WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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Aneuploidy <strong>in</strong> male <strong>in</strong>fertile patients General population Males with abnormal karyotypes Infertile males with normal karyotypes Males exposed to certa<strong>in</strong> environmental/life style hazards Risk reduction Future Hands-on Workshops, Thessaloniki, 30.08 – 05.09 2014 47 Innovative techniques Development of more sensitive and <strong>in</strong>formative techniques: Further improvement of the <strong>FISH</strong> assay New or improved imag<strong>in</strong>g techniques Sperm RNA profil<strong>in</strong>g Array-based approaches and New Generation Sequenc<strong>in</strong>g Hands-on Workshops, Thessaloniki, 30.08 – 05.09 2014 48
In summary • Multicolour <strong>FISH</strong> technique: an effective tool • Certa<strong>in</strong> males are at risk • New techniques for risk assessment • New sperm process<strong>in</strong>g and selection techniques • More prospective randomised controlled studies Hands-on Workshops, Thessaloniki, 30.08 – 05.09 2014 49 Thank you! Any questions? 50
Page 1 and 2: 1 WORKSHOP <strong
Page 3 and 4: 3 Content Workshop Book Embryolab A
Page 5 and 6: 5 Hands-on Workshop 1 Fluor
Page 7 and 8: 7 Programme 8:30 - 8:45 Pick- up fr
Page 9 and 10: Aneuploidy in male
Page 11 and 12: Spermatogenesis Hands-on Workshops,
Page 13 and 14: Paternally derived aneuploidies A 2
Page 15 and 16: Reciprocal Translocation The most f
Page 17 and 18: Interchromosomal Effect (ICE) Aneup
Page 19 and 20: Aneuploidy in male
Page 21 and 22: Sperm morphology Data regard<strong
Page 23 and 24: Infertile males (46,XY) Cryptorhidi
Page 25 and 26: DNA Fragmentation Index (DFI) Sever
Page 27 and 28: Occupational/Life style hazards Stu
Page 29 and 30: 41 Mechanisms again</strong
Page 31: Take home message At present, PGD i
Page 35 and 36: FISH on Spermatozo
Page 37 and 38: FISH: What can it
Page 39 and 40: A DNA probe finds
Page 41 and 42: Hybridisation targ
Page 43 and 44: Sperm-FISH protoco
Page 45 and 46: 1. Preparation of sample Nuclear De
Page 47 and 48: 2. Co-Denaturation (target DNA & pr
Page 49 and 50: 5. Examine slides
Page 51 and 52: FISH Advantages )
Page 53 and 54: Figure 5. Model of chromosome organ
Page 55 and 56: 55 FISH technique:
Page 57 and 58: Tip for spreading
Page 59 and 60: Post Hybridization Wash The aim of
Page 61 and 62: Sperm FISH Externa
Page 63 and 64: Issue Date: 05/12/2012 Version: 1 R
Page 71 and 72: Our generous Sponsors 71
Page 73 and 74: 73 Certificate of attendance A Cert

WORKSHOP BOOK Fluorescent in Situ Hybridisation - FISH - on Human Sperm

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