Brucellosis 2003 proceedings - PHIDIAS

Poster Session
and all male animals. Positive cattle in RB were tested also in c and Ab ELISA, other
positive animals in RB were tested in cELISA. From all tested cattle in year 2002, 72
animals were positive in RB test. None of them were positive in CFT. In Ab ELISA
were positive 3 samples. In cELISA 3 animals were positive but not the same as in
Ab ELISA. Only 3 tested pigs were positive in RB and 1 he-goat which were negative
in CFT and in cELISA. We shall satisfy the requirements to qualify Slovenia as
country free from bovine brucellosis with testing of same number of cattle samples in
2003 as in 2002.
3- PREVALENCE OF BOVINE BRUCELLOSIS IN HERDS SUPPLYING MILK TO
LOCAL MARKETS IN GUINEA, THE GAMBIA AND SENEGAL AND
ASSOCIATED PUBLIC HEALTH RISK.
F. Unger 1 , A. Goumou 2 , B. Diallo 2 , M. Konte 4 , M. Hempen 1 , S. Münstermann 1 and K. H. Zessin 3 . (1)
International Trypanotolerance Centre, Banjul, The Gambia. (2) Departement National d´ Èlevage,
Conakry, Guinea. (3) Freie Universität Berlin, Department for International Animal Health, Berlin,
Germany. (4) Institut Senegalais Recherche Agricole, Dakar, Senegal.
Zoonoses are of particular public health importance in societies that live
closely together with their livestock. Brucellosis, in particular, is easily transmitted via
raw milk, the predominant consumption pattern in West Africa. In order to assess the
importance of brucellosis, 2368 cattle in selected Districts supplying milk to the local
markets in Guinea (Dubreka, 17 farms and Boke, 19 farms), The Gambia (Central
River Division, 20 farms) and Senegal (Bassin Arachidier, 30 farms) were enrolled in
a bovine brucellosis serological herd screening survey (May 2001 - October 2002).
All serum samples were screened using Rose Bengal Plate Test (RBPT) and
Complement Fixation Test (CFT) as confirmatory test for RBPT positive samples.
Bulk milk samples were collected in Guinea and Senegal and subjected to Milk Ring
Test (MRT) or ELISA test (BOMMELI®). Farmers were interviewed on their
knowledge and awareness of brucellosis.
Results indicate different epidemiological situations for brucellosis in the three
countries. Highest mean prevalences of all animals per area surveyed were found in
Guinea with 12.7 % in Dubreka and 6.3 % in Boké. These values were considerably
lower in The Gambia (1.1 %) and in Senegal (0.6 %). Highest herd prevalences were
also demonstrated in Guinea with 16 of 17 herds (Dubreka) and 14 of 19 herds
(Boké) testing at least one animal positive. In contrast, 2 of 20 herds were found
positive in The Gambia and 3 of 30 in Senegal. Herds tested positive in their bulk
milk samples (MRT or ELISA) were always amongst those serologically positive. The
knowledge of farmers on the zoonotic character was generally poor. Milk was
consumed nearly 100 % non-heated. High prevalences in cattle in Guinea prompted
testing of volunteers (herdsmen and herd owners) in the same Districts with seven of
20 people diagnosed positive. This result underlines the high public health risk
associated to bovine brucellosis in Guinea.
4- SEROLOGICAL SURVEY ON BRUCELLOSIS IN HUMAN, SHEEP AND GOATS
IN CENTRAL PART OF IRAN.
T. Zahraei – Salehi 1 and A. Haseli Abarghoei 2 . (1) Department of Microbiology & Immunology, Faculty
of Veterinary Medicine, Tehran University, Tehran, Iran. (2) Graduated from the Faculty of Veterinary
Medicine, Tehran University, Tehran, Iran.
90
Brucellosis 2003 International Research Conference