Brucellosis 2003 proceedings - PHIDIAS

Poster Session
samples tested positive for brucellosis (B. suis). In order to investigate brucellosis in
further detail, the spleen and the reproductive organs (accessory sexual glands and
the uterus respectively) have been collected in the second hunting season. Disease
prevalence or freedom from disease respectively will be discussed for brucellosis
among wild boars in Switzerland.
18- SEROLOGICAL SURVEY FOR REINDEER (Rangifer tarandus tarandus)
BRUCELLOSIS IN FINLAND.
E. Seuna 1 and V. Hirvelä-Koski 2 . Department of Bacteriology, Helsinki (1), Oulu Regional Unit (2),
National Veterinary and Food Research Institute EELA, Finland.
Rangiferine brucellosis caused by Brucella suis biotype 4 has been reported in
circumpolar areas, North America and Russia, but not in Scandinavia. No information
is available of the occurrence of rangiferine brucellosis in Russian areas adjacent to
Finland. There are 57 reindeer herding co-operative districts in the northern part of
Finland. Serological surveys were conducted in 1997-1998 and 2001-2002 to study
the occurrence of Brucella antibodies in semi-domesticated reindeer. The total
number of reindeer in 1997-1998 was about 286 000, while in 2001-2002 the figure
was 297 000. 2 307 serum samples from 14 districts and 1 676 samples from 39 of
the 57 districts were collected at slaughter in 1997-1998 and 2001-2002,
respectively. The samples were tested for Brucella antibodies using Rose Bengal
Plate Agglutination Test in both surveys. No seropositive samples were detected.
The negative results indicate that the prevalence of brucellosis was less than 0.13%
in 1997-1998, and less than 0.18% in 2001-2002, with 95% confidence.
19- PREVALENCE OF BRUCELLOSIS IN WILD SWINE (Sus scrofa) IN THE
REPUBLIC OF CROATIA.
Z.Cvetnic 1 , J. Toncic 2 , S. Spicic 1 , M. Lojkic 1 , S. Terzic 1 , L. Jemersic 1 , A. Humski 1 , M. Ocepek 3 and
B.Krt 3 (1) Croatian Veterinary Institute Zagreb, Croatia. (2) Institute of Forestly, Jastrebarsko, Croatia.
(3) Veterinary Faculty, Ljubljana, Slovenia.
During 2001 and 2002 on seven localities in Croatia a survey on the
prevalence of brucellosis in wild swine was carried out. The survey included 271
(52.7%) female and 243 (47.3%) male animals between 7 months and 4 years of
age. On that occasion 514 blood samples of wild swine were serologically analysed.
For serological analysis Rose Bengal test (RBT), slow agglutination test (SAT),
complement fixation test (CFT) and indirect enzyme immunoassay (iELISA) were
used. In all of the wild swine from all of the localites investigated positive reactions to
brucellosis were established. Most of the positive reactions were established by
iELISA (13.6%), then by RBT (11.5%), CFT (10.5%) and SAT (8.9%). The samples of
134 testes, 39 uteri and 5 fetuses of piglets were analysed bacteriologically.
Brucellae spp. were isolated from 18 (10.1%9 samples that were collected from all of
the localities investigated. The results of PCR using BRU-UP and BRU-LOW primers
confirmed that all of the isolates were from Brucella spp. Genus, whereas, for the
analysis of fragment IS 711 DNA multiplication using two specific primers (IS 711 and
B. suis - specific primer) was carried out. In all the isolates investigated and in the
standard cultures of B. suis biovar 1 (1330) and B. suis biovar 2 the product of
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Brucellosis 2003 International Research Conference