WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
<str<strong>on</strong>g>FISH</str<strong>on</strong>g> Advantages<br />
) Less labor-<str<strong>on</strong>g>in</str<strong>on</strong>g>tensive method<br />
2) Used at uncultured cells<br />
3) Own c<strong>on</strong>trol<br />
4) No diluti<strong>on</strong> factor<br />
5) Small sample size<br />
6) Multiple target analysis<br />
7) Rather quick (possibly next day result)<br />
8)Easy to perform<br />
33<br />
<str<strong>on</strong>g>FISH</str<strong>on</strong>g> Disadvantages<br />
Can detect “known” or “suspected” abnormalities<br />
Not screen<str<strong>on</strong>g>in</str<strong>on</strong>g>g of chromosomal rearrangement<br />
No structural abnormalities<br />
No differentiati<strong>on</strong> between nullisomy & hybridisati<strong>on</strong><br />
failures<br />
Only 1-3 probe-signal / sperm head<br />
Cost<br />
Needs experienced technician<br />
Scor<str<strong>on</strong>g>in</str<strong>on</strong>g>g – Time c<strong>on</strong>sum<str<strong>on</strong>g>in</str<strong>on</strong>g>g<br />
(low aneuploidy rate/chromosome affected by sample's<br />
size)<br />
Automated <strong>Sperm</strong>-<str<strong>on</strong>g>FISH</str<strong>on</strong>g>?<br />
34
Change language