WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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72 General <strong>in</strong>formation Target Audience Embryolab Academy Hands-on Workshops are designed for reproductive scientists, embryologists, lab technicians, geneticists and cl<strong>in</strong>icians who desire to improve their knowledge and practical skills <strong>in</strong> specialised laboratory techniques used <strong>in</strong> a human ART sett<strong>in</strong>g. Prom<strong>in</strong>ent teachers Embryolab Academy strives to provide evidence based scientific knowledge presented by an <strong>in</strong>ternational panel of prom<strong>in</strong>ent experts. Our teachers have been carefully selected on the basis of the importance and weight of their scientific work <strong>in</strong> the specific doma<strong>in</strong>s of <strong>in</strong>terest as well as their excellent educational skills and their day to day hands-on experience <strong>in</strong> different laboratory techniques <strong>in</strong> IVF. Venue The Hands-on Workshops will be held at Embryolab Academy, 173-175 Ethnikis Antistaseos, 551 34 Kalamaria, Thessaloniki, Greece. Transport to Embryolab Academy dur<strong>in</strong>g your Workshop Transport from the City hotel to and from the Workshop Venue is <strong>in</strong>cluded <strong>in</strong> the registration fee. Embryolab Academy will confirm morn<strong>in</strong>g time and place of departure at your hotel. Thessaloniki Thessaloniki is the second-largest city <strong>in</strong> Greece and the capital of the region of Central Macedonia. Greece’s second major economic, <strong>in</strong>dustrial, commercial and political centre and a major transportation hub for the rest of southeastern Europe. The city is renowned for its festivals, events and vibrant cultural life <strong>in</strong> general, and is considered to be Greece’s cultural capital. Climate Day temperatures <strong>in</strong> September range from 15°C to 30°C, overall days are sunny and dry. Workshop language The official language of the Workshop is English. Liability The Workshop secretariat and organiser cannot assume liability for personal accidents, loss of or damage to private property of participants, either dur<strong>in</strong>g, or directly aris<strong>in</strong>g from the Workshop. Participants should make their own arrangements with respect to health and travel <strong>in</strong>surance. Embryolab Academy cannot be held accountable for cancellation of participation because of air strikes or serious adverse events. Time zone The time zone <strong>in</strong> Greece is Eastern Europe Time Zone: UTC/GMT +2 hours.
73 Certificate of attendance A Certificate of attendance will be awarded to the delegates after attend<strong>in</strong>g the full workshop programme. After complet<strong>in</strong>g the evaluation form of the workshop, your certificates will be awarded to you. The Hands-on Workshops are runn<strong>in</strong>g under the auspices, ASEBIR, the Spanish Society for the Study of Biology of Reproduction Workshop Secretariat Embryolab Academy 173-175 Ethnikis Antistaseos, 551 34 Kalamaria, Thessaloniki, Greece <strong>in</strong>fo@embryolab-academy.org www.embryolab-academy.org Workshop Venue Embryolab Academy at Embryolab – Campus, Thessaloniki, Greece 173-175 Ethnikis Antistaseos, 551 34 Kalamaria, Thessaloniki, Greece <strong>in</strong>fo@embryolab-academy.org www.embryolab-academy.org
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1 WORKSHOP <strong
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3 Content Workshop Book Embryolab A
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5 Hands-on Workshop 1 Fluor
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7 Programme 8:30 - 8:45 Pick- up fr
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Aneuploidy in male
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Spermatogenesis Hands-on Workshops,
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Paternally derived aneuploidies A 2
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Reciprocal Translocation The most f
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Interchromosomal Effect (ICE) Aneup
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Aneuploidy in male
Page 21 and 22: Sperm morphology Data regard<strong
Page 23 and 24: Infertile males (46,XY) Cryptorhidi
Page 25 and 26: DNA Fragmentation Index (DFI) Sever
Page 27 and 28: Occupational/Life style hazards Stu
Page 29 and 30: 41 Mechanisms again</strong
Page 31 and 32: Take home message At present, PGD i
Page 33 and 34: In summary • Multicolour
Page 35 and 36: FISH on Spermatozo
Page 37 and 38: FISH: What can it
Page 39 and 40: A DNA probe finds
Page 41 and 42: Hybridisation targ
Page 43 and 44: Sperm-FISH protoco
Page 45 and 46: 1. Preparation of sample Nuclear De
Page 47 and 48: 2. Co-Denaturation (target DNA & pr
Page 49 and 50: 5. Examine slides
Page 51 and 52: FISH Advantages )
Page 53 and 54: Figure 5. Model of chromosome organ
Page 55 and 56: 55 FISH technique:
Page 57 and 58: Tip for spreading
Page 59 and 60: Post Hybridization Wash The aim of
Page 61 and 62: Sperm FISH Externa
Page 63 and 64: Issue Date: 05/12/2012 Version: 1 R
Page 71: Our generous Sponsors 71
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WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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