Brucellosis 2003 proceedings - PHIDIAS

Short Oral Communications
Diagnosis in animal brucellosis
RB 1:1 were considered RB 1:1 positive and samples containing 20 ICFT units/ml
were considered CFT confirmed positive. Three hundred fifteen flocks (315) showed
one or more animals reacting with RB 1:3 including one hundred thirteen (113) flocks
with one or more CFT confirmed positive animals. From the three hundred fifteen
(315) RB 1:3 positive flocks, ninety-four (94) showed one or more animals reacting
with RB 1:1 including forty eight (48) flocks with one or more animals confirmed
positive with CFT. Among the two hundred twenty one (221) flocks that were
negative to RB 1:1 and positive to RB 1:3, sixty five (65) flocks included one or more
animals confirmed positive with CFT. In this trial, RB 1:3 and RB 1:1 detected from
the population examined, in series with CFT, 5,1% and 2,16% positive flocks
respectively while a proportion of 9,1% and 2,076% of the flocks reacting to RB 1:3
and RB 1:1 respectively, were not confirmed as positive. Also, the CFT confirmed as
positive 35,8% of the flocks having animals with one or more reactions to RB 1:3.
This proportions for RB 1:1 reached 51%.
DO7- DIAGNOSING OF OVINE AND CAPRINE BRUCELLOSIS TROUGH THE
SERUM AND MILK ELISA - PRELIMINARY TEST VALIDATION.
Bosnakovski J., Mitrov D., Naletoski I. Veterinary Institute, Skopje, Republic of Macedonia.
With ELISA methods it is possible to examine individual serum/milk samples
(like confirmatory test) and pooled milk samples (like screening test). ELISA for
pooled milk samples is useful for examination of flocks from 200 cows (Brucella
bang), 100 sheep or 100 goats (Br. melitensis). The approach for standardization of
tests is going trough the Bommeli ELISA-BESW (Brucella bang) standard and our
institute (MKD) working standard (positive serum and milk, based on Brucella
melitensis antigen). The specificity and sensitivity of the Chekit-Brucellotest ELISA
and our institute (MKD) ELISA has been evaluated by the Department for Cattle
Diseases at the Veterinary Institute, Skopje with routine samples serum/milk obtained
parallel from brucellosis free herds and from infected herds, respectively. A total of
396 negative and 755 positive samples of both, sera and milk were included in the
examination. The samples used in the preliminary validation were previously tested
on RBT and CFT and were both negative in negative samples, and both positive in
positive samples (combination of RBT and CFT used as a "golden standard").
The obtained specificity of the serum ELISA was 99.7 (at cut-off of 30%PP
and 15% PP for Bommeli and MKD ELISA, respectively). The sensitivities of serum
ELISAs at the same cut-off were 98.5% for Bommeli and 96.7% for the MKD test.
Parallel milk samples from the same animals showed same specificity of 99.7% in
both ELISA tests, but using different cut-offs of 30% for Bommeli test and 25% for the
MKD test. The sensitivity of the milk ELISAs were 94.6% for the Bommeli test and
95.9% for the MKD test. Using ELISA technique it is possible to diagnose Brucellosis
fast, safe exact and cheap.
Brucellosis 2003 International Research Conference
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