Brucellosis 2003 proceedings - PHIDIAS

Poster Session
299 (43, 1%) with positive results in CFT and 488 (64, 6%) in RBT and with doubtful
results: 88 (12, 6%) in CFT and 19 (2, 74%) in RBT. Though the positive results of
both test shown to be comparable, however CFT it was proved to be more conclusive
from a diagnostically point of view, because of the easy interpretation and by titration
of specific antibody we may obtain a diagram of the antibody dynamic since evolution
of the disease and also we could evaluate the efficiency of the prescribed treatment.
57- SEROLOGICAL SURVEYS OF DOGS FOR Brucella canis INFECTIONS IN
POLAND.
W. Iwaniak, K. Szulowski, J. Pilaszek. Department of Microbiology, National Veterinary Research
Institute in Pulawy, Poland.
Brucellosis of dogs is infectious disease caused by Brucella canis. In
serological diagnosis of brucellosis in dogs the following tests are used: RSAT, 2ME -
RSAT, TAT, 2ME-TAT, AGID and ELISA. The aim of the research was evaluation of
diagnostic properties of the tests used in recognizing of brucellosis in dogs in Poland.
In investigations 765 sera originating from dogs were used – in this number 3
diagnostic sera, 42 sera from dogs naturally infected (obtained from USA) and 720
sera from dogs from Poland (17 of them originated from dogs with clinical symptoms,
which could indicate brucellosis). Antigenic preparations were made in own range
from "M-" (less mucoid variant) strain B. canis. The commercial antigens for RSAT
and AGID – both made in USA, were also used in the research. When examining 42
sera from naturally infected dogs, 41 reacted positively in RSAT and 2ME-RSAT, 39
in TAT and 37 in 2ME-TAT. In AGID positively reacted 34 sera and 41 in ELISA.
What concerns dogs` sera from Poland – 9 of them reacted positively in 2ME-RSAT,
5 in 2ME-TAT and AGID and 15 in ELISA. Of sera from dogs with symptoms which
could indicate brucellosis, 1 serum reacted positively in 2ME-TAT and AGID whereas
3 in ELISA. The investigations showed a high correlation between the results
obtained by the own and commercial antigens. The highest sensitivity showed RSAT
and then in succession TAT, ELISA and AGID. The results of investigations
confirmed a good quality of home made antigens. The tests utilizing these antigens
can be used in serological diagnosis of brucellosis in dogs caused by B. canis. The
examination enabled to ascertain the native reservoir of B. canis.
58- BRUCELLOSIS IN PINNIPEDS FROM ARGENTINA.
F. Capellino 1 , S. Conde 1 , M. Uhart 2 , D. Albareda 3 , L. Samartino 1 . (1) INTA CCVyA Castelar, Inst.
Patobiología, Bacteriología, Bs. As., Argentina, (2) Wildlife Conservation Society, (3) Acuario de
Buenos Aires, Argentina.
Brucellosis in marine mammals has been recently described in several parts of
the world (Handbook of Marine Mammal Medicine, 2001, 4:6). We studied the levels
of antibody induced by Brucella sp in pinnipeds from Argentina’s extensive coastline.
We also evaluated the performance of conventional and non-conventional tests used
for brucellosis detection. One hundred and sixty samples were analyzed. Twelve
from fur seals (Arctocephalus australis), 4 from southern sea lions (Otaria flavescens)
and 144 from southern elephant seals (Mirounga leonina) were tested. Seven
different serological tests were evaluated: Buffered Plate Antigen (BPA), Wright
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