LIFE09200604007 Tabish - Homi Bhabha National Institute

Results
4.2.3.2 Effect of BPDE exposure on Cell cycle profile
Flow cytometry analysis of cell cycle phase distribution after BPDE exposure
revealed that there was no considerable difference in the mean percent cell arrested in S
and G2/M phase at both 6 h and 24 h time point between MPN and control cell lines.
Mean percent S phase arrest in MPN cell lines at 6 h was 18.54% at 5 µM and
19.14% at 8 µM BPDE concentrations. In control cell lines mean S phase arrest was
13.85% at 5 µM and 13.58% at 8 µM BPDE concentration. Mean percent S phase
arrest at 24 h was 19.53% at 5 µM and 20.09% at 8 µM in MPN cell lines. In control
cell lines mean S phase arrest was 17.68% at 5 µM and 16.67% at 8 µM (Fig. 27). A
comparison between the two groups in terms of difference in cells arrested in S phase
after BPDE exposure at both time points is shown in Table 11.
Mean percent G2/M arrest in MPN cell lines at 6 h was 4.86% at 5 µM and
4.87% at 8 µM BPDE concentration. In control cell lines G2/M arrest was 9.57% at 5
µM and 9.55% at 8 µM. Mean percent G2/M arrest in MPN cell lines at 24 h was
3.65% at 5 µM and 3.39% at 8 µM and control cell lines it was 5.19% at 5 µM and
4.83% at 8 µM (Fig. 28). A comparison between the two groups in terms of difference
in cells arrested in S phase after BPDE exposure at both time points is shown in Table
11 (Fig. 29).
Fig. 27: Relative S phase arrest after BPDE exposure in MPN (n=20) and control cell lines (n=10).
Percent relative S phase arrest at 6 h time point (a) percent relative S phase arrest at 24 h time point (b).
Mean difference between percent relative S phase arrest between MPN and control cell lines was not
statistically significant.
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