WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm
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DNA Fragmentati<strong>on</strong> Index (DFI)<br />
Several studies reported an associati<strong>on</strong> between <str<strong>on</strong>g>in</str<strong>on</strong>g>creased<br />
sperm aneuploidy and <str<strong>on</strong>g>in</str<strong>on</strong>g>creased DFI<br />
Muriel et al, 2007; Perr<str<strong>on</strong>g>in</str<strong>on</strong>g> et al, 2008 and 2011; Enciso et al, 2013<br />
That aneuploidy could trigger DNA fragmentati<strong>on</strong> via an<br />
apoptotic like process mediated by endogenous nucleases<br />
Enciso et al, 2013<br />
Br<strong>on</strong>net et al (2012) failed to show any correlati<strong>on</strong> between DFI<br />
and the aneuploidy rate <str<strong>on</strong>g>in</str<strong>on</strong>g> embryos produced from couples<br />
with RPL or RIF<br />
Hands-<strong>on</strong> Workshops, Thessal<strong>on</strong>iki, 30.08 – 05.09 2014<br />
33<br />
Paternal Age<br />
It is biologically plausible that chromosomal n<strong>on</strong>disjuncti<strong>on</strong><br />
errors should <str<strong>on</strong>g>in</str<strong>on</strong>g>crease with age<br />
Epidiomiological and the majority of other related studies<br />
carried out so far failed to determ<str<strong>on</strong>g>in</str<strong>on</strong>g>e the suspected<br />
negative effect of age <strong>on</strong> the frequency of disomic sperm<br />
Buwe et al, 2005; F<strong>on</strong>seka et al, 2011; Plastira et al, 2007<br />
Hands-<strong>on</strong> Workshops, Thessal<strong>on</strong>iki, 30.08 – 05.09 2014<br />
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