Bibliography 1. Dikshit, R.; Gupta, P. C.; Ramasundarahettige, C.; Gajalakshmi, V.; Aleksandrowicz, L.; Badwe, R.; Kumar, R.; Roy, S.; Suraweera, W.; Bray, F.; Mallath, M.; Singh, P. K.; Sinha, D. N.; Shet, A. S.; Gelband, H.; Jha, P., Cancer mortality in India: a nationally representative survey. Lancet 2012. [Epub ahead of print] 2. Cancer Incidence, Mortality and Prevalence Worldwide in 2008. GLOBOCAN 2008. 3. Bobba, R.; Khan, Y., Cancer in India – An Overview. GOR 2003, 5, (4), 93-96. 4. Priante, A. V.; Castilho, E. C.; Kowalski, L. P., Second primary tumors in patients with head and neck cancer. Curr Oncol Rep 2011, 13, (2), 132-7. 5. Ogden, G. R., Second malignant tumours in head and neck cancer. BMJ 1991, 302, (6770), 193-4. 6. Boysen, M.; Loven, J. O., Second malignant neoplasms in patients with head and neck squamous cell carcinomas. Acta Oncol 1993, 32, (3), 283-8. 7. Jhavar, S.; Sarin, R.; Mulherkar, R.; Benner, A.; Agarwal, J. P.; Dinshaw, K., Glutathione S- transferase M1 or T1 null genotype as a risk factor for developing multiple primary neoplasms in the upper aero-digestive tract, in Indian males using tobacco. Oral Oncol 2004, 40, (1), 84-91. 8. Khuri, F. R.; Kim, E. S.; Lee, J. J.; Winn, R. J.; Benner, S. E.; Lippman, S. M.; Fu, K. K.; Cooper, J. S.; Vokes, E. E.; Chamberlain, R. M.; Williams, B.; Pajak, T. F.; Goepfert, H.; Hong, W. K., The impact of smoking status, disease stage, and index tumor site on second primary tumor incidence and tumor recurrence in the head and neck retinoid chemoprevention trial. Cancer Epidemiol Biomarkers Prev 2001, 10, (8), 823-9. 9. Kotnis, A.; Sarin, R.; Mulherkar, R., Genotype, phenotype and cancer: role of low penetrance genes and environment in tumour susceptibility. J Biosci 2005, 30, (1), 93-102. 10. Rydzanicz, M.; Wierzbicka, M.; Gajecka, M.; Szyfter, W.; Szyfter, K., The impact of genetic factors on the incidence of multiple primary tumors (MPT) of the head and neck. Cancer Lett 2005, 224, (2), 263-78. 11. Kotnis, A.; Namkung, J.; Kannan, S.; Jayakrupakar, N.; Park, T.; Sarin, R.; Mulherkar, R., Multiple pathway-based genetic variations associated with tobacco related multiple primary neoplasms. PLoS One 2012, 7, (1), e30013. 12. Kotnis, A.; Kannan, S.; Sarin, R.; Mulherkar, R., Case-control study and meta-analysis of SULT1A1 Arg213His polymorphism for gene, ethnicity and environment interaction for cancer risk. Br J Cancer 2008, 99, (8), 1340-7. 13. Muir, C.; Weiland, L., Upper aerodigestive tract cancers. Cancer 1995, 75, (1 Suppl), 147-53. 14. Tobias, J. S., Cancer of the head and neck. BMJ 1994, 308, (6934), 961-6. 15. Pelucchi, C.; Gallus, S.; Garavello, W.; Bosetti, C.; La Vecchia, C., Cancer risk associated with alcohol and tobacco use: focus on upper aero-digestive tract and liver. Alcohol Res Health 2006, 29, (3), 193-8. 16. World Cancer Report 2008. World Health Organization 2008. 17. Kotwall, C.; Razack, M. S.; Sako, K.; Rao, U., Multiple primary cancers in squamous cell cancer of the head and neck. J Surg Oncol 1989, 40, (2), 97-9. 18. Wu, X.; Gu, J.; Dong, Q.; Huang, M.; Do, K. A.; Hong, W. K.; Spitz, M. R., Joint effect of mutagen sensitivity and insulin-like growth factors in predicting the risk of developing secondary primary tumors and tumor recurrence in patients with head and neck cancer. Clin Cancer Res 2006, 12, (23), 7194-201. 19. Zafereo, M. E.; Sturgis, E. M.; Liu, Z.; Wang, L. E.; Wei, Q.; Li, G., Nucleotide excision repair core gene polymorphisms and risk of second primary malignancy in patients with index squamous cell carcinoma of the head and neck. Carcinogenesis 2009, 30, (6), 997-1002. 20. Lefebvre, J. L., Current clinical outcomes demand new treatment options for SCCHN. Ann Oncol 2005, 16 Suppl 6, vi7-vi12. 21. Joseph, F.; Fraumeni, Jr.; Curtis, R. E.; Edwards, B. K.; Tucker, M. A., New malignancies among cancer survivors: SEER cancer registries, 1973–2000. 149
Bibliography 22. Billroth, T., Die Allgemeine Chirurgische Pathologie und Therapie in 51 Vorlesungen. In: Handbuch fur Studierende and Artze, 14. Berlin: Auflage. 1889, 908. 23. Warren S.; Gates, O., Multiple primary malignant tumors: a survey of the literature and a statistical study. Am J Cancer 1932, 16, 1358-1414. 24. Day, G. L.; Blot, W. J., Second primary tumors in patients with oral cancer. Cancer 1992, 70, (1), 14-9. 25. Luo, G.; Santoro, I. M.; McDaniel, L. D.; Nishijima, I.; Mills, M.; Youssoufian, H.; Vogel, H.; Schultz, R. A.; Bradley, A., Cancer predisposition caused by elevated mitotic recombination in Bloom mice. Nat Genet 2000, 26, (4), 424-9. 26. Kupfer, G. M.; Naf, D.; D'Andrea, A. D., Molecular biology of Fanconi anemia. Hematol Oncol Clin North Am 1997, 11, (6), 1045-60. 27. Tonin, P. N., Genes implicated in hereditary breast cancer syndromes. Semin Surg Oncol 2000, 18, (4), 281-6. 28. Varley, J. M., Germline TP53 mutations and Li-Fraumeni syndrome. Hum Mutat 2003, 21, (3), 313-20. 29. Slaughter, D. P.; Southwick, H. W.; Smejkal, W., Field cancerization in oral stratified squamous epithelium; clinical implications of multicentric origin. Cancer 1953, 6, (5), 963-8. 30. Braakhuis, B. J.; Tabor, M. P.; Kummer, J. A.; Leemans, C. R.; Brakenhoff, R. H., A genetic explanation of Slaughter's concept of field cancerization: evidence and clinical implications. Cancer Res 2003, 63, (8), 1727-30. 31. Hanahan, D.; Weinberg, R. A., Hallmarks of cancer: the next generation. Cell 2011, 144, (5), 646- 74. 32. Smith, E. M.; Rubenstein, L. M.; Haugen, T. H.; Pawlita, M.; Turek, L. P., Complex etiology underlies risk and survival in head and neck cancer human papillomavirus, tobacco, and alcohol: a case for multifactor disease. J Oncol 2012, 2012, 571862. 33. Fan, C. Y., Genetic alterations in head and neck cancer: interactions among environmental carcinogens, cell cycle control, and host DNA repair. Curr Oncol Rep 2001, 3, (1), 66-71. 34. Yamasaki, H.; Mironov, N., Genomic instability in multistage carcinogenesis. Toxicol Lett 2000, 112-113, 251-6. 35. Baez, A., Genetic and environmental factors in head and neck cancer genesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2008, 26, (2), 174-200. 36. Doll, R., Pott and the path to prevention. Arch Geschwulstforsch 1975, 45, (6), 521-31. 37. Cogliano, V. J.; Baan, R.; Straif, K.; Grosse, Y.; Lauby-Secretan, B.; El Ghissassi, F.; Bouvard, V.; Benbrahim-Tallaa, L.; Guha, N.; Freeman, C.; Galichet, L.; Wild, C. P., Preventable exposures associated with human cancers. J Natl Cancer Inst 2011, 103, (24), 1827-39. 38. Olshan, A. F.; Weissler, M. C.; Watson, M. A.; Bell, D. A., GSTM1, GSTT1, GSTP1, CYP1A1, and NAT1 polymorphisms, tobacco use, and the risk of head and neck cancer. Cancer Epidemiol Biomarkers Prev 2000, 9, (2), 185-91. 39. Houlston, R. S.; Peto, J., The search for low-penetrance cancer susceptibility alleles. Oncogene 2004, 23, (38), 6471-6. 40. Kotnis, A.; Namkung, J.; Kannan, S.; Jayakrupakar, N.; Park, T.; Sarin, R.; Mulherkar, R., Multiple pathway-based genetic variations associated with tobacco related multiple primary neoplasms. PLoS One 7, (1), e30013. 41. Li, F.; Sturgis, E. M.; Chen, X.; Zafereo, M. E.; Wei, Q.; Li, G., Association of p53 codon 72 polymorphism with risk of second primary malignancy in patients with squamous cell carcinoma of the head and neck. Cancer 2010, 116, (10), 2350-9. 42. Lei, D.; Sturgis, E. M.; Wang, L. E.; Liu, Z.; Zafereo, M. E.; Wei, Q.; Li, G., FAS and FASLG genetic variants and risk for second primary malignancy in patients with squamous cell carcinoma of the head and neck. Cancer Epidemiol Biomarkers Prev 2010, 19, (6), 1484-91. 150
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Genotype-Molecular Phenotype Correl
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CONTENTS Page No Synopsis 1 List of
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Synopsis
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Synopsis Genotype-Molecular Phenoty
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Synopsis Materials and Methods: 1.
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Synopsis loading control. PCR produ
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Synopsis 7.1 Percent cell death aft
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Synopsis slides were then dried and
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Synopsis range 41.77% - 90.95% at 5
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Synopsis 5. Genotyping of genes: Ge
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Synopsis G2/M arrest and delaying e
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Synopsis may have an important bear
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Synopsis References: 1. Bobba, R.;
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Abbreviations MOPS : 3-(N-morpholin
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List of Figures Fig. 28: Percent re
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Chapter 1 Introduction
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Introduction In previous studies fr
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Introduction important carcinogenes
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Review of Literature The yet undeci
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Review of Literature Fig. 2: Incide
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Review of Literature sustained angi
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Review of Literature 2.7.2 Biologic
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Review of Literature Table 1: Steps
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Review of Literature future analysi
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Review of Literature 2.9.2 Gene inv
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Review of Literature MPO gene polym
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Review of Literature important role
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Materials and Methods Source of che
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Materials and Methods Method: EBV-t
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Materials and Methods Immunophenoty
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Materials and Methods cDNA it can b
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Results Objective 1 To generate EBV
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Results Fig. 8: Frequency of second
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Results Table 6: Demographics of he
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Results 4.1.5 Expression of ATM gen
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Results Objective 2 To compare the
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