WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm
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2. Co-Denaturati<strong>on</strong><br />
(target DNA & probe)<br />
Factors affect<str<strong>on</strong>g>in</str<strong>on</strong>g>g:<br />
1) Temperature<br />
2) Time<br />
*Formamide & pH (stable<br />
c<strong>on</strong>diti<strong>on</strong>s at co-denaturati<strong>on</strong>)<br />
25<br />
3. <str<strong>on</strong>g>Hybridisati<strong>on</strong></str<strong>on</strong>g><br />
Strigency: (tightly b<strong>on</strong>d): the degree to which<br />
the probe DNA will b<str<strong>on</strong>g>in</str<strong>on</strong>g>d and rema<str<strong>on</strong>g>in</str<strong>on</strong>g> bound<br />
to the target DNA (after hybridizati<strong>on</strong> and at<br />
Post-Hybridizati<strong>on</strong> washes)<br />
26
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