LIFE09200604007 Tabish - Homi Bhabha National Institute

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Results LCL Genes NAT2(1) NAT2(2) NAT2(3) BRCA2(1) BRCA2(2) GPX mEH E242 Wt Hm Wt Ht Wt Hm Hm E252 Ht Wt Wt Wt Ht Wt Hm E253 Wt Ht Wt Wt Hm Hm Ht E264 Wt Wt Wt Hm Hm Ht Wt E265 Hm Wt Wt Wt Hm Ht Wt E270 Ht Ht Wt Wt Hm Ht Wt E305 Wt Wt Wt Ht Hm Hm Ht E306 Ht Wt Wt Hm Hm Ht Ht E307 Wt Wt Ht Ht Wt Wt Wt E308 Wt Wt Wt Ht Ht Ht Ht E311 Wt Wt Wt Wt Hm Hm Wt E319 Wt Hm Ht Wt Wt Ht Wt E320 Ht Ht Hm Hm Hm Hm Ht E321 Wt Ht Ht Wt Wt Wt Wt E322 Wt Ht Wt Wt Wt Wt Ht E325 Wt Ht Ht Ht Ht Ht Ht E326 Wt Hm Wt Ht Wt Ht Wt E329 Wt Wt Wt Ht Ht Wt Wt E330 Ht Ht Ht Ht Hm Ht Ht E331 Hm Wt Wt Wt Hm Wt Wt E245 Ht Ht Wt Hm Wt Wt Ht E246 Wt Hm Wt Wt Ht Ht Ht E247 Wt Ht Wt Wt Ht Ht Ht E248 Wt Ht Wt Ht Wt Ht Ht E249 Ht Ht Wt Wt Ht Wt Hm E250 Wt Hm Wt Wt Ht Wt Hm E313 Ht Ht Wt Ht Wt Ht Ht E314 Ht Ht Ht Ht Wt Ht Wt E334 Wt Ht Wt Ht Ht Ht Ht E335 Wt Ht Wt Hm Wt Wt Wt MPN Control 128
Results 4.3.4 Genotype Score (G Score) For quantitatively analysing the genotype, a value of 2, 1 and 0 was allotted to variant, heterozygous and wildtype forms of each gene polymorphism respectively and a consolidated Genotype Score or G Score was calculated. For all samples (n=30) value of total G Score ranged from 10 - 22. A high G Score designated more number of risk alleles in an individual. Our assumption was that the individuals with a high G Score might have a higher probability of developing MPN as compared to those with the low G Score. It is evident from the genotyping data that most of the MPN patients displayed a high G Score value (≥15) while controls showed a low G Score. Of the total samples, 13/20 MPN patients had a G Score ≥15 (median total G Score) while 7/10 controls had a G Score A (5‟UTR), BRCA2-Asn 372 His); MPN risk association signature (SULT1A1-Arg 213 His, hOGG1- Ser 326 cys, BRCA2-Asn 372 His, mEH-Try 113 His, XRCC1-Arg 280 His); carcinogen metabolising enzymes genes (GSTT1-Null, GSTM1-Null, GSTP1-Ile 105 Val, SULT1A1-Arg 213 His, MPO--463G>A (promoter region), NAT2-Ile 114 Thr, NAT2- Arg 197 Gln, NAT2-Gly 286 Glu, mEH-Tyr 113 His, GPX-Pro 198 Leu); and cell cycle regulation and apoptosis genes (Fas- -670A>G (enhancer region), p53-Pro 72 Arg, p21- Ser 31 Arg, CCND1-870A>G). There was no significant difference between the G Scores of MPN and controls for carcinogen metabolising enzymes genes and cell cycle regulation and apoptosis genes subgroups (Fig. 38). However, G Score of DNA repair genes ranged from 3 - 9 and MPN risk association signature ranged from 0 - 6 and was found to be significantly higher in MPN group as compared to control group. In case of DNA repair genes 14/20 MPN patients has a G Score ≥6 (median G Score DNA repair genes) while 8/10 controls had a G Score
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Genotype-Molecular Phenotype Correl
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CONTENTS Page No Synopsis 1 List of
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Synopsis
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Synopsis Genotype-Molecular Phenoty
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Synopsis Materials and Methods: 1.
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Synopsis loading control. PCR produ
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Synopsis 7.1 Percent cell death aft
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Synopsis slides were then dried and
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Synopsis range 41.77% - 90.95% at 5
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Synopsis 5. Genotyping of genes: Ge
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Synopsis G2/M arrest and delaying e
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Synopsis may have an important bear
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Synopsis References: 1. Bobba, R.;
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Abbreviations MOPS : 3-(N-morpholin
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List of Figures Fig. 28: Percent re
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Chapter 1 Introduction
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Introduction In previous studies fr
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Introduction important carcinogenes
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Review of Literature The yet undeci
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Review of Literature Fig. 2: Incide
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Review of Literature sustained angi
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Review of Literature 2.6 Genotype p
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Review of Literature 2.7.2 Biologic
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Review of Literature Table 1: Steps
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Review of Literature future analysi
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Review of Literature Inefficient ce
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Review of Literature XRCC1 (X-ray r
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Review of Literature 2.9.2 Gene inv
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Review of Literature MPO gene polym
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Review of Literature important role
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Materials and Methods Source of che
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Materials and Methods Method: EBV-t
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Materials and Methods Immunophenoty
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Materials and Methods with 500 μl
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Materials and Methods cDNA it can b
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Materials and Methods Cobalt-60 iso
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Materials and Methods specific bind
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Materials and Methods h half of the
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Materials and Methods phenol and th
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Materials and Methods 7. Filter ste
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Materials and Methods viz. EXO-SAP
Page 88 and 89: Materials and Methods Table 2: List
Page 90 and 91: Materials and Methods SMAD6 AREG HM
Page 92 and 93: Materials and Methods 3.9 Effect of
Page 94 and 95: Materials and Methods fresh eppendo
Page 96 and 97: Materials and Methods Power SYBR Gr
Page 98 and 99: Results Objective 1 To generate EBV
Page 100 and 101: Results Fig. 8: Frequency of second
Page 102 and 103: Results Table 6: Demographics of he
Page 104 and 105: Results Fig. 10: Cell population do
Page 106 and 107: Results Fig. 12: Cell surface marke
Page 108 and 109: Results 4.1.5 Expression of ATM gen
Page 110 and 111: Results Objective 2 To compare the
Page 112 and 113: Results Fig. 17: Standardization of
Page 114 and 115: Results Fig. 19: Standardisation of
Page 116 and 117: Results The cells were incubated fu
Page 118 and 119: Results 4.2.3.1 Percent G2 delay af
Page 120 and 121: Results 4.2.3.2 Effect of BPDE expo
Page 122 and 123: Results Fig. 29: Comparison of perc
Page 124 and 125: Results Fig. 31: Percent cell death
Page 126 and 127: Results normalization with baseline
Page 128 and 129: Results 4.2.5.2 Real time PCR valid
Page 130 and 131: Results homozygous wild-type, heter
Page 132 and 133: Results Fig. 36b: Electrophoresis o
Page 134 and 135: Results Fig. 37a: Representative el
Page 136 and 137: Results Table 13: Consolidated geno
Page 140 and 141: Results Fig. 38: Distribution of Ge
Page 142 and 143: Results Fig. 39: Various combinatio
Page 144 and 145: Results Table 14: Genotype-phenotyp
Page 146 and 147: Discussion Introduction Squamous ce
Page 148 and 149: Discussion stimulated lymphocytes w
Page 150 and 151: Discussion H2AX is currently the mo
Page 152 and 153: Discussion or G2/M phase arrest. As
Page 154 and 155: Discussion differential expression
Page 156 and 157: Discussion DNA repair genes and MPN
Page 158 and 159: Chapter 6 Summary and Conclusions
Page 160 and 161: Summary and Conclusions Statistical
Page 162 and 163: Bibliography 1. Dikshit, R.; Gupta,
Page 164 and 165: Bibliography 43. Lei, D.; Sturgis,
Page 166 and 167: Bibliography 76. Fry, R. C.; Svenss
Page 168 and 169: Bibliography 115. Kote-Jarai, Z.; M
Page 170 and 171: Bibliography 149. Hashibe, M.; Bren
Page 172 and 173: Bibliography 183. Bergamaschi, D.;
Page 174 and 175: Bibliography 216. Bondy, M. L.; Wan
Page 176 and 177: Indian J Med Res 135, June 2012, pp
Page 178 and 179: 822 INDIAN J MED RES, JUNE 2012 mar
Page 180 and 181: 824 INDIAN J MED RES, JUNE 2012 (h)
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Page 186 and 187: CASE REPORT Eben L. Rosenthal, MD,
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FIGURE 2. Chromosomal breakage anal
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FA along with ataxia telangiectasia
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LIFE09200604007 Tabish - Homi Bhabha National Institute

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