LIFE09200604007 Tabish - Homi Bhabha National Institute

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Results Fig. 29: Comparison of percent S and G2/M phase arrest between MPN and control groups after BPDE exposure. There difference between mean percent cell arrested in S (a) and G2/M phase (b) at both 6 h and 24 h time points is no statistically significant between the two groups. The difference was not statistically significant between the two groups at both BPDE concentrations and both time points. Vertical line on each bar shows standard deviation. 4.2.4 Apoptotic response in MPN and control samples in vitro A method of Annexin-V-PI double labelling was used to measure percent cell death after γ-radiation and BPDE exposure. The apoptotic response was performed on LCLs from 20 MPN and 10 controls. 4.2.4.1 Percent cell death after γ-radiation exposure Percent cell death after radiation exposure was higher in control cell lines with average percent cell death of 19.22% at 5 Gy and 22.09% at 10 Gy, as compared to MPN cell lines where the average percent cell death was 9.84% at 5 Gy and 10.84% at 10 Gy. The mean difference between the two groups was 9.38% at 5 Gy and 11.25% at 10 Gy which was statistically significant with a p value=0.0003 at 5 Gy and p=0.0002 at 10 Gy (Fig. 30). A comparison between the two groups in terms of percent cell death after γ-radiation exposure at both radiation doses is shown in Table 12 (Fig. 32). 112
Results Fig. 30: Percent cell death after radiation exposure in MPN (n=20) and control cell lines (n=10). Percent cell death after 5 Gy radiation exposure (a) percent cell death after 10 Gy radiation exposure (b). Mean difference between percent cell death between MPN and control cell lines was statistically significant (p=0.0003 at 5 Gy and p=0.0002 at 10 Gy). The values shown are the mean values from three separate experiments. 4.2.4.2 Percent cell death after BPDE exposure A statistically significant difference was observed in percent cell death between MPN and control cell lines on exposure to BPDE. Percent cell death was higher in control cell lines when compared to MPN cell lines (Fig. 31). The results demonstrate that the mean BPDE induced percent cell death was 23.25% at 5 µM and 24.98% 8 µM in control cell lines which was higher as compared to MPN cell lines where mean percent cell death was 13.57% at 5 µM and 14.02% at 8 µM BPDE concentrations. Untreated cells and cells treated with THF were taken as control. The mean difference between the two groups was 9.68% at 5 µM and 10.98% at 8 µM which was statistically significant with p value=0.0009 at 5 µM and 8 µM BPDE concentration. A comparison between the two groups in terms of percent cell death after γ-radiation exposure and after BPDE exposure is shown in Table 12 (Fig.27). 113
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Genotype-Molecular Phenotype Correl
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CONTENTS Page No Synopsis 1 List of
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Synopsis
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Synopsis Genotype-Molecular Phenoty
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Synopsis Materials and Methods: 1.
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Synopsis loading control. PCR produ
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Synopsis 7.1 Percent cell death aft
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Synopsis slides were then dried and
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Synopsis range 41.77% - 90.95% at 5
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Synopsis 5. Genotyping of genes: Ge
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Synopsis G2/M arrest and delaying e
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Synopsis may have an important bear
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Synopsis References: 1. Bobba, R.;
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Abbreviations MOPS : 3-(N-morpholin
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List of Figures Fig. 28: Percent re
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Chapter 1 Introduction
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Introduction In previous studies fr
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Introduction important carcinogenes
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Review of Literature The yet undeci
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Review of Literature Fig. 2: Incide
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Review of Literature sustained angi
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Review of Literature 2.6 Genotype p
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Review of Literature 2.7.2 Biologic
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Review of Literature Table 1: Steps
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Review of Literature future analysi
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Review of Literature Inefficient ce
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Review of Literature XRCC1 (X-ray r
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Review of Literature 2.9.2 Gene inv
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Review of Literature MPO gene polym
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Review of Literature important role
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Materials and Methods Source of che
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Materials and Methods Method: EBV-t
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Materials and Methods Immunophenoty
Page 71 and 72: Materials and Methods with 500 μl
Page 73 and 74: Materials and Methods cDNA it can b
Page 75 and 76: Materials and Methods Cobalt-60 iso
Page 77 and 78: Materials and Methods specific bind
Page 79 and 80: Materials and Methods h half of the
Page 81 and 82: Materials and Methods phenol and th
Page 83 and 84: Materials and Methods 7. Filter ste
Page 85 and 86: Materials and Methods viz. EXO-SAP
Page 88 and 89: Materials and Methods Table 2: List
Page 90 and 91: Materials and Methods SMAD6 AREG HM
Page 92 and 93: Materials and Methods 3.9 Effect of
Page 94 and 95: Materials and Methods fresh eppendo
Page 96 and 97: Materials and Methods Power SYBR Gr
Page 98 and 99: Results Objective 1 To generate EBV
Page 100 and 101: Results Fig. 8: Frequency of second
Page 102 and 103: Results Table 6: Demographics of he
Page 104 and 105: Results Fig. 10: Cell population do
Page 106 and 107: Results Fig. 12: Cell surface marke
Page 108 and 109: Results 4.1.5 Expression of ATM gen
Page 110 and 111: Results Objective 2 To compare the
Page 112 and 113: Results Fig. 17: Standardization of
Page 114 and 115: Results Fig. 19: Standardisation of
Page 116 and 117: Results The cells were incubated fu
Page 118 and 119: Results 4.2.3.1 Percent G2 delay af
Page 120 and 121: Results 4.2.3.2 Effect of BPDE expo
Page 124 and 125: Results Fig. 31: Percent cell death
Page 126 and 127: Results normalization with baseline
Page 128 and 129: Results 4.2.5.2 Real time PCR valid
Page 130 and 131: Results homozygous wild-type, heter
Page 132 and 133: Results Fig. 36b: Electrophoresis o
Page 134 and 135: Results Fig. 37a: Representative el
Page 136 and 137: Results Table 13: Consolidated geno
Page 138 and 139: Results LCL Genes NAT2(1) NAT2(2) N
Page 140 and 141: Results Fig. 38: Distribution of Ge
Page 142 and 143: Results Fig. 39: Various combinatio
Page 144 and 145: Results Table 14: Genotype-phenotyp
Page 146 and 147: Discussion Introduction Squamous ce
Page 148 and 149: Discussion stimulated lymphocytes w
Page 150 and 151: Discussion H2AX is currently the mo
Page 152 and 153: Discussion or G2/M phase arrest. As
Page 154 and 155: Discussion differential expression
Page 156 and 157: Discussion DNA repair genes and MPN
Page 158 and 159: Chapter 6 Summary and Conclusions
Page 160 and 161: Summary and Conclusions Statistical
Page 162 and 163: Bibliography 1. Dikshit, R.; Gupta,
Page 164 and 165: Bibliography 43. Lei, D.; Sturgis,
Page 166 and 167: Bibliography 76. Fry, R. C.; Svenss
Page 168 and 169: Bibliography 115. Kote-Jarai, Z.; M
Page 170 and 171: Bibliography 149. Hashibe, M.; Bren
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Bibliography 183. Bergamaschi, D.;
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Bibliography 216. Bondy, M. L.; Wan
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Indian J Med Res 135, June 2012, pp
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822 INDIAN J MED RES, JUNE 2012 mar
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824 INDIAN J MED RES, JUNE 2012 (h)
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826 INDIAN J MED RES, JUNE 2012 Tab
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828 INDIAN J MED RES, JUNE 2012 lik
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CASE REPORT Eben L. Rosenthal, MD,
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FIGURE 2. Chromosomal breakage anal
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FA along with ataxia telangiectasia
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LIFE09200604007 Tabish - Homi Bhabha National Institute

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