Brucellosis 2003 proceedings - PHIDIAS

More documents

Recommendations

Info

Poster Session were isolated and typed in our laboratory by standard procedures and the remaining strains, eleven biovar 2 and one biovar 1, were kindly supplied and typed by C.M. Marín (Unidad de Sanidad Animal, Servicio de Investigacion Agrária, Zaragoza). Three reference strains, B. suis biovar 1/1330, biovar 2/Thomsen and biovar 3 (NCTC-PHLS, London) were used as controls. The results were analised by classical analysis of variance using the procedure GLM of SAS System. This analysis showed a significant difference amongst strains (p
Poster Session 53- ELISA AS AN ALTERNATIVE METHOD FOR THE DIAGNOSIS OF BRUCELLOSIS IN SHEEP. Jalali, A. 1 , Hemmatzadeh, F. 2 , Momtaz, H. 2 and Nilsson, E. 1 . (1) Svanova Biotech AB, Sweden. (2) Faculty of Veterinary Medicine, Tehran University, Iran. Brucellosis is an important zoonotic disease caused by the bacterium Brucella, affecting various animal species. Although many countries have succeeded to control this disease in cattle, brucellosis in small ruminants has been less favourable. However, due to the economical and health impacts of this issue, there is a need for easily standardized and performed tests for this purpose. Two antibody ELISA’s (SVANOVIR), one indirect and one competitive were evaluated on sheep sera. In the first stage 150 negative (Sweden) and 120 positive (Iran and Israel) samples were tested on both assays. The samples were characterized by serology and/or cultivation. The results were as follows: Assay Sensitivity Specificity Cut off Indirect ELISA 94,3 100 PP >= 15 Competitive ELISA 100 100 PI >= 30 To confirm validity of the chosen cut off, the trial was repeated on 1094 sheep samples from herds in Iran. All samples were tested with Rose Bengal (RBT), Wright and 2-ME according to standard procedures. Out of the 1094 sheep samples, 83 % were negative in all three conventional assays. Out of these only 5 % was positive in the C-ELISA whereas 41 % in the I-ELISA. Out of the 14 % tested positive in all three agglutination tests, 97 % was positive with the I-ELISA and 69% with the C-ELISA. Two percent of the total material showed discrepancies in the agglutination tests. However, in this group the specificity of the C-ELISA against 2-ME was 82%. The study shows that the ELISA tests could be suitable replacements for the conventional tests for screening as well as for confirmatory applications. 54- EVALUATION OF THE ELISA IN DIAGNOSIS OF BRUCELLOSIS IN PIGS. K. Szulowski, W. Iwaniak, J. Pilaszek. Department of Microbiology, National Veterinary Research Institute in Pulawy, Poland. According to Manual of Standards for Diagnostic Tests and Vaccines the serological methods recommended in diagnosis of brucellosis in pigs are ELISA, BBAT and FPA. The aim of the studies was to evaluate the properties of the indirect ELISA (I-ELISA) used in diagnosis of swine brucellosis in Poland. In the test the microplates coated with the “smooth” lipopolysaccharide (S-LPS) obtained from the strain S19 of Brucella abortus, the conjugate of anti-swine immunoglobulins with horseradish peroxidase and ABTS with H 2 O 2 as the substrate, were used. The controls of the ELISA kit consisted of: strong positive swine serum (S++), weak positive serum (S+) and negative serum (S-) prepared respectively on the base of sera from pigs coming from Brucella infected herds and pigs free of brucellosis. In the examination 105 sera from pigs from 3 infected herds, 92895 sera from healthy pigs monitored for brucellosis from territory of Poland and 3662 sera from imported pigs were used. Besides ELISA the traditional methods such as RBT, CFT and additionally for some samples, SAT and 2-ME were used. Of pigs from infected herds 102 sera reacted positively in the ELISA, whereas 95 sera were positive in the RBT and 98 in the CFT. In the screening surveys only 11 positive results were obtained in Brucellosis 2003 International Research Conference 119
Page 1:
Brucellosis 2003 International Rese
Page 5:
Welcome As Professor Paul Nicoletti
Page 9:
Instructions to presenters KEYNOTE
Page 13:
Scientific Program
Page 16 and 17:
Scientific Program - Monday, Septem
Page 18 and 19:
Scientific Program - Monday, Septem
Page 20 and 21:
Scientific Program - Tuesday, Septe
Page 22 and 23:
Scientific Program - Wednesday, Sep
Page 24 and 25:
Scientific Program - Poster Session
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 35:
Abstracts
Page 38 and 39:
Keynote Lectures while B. ovis and
Page 40 and 41:
Keynote Lectures BRUCELLOSIS IN WIL
Page 42 and 43:
Keynote Lectures CLASSICAL AND NEW
Page 44 and 45:
Keynote Lectures COMPARISON OF THE
Page 46 and 47:
Keynote Lectures projects (localizo
Page 48 and 49:
Short Oral Communications Epidemiol
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Short Oral Communications Human bru
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Short Oral Communications Diagnosis
Page 62 and 63:
Page 64 and 65:
Page 66 and 67: Short Oral Communications Immunolog
Page 76 and 77: Short Oral Communications Vaccines
Page 84 and 85: Short Oral Communications Taxonomy
Page 86 and 87: Short Oral Communications Taxonomy
Page 88 and 89: Poster Session and all male animals
Page 90 and 91: Poster Session 6- SEROLOGICAL INCID
Page 92 and 93: Poster Session situation of a long-
Page 94 and 95: Poster Session 14- HIGH PREVALENCE
Page 96 and 97: Poster Session samples tested posit
Page 98 and 99: Poster Session time. To confirm the
Page 100 and 101: Poster Session 25- PRESENTATION OF
Page 102 and 103: Poster Session specific diagnosis i
Page 104 and 105: Poster Session Urinalysis was notab
Page 106 and 107: Poster Session sera were positive w
Page 108 and 109: Poster Session controversial. While
Page 110 and 111: Poster Session 41- RAPID DETECTION
Page 112 and 113: Poster Session 44- DEVELOPMENT AND
Page 114 and 115: Poster Session Brucella ovis, Bruce
Page 118 and 119: Poster Session the ELISA, whereas 7
Page 120 and 121: Poster Session agglutination (SAT),
Page 122 and 123: Poster Session investigations in ap
Page 124 and 125: Poster Session 65- PHAGOCYTOSIS AND
Page 126 and 127: Poster Session 68- DOES OXYGEN TENS
Page 128 and 129: Poster Session 72- IMPLICATION OF F
Page 130 and 131: Poster Session 76- THE AQUAPORIN GE
Page 132 and 133: Poster Session adaptation to starva
Page 134 and 135: Poster Session constituents that ar
Page 136 and 137: Poster Session and revaccinated ani
Page 138 and 139: Poster Session weeks after infectio
Page 140 and 141: Poster Session melitensis wild type
Page 142 and 143: Poster Session indicated that HS en
Page 144 and 145: Poster Session This study aimed to
Page 146 and 147: Poster Session definition. Reviewin
Page 148 and 149: Poster Session ApaI+0/MseI+G) were
Page 150 and 151: Poster Session 109- COMPARATIVE PRO
Page 152 and 153: Poster Session enterobactin. At the
Page 154 and 155: Poster Session apparent differences
Page 157 and 158: Author index A Abalos, P.----------
Page 159 and 160: Author index GonzálezCarreró, M I
Page 161 and 162: Author index Q Qasem, J A.---------
Page 163 and 164: List of participants ABERNETHY, DAR
Page 165 and 166: List of participants CARNERO OJEA,
Page 167 and 168:
List of participants Fax: 301 319 9
Page 169 and 170:
List of participants Phone: 1 97 98
Page 171 and 172:
List of participants IOANNOU, IOANN
Page 173 and 174:
List of participants LÓPEZ-GOÑI,
Page 175 and 176:
List of participants NEUBAUER, HEIN
Page 177 and 178:
List of participants E-mail: rajash
Page 179 and 180:
List of participants SUAREZ-GUEMES,
Page 181:
Sponsors Brucellosis 2003 Internati
show all

Brucellosis 2003 proceedings - PHIDIAS

Create successful ePaper yourself

Delete template?

Save as template?