LIFE09200604007 Tabish - Homi Bhabha National Institute

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Bibliography 216. Bondy, M. L.; Wang, L. E.; El-Zein, R.; de Andrade, M.; Selvan, M. S.; Bruner, J. M.; Levin, V. A.; Alfred Yung, W. K.; Adatto, P.; Wei, Q., Gamma-radiation sensitivity and risk of glioma. J Natl Cancer Inst 2001, 93, (20), 1553-7. 217. Mahabir, S.; Wei, Q.; Barrera, S. L.; Dong, Y. Q.; Etzel, C. J.; Spitz, M. R.; Forman, M. R., Dietary magnesium and DNA repair capacity as risk factors for lung cancer. Carcinogenesis 2008, 29, (5), 949- 56. 218. Li, D.; Firozi, P. F.; Wang, L. E.; Bosken, C. H.; Spitz, M. R.; Hong, W. K.; Wei, Q., Sensitivity to DNA damage induced by benzo(a)pyrene diol epoxide and risk of lung cancer: a case-control analysis. Cancer Res 2001, 61, (4), 1445-50. 219. Kuo, L. J.; Yang, L. X., Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In Vivo 2008, 22, (3), 305-9. 220. Huang, X.; Darzynkiewicz, Z., Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage. Methods Mol Biol 2006, 314, 73-80. 221. Muslimovic, A.; Ismail, I. H.; Gao, Y.; Hammarsten, O., An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells. Nat Protoc 2008, 3, (7), 1187-93. 222. Kotsopoulos, J.; Chen, Z.; Vallis, K. A.; Poll, A.; Ainsworth, P.; Narod, S. A., DNA repair capacity as a possible biomarker of breast cancer risk in female BRCA1 mutation carriers. Br J Cancer 2007, 96, (1), 118-25. 223. Spitz, M. R.; Lippman, S. M.; Jiang, H.; Lee, J. J.; Khuri, F.; Hsu, T. C.; Trizna, Z.; Schantz, S. P.; Benner, S.; Hong, W. K., Mutagen sensitivity as a predictor of tumor recurrence in patients with cancer of the upper aerodigestive tract. J Natl Cancer Inst 1998, 90, (3), 243-5. 224. Spitz, M. R.; McPherson, R. S.; Jiang, H.; Hsu, T. C.; Trizna, Z.; Lee, J. J.; Lippman, S. M.; Khuri, F. R.; Steffen-Batey, L.; Chamberlain, R. M.; Schantz, S. P.; Hong, W. K., Correlates of mutagen sensitivity in patients with upper aerodigestive tract cancer. Cancer Epidemiol Biomarkers Prev 1997, 6, (9), 687-92. 225. Weinert, T.; Lydall, D., Cell cycle checkpoints, genetic instability and cancer. Semin Cancer Biol 1993, 4, (2), 129-40. 226. Kaufmann, W. K.; Paules, R. S., DNA damage and cell cycle checkpoints. FASEB J 1996, 10, (2), 238-47. 227. Xiao, H.; Singh, S. V., p53 regulates cellular responses to environmental carcinogen benzo[a]pyrene-7,8-diol-9,10-epoxide in human lung cancer cells. Cell Cycle 2007, 6, (14), 1753- 61. 228. Denissenko, M. F.; Pao, A.; Tang, M.; Pfeifer, G. P., Preferential formation of benzo[a]pyrene adducts at lung cancer mutational hotspots in P53. Science 1996, 274, (5286), 430-2. 229. Meigs, J. B.; Shrader, P.; Sullivan, L. M.; McAteer, J. B.; Fox, C. S.; Dupuis, J.; Manning, A. K.; Florez, J. C.; Wilson, P. W.; D'Agostino, R. B., Sr.; Cupples, L. A., Genotype score in addition to common risk factors for prediction of type 2 diabetes. N Engl J Med 2008, 359, (21), 2208-19. 230. Kathiresan, S.; Melander, O.; Anevski, D.; Guiducci, C.; Burtt, N. P.; Roos, C.; Hirschhorn, J. N.; Berglund, G.; Hedblad, B.; Groop, L.; Altshuler, D. M.; Newton-Cheh, C.; Orho-Melander, M., Polymorphisms associated with cholesterol and risk of cardiovascular events. N Engl J Med 2008, 358, (12), 1240-9. 231. Oguri, T.; Singh, S. V.; Nemoto, K.; Lazo, J. S.; The carcinogen (7R,8S)-dihydroxy-(9S,10R)- epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene induces Cdc25B expression in human bronchial and lung cancer cells. Cancer Res 2003;63, (4) 771-5. 161
Publications Publications 1. Hussain T, Kotnis A, Sarin R and Mulherkar R. Establishment and characterization of lymphoblastoid cell lines from patients with Multiple Primary Neoplasms in the upper aero-digestive tract and healthy individuals. Indian J Med Res. 2012 Jun; 135(6):820-9. 2. Budrukkar A, Shahid T, Murthy V, Hussain T, Mulherkar R, Vundinti BR, Deshpande M, Sengar M, Laskar SG and Agarwal JP. Squamous cell carcinoma of base of tongue in a patient with Fanconi's anemia treated with radiation therapy: case report and review of literature. Head & Neck, 2010 Oct; 32(10):1422-7. 3. Hussain T and Mulherkar R. Lymphoblastoid cell lines: a continuous in-vitro source to study carcinogen sensitivity and DNA repair. (Review article; International Journal of Cellular and Molecular Medicine, in press). 4. Hussain T, Kotnis A, Sarin R and Mulherkar R. Genetic Susceptibility to Multiple Primary Neoplasms in the Upper Aero-Digestive Tract: Genotype Score and Phenotype Correlation. (communicated to Cancer letters) Poster/oral presentations 1. International Society for Cell & Gene Therapy of Cancer (ISCGT) conference, India, 2007. (Participation) 2. 13 th Human Genome Meeting “Genomics and the future of medicine”, India, 2008. (Poster presentation) 3. 29 th Annual Convention of Indian Association for Cancer Research, India, 2010. (Oral presentation) 4. 6 th Graduate Students Meet, ACTREC, Navi Mumbai, 2010. (Poster award) 5. 30 th Annual Convention of Indian Association for Cancer Research and International Symposium on “Signalling Network and Cancer”, CSIR-IICB, Kolkata, 2011. („Rajnikant Baxi‟ poster award) 6. 1 st Global Cancer Genomics Consortium-Tata Memorial Centre, 2011. (Poster award). 7. American Association of Cancer Research (AACR) international conference on “New Horizons in Cancer Research: Biology to prevention to Therapy”, 2011. (Poster presentation) 162
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Genotype-Molecular Phenotype Correl
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CONTENTS Page No Synopsis 1 List of
Page 9 and 10:
Synopsis
Page 11 and 12:
Synopsis Genotype-Molecular Phenoty
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Synopsis Materials and Methods: 1.
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Synopsis loading control. PCR produ
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Synopsis 7.1 Percent cell death aft
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Synopsis slides were then dried and
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Synopsis range 41.77% - 90.95% at 5
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Synopsis 5. Genotyping of genes: Ge
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Synopsis G2/M arrest and delaying e
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Synopsis may have an important bear
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Synopsis References: 1. Bobba, R.;
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Abbreviations MOPS : 3-(N-morpholin
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List of Figures Fig. 28: Percent re
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Chapter 1 Introduction
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Introduction In previous studies fr
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Introduction important carcinogenes
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Review of Literature The yet undeci
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Review of Literature Fig. 2: Incide
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Review of Literature sustained angi
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Review of Literature 2.6 Genotype p
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Review of Literature 2.7.2 Biologic
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Review of Literature Table 1: Steps
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Review of Literature future analysi
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Review of Literature Inefficient ce
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Review of Literature XRCC1 (X-ray r
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Review of Literature 2.9.2 Gene inv
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Review of Literature MPO gene polym
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Review of Literature important role
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Materials and Methods Source of che
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Materials and Methods Method: EBV-t
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Materials and Methods Immunophenoty
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Materials and Methods with 500 μl
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Materials and Methods cDNA it can b
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Materials and Methods Cobalt-60 iso
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Materials and Methods specific bind
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Materials and Methods h half of the
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Materials and Methods phenol and th
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Materials and Methods 7. Filter ste
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Materials and Methods viz. EXO-SAP
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Materials and Methods Table 2: List
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Materials and Methods SMAD6 AREG HM
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Materials and Methods 3.9 Effect of
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Materials and Methods fresh eppendo
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Materials and Methods Power SYBR Gr
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Results Objective 1 To generate EBV
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Results Fig. 8: Frequency of second
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Results Table 6: Demographics of he
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Results Fig. 10: Cell population do
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Results Fig. 12: Cell surface marke
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Results 4.1.5 Expression of ATM gen
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Results Objective 2 To compare the
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Results Fig. 17: Standardization of
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Results Fig. 19: Standardisation of
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Results The cells were incubated fu
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Results 4.2.3.1 Percent G2 delay af
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Results 4.2.3.2 Effect of BPDE expo
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Results Fig. 29: Comparison of perc
Page 124 and 125: Results Fig. 31: Percent cell death
Page 126 and 127: Results normalization with baseline
Page 128 and 129: Results 4.2.5.2 Real time PCR valid
Page 130 and 131: Results homozygous wild-type, heter
Page 132 and 133: Results Fig. 36b: Electrophoresis o
Page 134 and 135: Results Fig. 37a: Representative el
Page 136 and 137: Results Table 13: Consolidated geno
Page 138 and 139: Results LCL Genes NAT2(1) NAT2(2) N
Page 140 and 141: Results Fig. 38: Distribution of Ge
Page 142 and 143: Results Fig. 39: Various combinatio
Page 144 and 145: Results Table 14: Genotype-phenotyp
Page 146 and 147: Discussion Introduction Squamous ce
Page 148 and 149: Discussion stimulated lymphocytes w
Page 150 and 151: Discussion H2AX is currently the mo
Page 152 and 153: Discussion or G2/M phase arrest. As
Page 154 and 155: Discussion differential expression
Page 156 and 157: Discussion DNA repair genes and MPN
Page 158 and 159: Chapter 6 Summary and Conclusions
Page 160 and 161: Summary and Conclusions Statistical
Page 162 and 163: Bibliography 1. Dikshit, R.; Gupta,
Page 164 and 165: Bibliography 43. Lei, D.; Sturgis,
Page 166 and 167: Bibliography 76. Fry, R. C.; Svenss
Page 168 and 169: Bibliography 115. Kote-Jarai, Z.; M
Page 170 and 171: Bibliography 149. Hashibe, M.; Bren
Page 172 and 173: Bibliography 183. Bergamaschi, D.;
Page 176 and 177: Indian J Med Res 135, June 2012, pp
Page 178 and 179: 822 INDIAN J MED RES, JUNE 2012 mar
Page 180 and 181: 824 INDIAN J MED RES, JUNE 2012 (h)
Page 182 and 183: 826 INDIAN J MED RES, JUNE 2012 Tab
Page 184 and 185: 828 INDIAN J MED RES, JUNE 2012 lik
Page 186 and 187: CASE REPORT Eben L. Rosenthal, MD,
Page 188 and 189: FIGURE 2. Chromosomal breakage anal
Page 190 and 191: FA along with ataxia telangiectasia
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