WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm
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<str<strong>on</strong>g>FISH</str<strong>on</strong>g><br />
Results Interpretati<strong>on</strong><br />
<strong>Sperm</strong> <str<strong>on</strong>g>FISH</str<strong>on</strong>g> def<str<strong>on</strong>g>in</str<strong>on</strong>g>es the proporti<strong>on</strong> of aneuploidy present<br />
<str<strong>on</strong>g>in</str<strong>on</strong>g> the ejaculates of males with other (genetic/cl<str<strong>on</strong>g>in</str<strong>on</strong>g>ical)<br />
problems<br />
The goal: to identify “errors” and the “at-risk” males<br />
Quantitative vs Qualitative analyses<br />
“...<str<strong>on</strong>g>FISH</str<strong>on</strong>g> analysis of spermatozoa should be used as a tool of genetic screen<str<strong>on</strong>g>in</str<strong>on</strong>g>g <str<strong>on</strong>g>in</str<strong>on</strong>g><br />
<str<strong>on</strong>g>in</str<strong>on</strong>g>fertile patients. Significant differences <str<strong>on</strong>g>in</str<strong>on</strong>g> the rates of chromosome abnormalities<br />
with respect to c<strong>on</strong>trols should be taken <str<strong>on</strong>g>in</str<strong>on</strong>g>to c<strong>on</strong>siderati<strong>on</strong> regardless of the<br />
numerical value. When abnormal results are obta<str<strong>on</strong>g>in</str<strong>on</strong>g>ed, <str<strong>on</strong>g>in</str<strong>on</strong>g>dividuals should be<br />
identified as ‘‘at risk’’ and the couple should be advised about the available<br />
techniques of preimplantati<strong>on</strong> and prenatal genetic diagnosis”<br />
Sarrate et al, 2010<br />
35<br />
36
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