WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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Embryolab Academy Hands-on workshops <strong>in</strong> specialised ART laboratory techniques August 31 - September 5, 2014 Thessaloniki, Greece 70 # Name Title Institute e-mail Country 1 Ms. Elisabeth Foluke Philip Embryologist Georges Memorial Medical Centre folu25@yahoo.com Nigeria 2 Ms. Nicole Klauke Embryologist K<strong>in</strong>derwunschzentrum an der Gedächtniskirche / Berl<strong>in</strong> nicole-klauke@freenet.de Germany 3 Ms. Adedamilola Adesewa Atiba Embryologist NORDICA FERTILITY CENTER, LAGOS adedamilola.atiba@nordicalagos.org Nigeria 4 Ms. Styliani Sgoura Lab technician Embryolab s.sgoura@embryolab.eu Greece 5 Ms. Aimilia Vorniotaki Lab technician Embryolab a.vorniotaki@embryolab.eu Greece
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1 WORKSHOP <strong
Page 3 and 4:
3 Content Workshop Book Embryolab A
Page 5 and 6:
5 Hands-on Workshop 1 Fluor
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7 Programme 8:30 - 8:45 Pick- up fr
Page 9 and 10:
Aneuploidy in male
Page 11 and 12:
Spermatogenesis Hands-on Workshops,
Page 13 and 14:
Paternally derived aneuploidies A 2
Page 15 and 16:
Reciprocal Translocation The most f
Page 17 and 18:
Interchromosomal Effect (ICE) Aneup
Page 19 and 20: Aneuploidy in male
Page 21 and 22: Sperm morphology Data regard<strong
Page 23 and 24: Infertile males (46,XY) Cryptorhidi
Page 25 and 26: DNA Fragmentation Index (DFI) Sever
Page 27 and 28: Occupational/Life style hazards Stu
Page 29 and 30: 41 Mechanisms again</strong
Page 31 and 32: Take home message At present, PGD i
Page 33 and 34: In summary • Multicolour
Page 35 and 36: FISH on Spermatozo
Page 37 and 38: FISH: What can it
Page 39 and 40: A DNA probe finds
Page 41 and 42: Hybridisation targ
Page 43 and 44: Sperm-FISH protoco
Page 45 and 46: 1. Preparation of sample Nuclear De
Page 47 and 48: 2. Co-Denaturation (target DNA & pr
Page 49 and 50: 5. Examine slides
Page 51 and 52: FISH Advantages )
Page 53 and 54: Figure 5. Model of chromosome organ
Page 55 and 56: 55 FISH technique:
Page 57 and 58: Tip for spreading
Page 59 and 60: Post Hybridization Wash The aim of
Page 61 and 62: Sperm FISH Externa
Page 63 and 64: Issue Date: 05/12/2012 Version: 1 R
Page 69: Issue Date: 05/12/2012 Version: 1 R
Page 73 and 74: 73 Certificate of attendance A Cert
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WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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