Brucellosis 2003 proceedings - PHIDIAS

Short Oral Communications
Diagnosis in animal brucellosis
DO1- SEROLOGICAL RESPONSE OF YOUNG AND ADULT SHEEP TO
CONJUCTIVAL VACCINATION WITH REV-1 VACCINE ASSESSED WITH THE
CLASSICAL AND RECENT TESTS.
A. Stournara 1 , A. Minas 1 , E. Bourtzi-Chatzopoulou 2 , G. Koptopoulos 2 , E. Petridou 2 , K. Sarris 2 . (1)
Veterinary Laboratory of Larissa, National Reference Laboratory of Brucellosis, 411 10, Larissa,
Greece. (2) Laboratory of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine,
Aristotelian University of Thessaloniki, 54 124, Thessaloniki, Greece.
The serological response of young and adult sheep to conjuctival vaccination
with REV-1 vaccine was assessed with the use of classical and new serological tests.
One hundred and six (106) adult sheep and forty-nine (49) lambs 3-6 months old,
reared in a brucellosis officially free flock, were used for this purpose. A normal dose
(1?10 9 cfu) of REV-1 vaccine was administered conjunctivaly to all animals. Prior to
vaccination the animals were bled and the sera collected were tested with the
serological tests approved for the diagnosis of ovine and caprine brucellosis as Rose
Bengal test (RBT) and Complement Fixation Test (CFT) ( Directive 91/68 EEC) . The
sera were tested also with the most sensitive modified Rose Bengal test (m-RBT) (
Blasco, 1994 , OIE, 2002) and new tests as, indirect Elisa (i-ELISA) , (Jimenez de
Bagues, 1991), competitive ELISA (c-ELISA) (Nielsen, 1995) and Flourescence
Polarization Assay (FPA) (Nielsen, 1996). The results obtained by all tests performed
were negative. The animals were bled at day 21, 42, 63, 91, 125, 159, 223 and 330
post vaccination and the sera collected were tested with the tests mentioned above.
The performed tests revealed that three weeks (21 days) post vaccination the
seropositive adult animals to RBT, m-RBT, CFT, c-ELISA, i-ELISA and FPA
represented the 100%, 100%, 98%, 88,6%, 87% and 83% of vaccinated adult
animals respectively, while the seropositive lambs to these tests represented the
100%, 100%, 89,7%, 79,59%, 18,36 and 18,36% of vaccinated young animals
respectively. Forty seven weeks (330 days) post vaccination the seropositive adult
animals to RBT, m-RBT, CFT, c-ELISA, i-ELISA and FPA represented the 3,77%,
11,32%, 1,88%, 2,83%, 33% and 2,83% of vaccinated adult animals respectively . At
the same time seropositive lambs that represented the 2,04% of vaccinated young
animals were found with m-RBT, while the results obtained from all the other tests
were negative.
DO2- EVALUATION OF THE COMPETITIVE ENZYME IMMUNOASSAY AND
POLARIZED FLUORESCENCE ASSAY FOR BRUCELLOSIS DIAGNOSIS IN
GOATS FROM NORTHERN MÉXICO.
C. Ramirez-Pfeiffer 1 , A. C. Snydelaar-Hardwicke 2 , F. Acosta-Martínez 2 , J. Vázquez-Villanueva 2 , E.
Luna-Martínez 3 , R. Gómez-Flores 4 , K. Nielsen 5 . (1) Instituto Nacional de Investigaciones Forestales y
Agropecuarias, CIRNE, C. E. Aldama, México. (2) Universidad Autónoma de Tamaulipas, Fac. Med.
Veterinaria y Zoot., México. (3) Secretaría de Agricultura Ganadería, Desarrollo Rural, Pesca y
Alimentación, SENASICA, México. (4) Universidad Autónoma de Nuevo León. Fac. Ciencias
Biológicas, México. (5)Canadian Food Inspection Agency. Animal Disease Research Institute,
Canada.
A preliminary study to determine the effectiveness of the fluorescence
polarized assay (FPA) and competitive enzyme immunoassay (CELISA) in detecting
negative serums was performed. Results were compared with those obtained with
the card test using 3% of antigen concentration. Procedures and reagents used were
Brucellosis 2003 International Research Conference
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