Brucellosis 2003 proceedings - PHIDIAS

Poster Session
challenged Balb/c mice. Vaccines based upon omp25 were shown to be at least as
protective as Rev1 in challenge studies with B. melitensis 16M.
97- IMMUNOGENICITY AND PROTECTIVE EFFICACY OF 5 NOVEL DNA
VACCINES IN THE BALB/c MOUSE.
Nicky Commander, Rachel Ives. Veterinary Laboratories Agency, Woodham Lane, New Haw,
Addlestone, Surrey, KT15 3NB, UK.
The development and in-vitro assessment of DNA vaccines encoding the
genes omp25, FliC, FrpB, AcvB, and invasion protein B is described in
accompanying posters 1 and 2. The immunogenicity and protective efficacy of these
candidates was assessed in the Balb/c mouse. Each candidate was shown to elicit a
Brucella specific immune response. Serological assessment indicated a bias from all
candidates toward IgG 2a isotype antibody production, suggesting a Th1 mediated
immune response. Specific cytokine production was measured through RT-PCR and
ELISA of cell lysates or supernatants from specific antigen stimulated splenocytes.
IFN-γ and IL-4 were detected up to 12 weeks post vaccination. In protection studies,
vaccinated mice were challenged with B. melitensis 16M. Mice receiving the DNA
vaccines based upon invasion protein B and omp25 were able to control infection to
a similar level to those receiving vaccination with the live attenuated vaccine Rev1.
Here we describe the immunological findings and their relation to the protection
results.
98- THE SELECTION OF POTENTIAL PROTECTIVE ANTIGENS FOR
DEVELOPMENT OF SUB-UNIT VACCINES AGAINST BRUCELLOSIS.
Nicky Commander, Pauline Groussaud, James Tucker. Veterinary Laboratories Agency, Woodham
Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK.
Identification of protective antigens is the first step in rational vaccine design.
The recent completion of the Brucella spp. genomes and the availability of numerous
techniques for in depth in-silico analysis, has enabled us to identify a selection of
potentially important antigens for investigation as vaccine candidates. Homologies to
known protective antigens, structural information and putative T and B cell epitopes
were assessed through bioinformatics approaches. RT-PCR techniques were then
used to determine the expression of these candidate genes from B. melitensis 16M,
cultured under various conditions. This poster describes the first step toward the
development of rationally designed DNA vaccines through this selection and
elimination of putative vaccine candidates.
99- THE EFFECTS OF VARIOUS FACTORS ON THE VIABILITY OF Brucella
abortus STRAIN 19 VACCINE.
Enaan M. El Sanousi 1 & S.M. El Sanosi 2 . (1) Central Veterinary Research Laboratories Center
Brucella Department. Animals Resources Research Corporation. Ministry of Science and Technology.
Sudan. (2) Dean of the Faculty of Veterinary Science, University of Khartoum. Sudan.
Brucellosis 2003 International Research Conference
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