Brucellosis 2003 proceedings - PHIDIAS

Short Oral Communications
Diagnosis in animal brucellosis
Ref. 1 : B. Garin-Bastuji, R. Pouillot, C. Cau, P. Pourquier, L. Schalch, P. Very :
Evaluation of two commercially available iELISA kits for the diagnosis of Bovine
Brucellosis on pools of 10 sera. Comparison with the RB, CF and iELISA performed
on individual sera.
Ref. 2 : Axel Cloeckaert, Sylvie Baucheron, Nieves Vizcaino, Michel s. Zygmunt :
Use of Recombinant BP26 Protein in Serological Diagnosis of Brucella melitensis
Infection in Sheep.
Ref. 3 : EEC Directive 64.432
DO4- DIFFERENCES IN CELLULAR IMMUNE RESPONSES OF PIGS
EXPERIMENTALLY INFECTED WITH Brucella suis AND Yersinia enterocolitica
SEROTYPE O:9.
G.Jungersen and U.Riber. Danish Veterinary Institute, Copenhagen, Denmark.
Due to almost identical LPS O-antigens, infections with Yersinia enterocolitica
serotype O:9 (YeO:9) cause false positive reactions in serological tests for Brucella.
As LPS are strong inducers of humoral immune responses, cellular immune
responses to non-LPS antigens prepared from rough Brucella melitensis
(Brucellergene®) was hypothesized to separate these two very different infections in
pigs. Ten young pigs were experimentally inoculated in the eye sac with 2×10 7 cfu of
Brucella suis biovar 2 and cellular immune responses were followed for 20 weeks. As
evidenced by antibody responses in serum and cultivation of tissue samples at
slaughter 7 pigs were successfully infected. Immune responses were compared with
the responses in age matched pigs, experimentally infected per os with different
doses of YeO:9.
Whole blood interferon-gamma (IFN-γ) test (stimulated with dialysed
Brucellergene®, Yersinia secretory proteins (yop), positive and negative controls)
revealed high IFN-γ levels, in response to Brucellergene® in Brucella infected pigs
but not in YeO:9 infected or control pigs. In comparison, IFN-γ responses to yop and
PBS were low in all groups of pigs irrespective of type of infection. The specificity of
the cellular immune responses was confirmed by positive skin test in Brucella, but not
Yersinia, infected pigs at different times after inoculation. Intracellular FACS staining
for IFN-γ and BrdU incorporation showed CD4 + and CD8 low T cells were main
producers of IFN-γ and the predominant proliferating cell subset following
Brucellergene® stimulation of Brucella infected pigs. In conclusion, cellular mediated
immune responses to non-LPS Brucella antigens were both specific and sensitive in
discriminating subclinical experimental infections with B. suis from infections with
YeO:9.
DO5- BRUCELLOSIS IN THE NETHERLANDS: THE DEVELOPMENT OF
Brucella-SPECIFIC SEROLOGICAL TESTS.
B. Kooi, L. Mastebroek, P. Willemsen, D. Bakker and F. van Zijderveld. Central Institute for Animal
Disease Control (CIDC) Lelystad, The Netherlands.
Since 1999 the Netherlands gained the status of an officially brucellosis free
(OBF) member state on the basis of directive 64/432/EEC. Since then, 20% of the
Brucellosis 2003 International Research Conference
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