B.; Roy, N., Meta-analysis of glutathione S-transferase M1 genotype and risk toward head and neck cancer. Head Neck 2006, 28, (3), 217-24. 145. Suzen, H. S.; Guvenc, G.; Turanli, M.; Comert, E.; Duydu, Y.; Elhan, A., The role of GSTM1 and GSTT1 polymorphisms in head and neck cancer risk. Oncol Res 2007, 16, (9), 423-9. 146. Evans, A. J.; Henner, W. D.; Eilers, K. M.; Montalto, M. A.; Wersinger, E. M.; Andersen, P. E.; Cohen, J. I.; Everts, E. C.; McWilliams, J. E.; Beer, T. M., Polymorphisms of GSTT1 and related genes in head and neck cancer risk. Head Neck 2004, 26, (1), 63-70. 147. Cheng, L.; Sturgis, E. M.; Eicher, S. A.; Char, D.; Spitz, M. R.; Wei, Q., Glutathione-Stransferase polymorphisms and risk of squamous-cell carcinoma of the head and neck. Int J Cancer 1999, 84, (3), 220-4. 148. Ruwali, M.; Singh, M.; Pant, M. C.; Parmar, D., Polymorphism in glutathione S-transferases: susceptibility and treatment outcome for head and neck cancer. Xenobiotica 2011, 41, (12), 1122- 30. 156
Page 1:
Genotype-Molecular Phenotype Correl
Page 7 and 8:
CONTENTS Page No Synopsis 1 List of
Page 9 and 10:
Synopsis
Page 11 and 12:
Synopsis Genotype-Molecular Phenoty
Page 13 and 14:
Synopsis Materials and Methods: 1.
Page 15 and 16:
Synopsis loading control. PCR produ
Page 17 and 18:
Synopsis 7.1 Percent cell death aft
Page 19 and 20:
Synopsis slides were then dried and
Page 21 and 22:
Synopsis range 41.77% - 90.95% at 5
Page 23 and 24:
Synopsis 5. Genotyping of genes: Ge
Page 25 and 26:
Synopsis G2/M arrest and delaying e
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Synopsis may have an important bear
Page 29 and 30:
Synopsis References: 1. Bobba, R.;
Page 31 and 32:
Abbreviations MOPS : 3-(N-morpholin
Page 33 and 34:
List of Figures Fig. 28: Percent re
Page 35 and 36:
Chapter 1 Introduction
Page 37 and 38:
Introduction In previous studies fr
Page 39 and 40:
Introduction important carcinogenes
Page 41 and 42:
Review of Literature The yet undeci
Page 43 and 44:
Review of Literature Fig. 2: Incide
Page 45 and 46:
Review of Literature sustained angi
Page 47 and 48:
Review of Literature 2.6 Genotype p
Page 49 and 50:
Review of Literature 2.7.2 Biologic
Page 51 and 52:
Review of Literature Table 1: Steps
Page 53 and 54:
Review of Literature future analysi
Page 55 and 56:
Review of Literature Inefficient ce
Page 57 and 58:
Review of Literature XRCC1 (X-ray r
Page 59 and 60:
Review of Literature 2.9.2 Gene inv
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Review of Literature MPO gene polym
Page 63 and 64:
Review of Literature important role
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Materials and Methods Source of che
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Materials and Methods Method: EBV-t
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Materials and Methods Immunophenoty
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Materials and Methods with 500 μl
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Materials and Methods cDNA it can b
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Materials and Methods Cobalt-60 iso
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Materials and Methods specific bind
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Materials and Methods h half of the
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Materials and Methods phenol and th
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Materials and Methods 7. Filter ste
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Materials and Methods viz. EXO-SAP
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Materials and Methods Table 2: List
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Materials and Methods SMAD6 AREG HM
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Materials and Methods 3.9 Effect of
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Materials and Methods fresh eppendo
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Materials and Methods Power SYBR Gr
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Results Objective 1 To generate EBV
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Results Fig. 8: Frequency of second
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Results Table 6: Demographics of he
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Results Fig. 10: Cell population do
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Results Fig. 12: Cell surface marke
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Results 4.1.5 Expression of ATM gen
Page 110 and 111:
Results Objective 2 To compare the
Page 112 and 113:
Results Fig. 17: Standardization of
Page 114 and 115:
Results Fig. 19: Standardisation of
Page 116 and 117:
Results The cells were incubated fu
Page 118 and 119: Results 4.2.3.1 Percent G2 delay af
Page 120 and 121: Results 4.2.3.2 Effect of BPDE expo
Page 122 and 123: Results Fig. 29: Comparison of perc
Page 124 and 125: Results Fig. 31: Percent cell death
Page 126 and 127: Results normalization with baseline
Page 128 and 129: Results 4.2.5.2 Real time PCR valid
Page 130 and 131: Results homozygous wild-type, heter
Page 132 and 133: Results Fig. 36b: Electrophoresis o
Page 134 and 135: Results Fig. 37a: Representative el
Page 136 and 137: Results Table 13: Consolidated geno
Page 138 and 139: Results LCL Genes NAT2(1) NAT2(2) N
Page 140 and 141: Results Fig. 38: Distribution of Ge
Page 142 and 143: Results Fig. 39: Various combinatio
Page 144 and 145: Results Table 14: Genotype-phenotyp
Page 146 and 147: Discussion Introduction Squamous ce
Page 148 and 149: Discussion stimulated lymphocytes w
Page 150 and 151: Discussion H2AX is currently the mo
Page 152 and 153: Discussion or G2/M phase arrest. As
Page 154 and 155: Discussion differential expression
Page 156 and 157: Discussion DNA repair genes and MPN
Page 158 and 159: Chapter 6 Summary and Conclusions
Page 160 and 161: Summary and Conclusions Statistical
Page 162 and 163: Bibliography 1. Dikshit, R.; Gupta,
Page 164 and 165: Bibliography 43. Lei, D.; Sturgis,
Page 166 and 167: Bibliography 76. Fry, R. C.; Svenss
Page 170 and 171: Bibliography 149. Hashibe, M.; Bren
Page 172 and 173: Bibliography 183. Bergamaschi, D.;
Page 174 and 175: Bibliography 216. Bondy, M. L.; Wan
Page 176 and 177: Indian J Med Res 135, June 2012, pp
Page 178 and 179: 822 INDIAN J MED RES, JUNE 2012 mar
Page 180 and 181: 824 INDIAN J MED RES, JUNE 2012 (h)
Page 182 and 183: 826 INDIAN J MED RES, JUNE 2012 Tab
Page 184 and 185: 828 INDIAN J MED RES, JUNE 2012 lik
Page 186 and 187: CASE REPORT Eben L. Rosenthal, MD,
Page 188 and 189: FIGURE 2. Chromosomal breakage anal
Page 190 and 191: FA along with ataxia telangiectasia
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