LIFE09200604007 Tabish - Homi Bhabha National Institute
LIFE09200604007 Tabish - Homi Bhabha National Institute
LIFE09200604007 Tabish - Homi Bhabha National Institute
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Materials and Methods<br />
Source of chemicals, reagents and consumables<br />
1. Common salts, buffers, detergents, organic reagents: USB corp./ Sigma Chemical<br />
Co./ Sigma Aldrich Inc./ Invitrogen, USA; Jebsen & Jessen, GmbH & Co., Germany;<br />
BDH AnalaR, UK; Qualigens fine chemicals/ Thomas Baker Chemical Ltd./ SD Fine-<br />
Chem. Ltd./Merck India Ltd., India.<br />
2. DNA/ RNA detection: USB corp./ Sigma Chemical Co./ Sigma Aldrich Inc., USA;<br />
Genei, India.<br />
3. Antibodies: Millipore/ Cell Signalling Tech./ Molecular Probes-Invitrogen/ BD<br />
Parmingen, USA; Roche Diagnostics GmbH, Germany.<br />
4. PCR/ Quantitative Real-Time PCR/ cDNA synthesis/ Restriction and other enzymes/<br />
Snapshot reagents/ DNA free kit: MBI Fermentas Canada Inc., Canada; Applied<br />
Biosystems/ USB corp./ Axygen/ Sigma Aldrich Inc., St. Louis/ Invitrogen, USA; New<br />
England Biolabs Inc. (UK) Ltd., UK.<br />
5. Reagents for mammalian cell culture: Gibco-Invitrogen/ Sigma Aldrich Inc., USA.<br />
6. B95-8 cell line: <strong>National</strong> Centre for Cell Science, Pune, India.<br />
7. Tissue culture plastic wares, filters & centrifuge tubes: Nunc, Denmark; Corning/ BD<br />
biosciences/ Falcon/ Millipore/ Nalgene/Thermo-Fisher, USA.<br />
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