WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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<strong>FISH</strong> General Procedure 1. Preparation of the sample 2. Denaturation of the target DNA 3. Denaturation of the probe 4. <strong>Hybridisation</strong>–Post <strong>Hybridisation</strong> Wash 5. Fluorescence sta<strong>in</strong><strong>in</strong>g 6. Exam<strong>in</strong>e slides or store <strong>in</strong> the dark 11 Denaturation double-stranded DNA is made s<strong>in</strong>gle <strong>Hybridisation</strong> the formation of a duplex between two complementary sequences 12
<strong>Hybridisation</strong> target DNA denaturation hybridisation 13 14
Page 1 and 2: 1 WORKSHOP <strong
Page 3 and 4: 3 Content Workshop Book Embryolab A
Page 5 and 6: 5 Hands-on Workshop 1 Fluor
Page 7 and 8: 7 Programme 8:30 - 8:45 Pick- up fr
Page 9 and 10: Aneuploidy in male
Page 11 and 12: Spermatogenesis Hands-on Workshops,
Page 13 and 14: Paternally derived aneuploidies A 2
Page 15 and 16: Reciprocal Translocation The most f
Page 17 and 18: Interchromosomal Effect (ICE) Aneup
Page 19 and 20: Aneuploidy in male
Page 21 and 22: Sperm morphology Data regard<strong
Page 23 and 24: Infertile males (46,XY) Cryptorhidi
Page 25 and 26: DNA Fragmentation Index (DFI) Sever
Page 27 and 28: Occupational/Life style hazards Stu
Page 29 and 30: 41 Mechanisms again</strong
Page 31 and 32: Take home message At present, PGD i
Page 33 and 34: In summary • Multicolour
Page 35 and 36: FISH on Spermatozo
Page 37 and 38: FISH: What can it
Page 39: A DNA probe finds
Page 43 and 44: Sperm-FISH protoco
Page 45 and 46: 1. Preparation of sample Nuclear De
Page 47 and 48: 2. Co-Denaturation (target DNA & pr
Page 49 and 50: 5. Examine slides
Page 51 and 52: FISH Advantages )
Page 53 and 54: Figure 5. Model of chromosome organ
Page 55 and 56: 55 FISH technique:
Page 57 and 58: Tip for spreading
Page 59 and 60: Post Hybridization Wash The aim of
Page 61 and 62: Sperm FISH Externa
Page 63 and 64: Issue Date: 05/12/2012 Version: 1 R
Page 71 and 72: Our generous Sponsors 71
Page 73 and 74: 73 Certificate of attendance A Cert

WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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