Brucellosis 2003 proceedings - PHIDIAS

More documents

Recommendations

Info

Short Oral Communications Diagnosis in animal brucellosis DO8- THE USE OF FTA CARDS IN MOLECULAR DIAGNOSTIC TECHNIQUES FOR BRUCELLOSIS. Betsy J. Bricker 1 , Steven C. Olsen 1 and Darla R. Ewalt 2 . (1) USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA, USA. (2) USDA, Animal and Plant Health and Inspection Service, Veterinary Services, National Veterinary Services Laboratories, Ames, IA, USA. There has recently been increased interest in the development of molecularbased diagnostic techniques for the identification and fingerprinting of Brucella isolates. One impediment for this technology is sample preparation, since most methods (especially PCR-based methods) are very sensitive to inhibitors from blood, milk, tissues, or foods. Culturing the bacteria is an effective way to eliminate inhibitors, but it is time-consuming. Alternatively, DNA extraction and purification from the inhibitors is also time-consuming and may lead to cross-contamination of samples. We describe the use of commercially available FTA cards as a rapid method for preparing Brucella DNA for PCR and other enzymatic reactions. These cards, commonly used for forensic analysis of blood and buccal samples, consist of filter paper impregnated with solubilizers and detergents. When a sample is spotted on the card the cells or bacteria are lysed and the DNA binds to the filter. Contaminants and inhibitors are easily removed by a few wash steps. We demonstrate that the treated samples are readily amplified by PCR without any significant loss of sensitivity. DO9- MARINE MAMMAL BRUCELLOSIS IN CANADA 1995-2003. O. Nielsen 1 , K. Nielsen 2 , D. Ewalt 3 , B. Dunn 1 and S. Raverty 4 . (1) Department of Fisheries and Oceans Canada, Winnipeg, Manitoba, Canada. (2) Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada. (3) United States Department of Agriculture, National Veterinary Services Laboratories, Ames, Iowa, USA. (4) Animal Health Centre, British Columbia Ministry of Agriculture and Food, Abbotsford, British Columbia, Canada. Since the discovery of marine mammal forms of Brucella in 1994 there have been numerous surveys to identify the strains involved, the hosts they infect, and their geographical distribution. Most of what is known about marine mammal Brucellae has been obtained from investigations of stranded or captive animals and a few serological surveys of wild animals. There has been some recent progress, as evidenced by reports that some cetacean stranding events are associated with meningoencephalitis caused by Brucella, accounts of reproductive failure in dolphins and baleen whales due to Brucella and that at least two human, community-acquired cases of neurobrucellosis resulted from Brucella infection that is believed to have arisen from marine mammal sources. A marine mammal sampling program of hunterkilled (and presumably healthy animals) and post mortem examination of stranded marine mammals has been on going in arctic and Pacific regions of Canada since the early 1980's and late 1990’s respectively, and has provided some valuable insights regarding the epidemiology of marine mammal Brucellae. Specifically, serological testing using a Brucella-specific C-ELISA assay method has documented Brucella exposure in both phocid and cetacean species. Results from these investigations indicate that marine mammal brucellosis is widespread in Canadian waters affecting virtually all species tested. Prevalence is high and in some areas may approach 50%. Twelve isolations have been made from various species of 64 Brucellosis 2003 International Research Conference
Short Oral Communications Diagnosis in animal brucellosis seals and beluga (Delphinapterus leucas) throughout Canada and all appear identical based upon their biochemical testing and characterization. The impact of brucellosis on endangered marine mammals is largely unknown but evidence is accumulating that it may be contributing to the decline of some fragile stocks. Canadian examples will be presented. Brucellosis 2003 International Research Conference 65
Page 1:
Brucellosis 2003 International Rese
Page 5:
Welcome As Professor Paul Nicoletti
Page 9:
Instructions to presenters KEYNOTE
Page 13: Scientific Program
Page 16 and 17: Scientific Program - Monday, Septem
Page 18 and 19: Scientific Program - Monday, Septem
Page 20 and 21: Scientific Program - Tuesday, Septe
Page 22 and 23: Scientific Program - Wednesday, Sep
Page 24 and 25: Scientific Program - Poster Session
Page 35: Abstracts
Page 38 and 39: Keynote Lectures while B. ovis and
Page 40 and 41: Keynote Lectures BRUCELLOSIS IN WIL
Page 42 and 43: Keynote Lectures CLASSICAL AND NEW
Page 44 and 45: Keynote Lectures COMPARISON OF THE
Page 46 and 47: Keynote Lectures projects (localizo
Page 48 and 49: Short Oral Communications Epidemiol
Page 54 and 55: Short Oral Communications Human bru
Page 60 and 61: Short Oral Communications Diagnosis
Page 62 and 63: Short Oral Communications Diagnosis
Page 66 and 67: Short Oral Communications Immunolog
Page 76 and 77: Short Oral Communications Vaccines
Page 84 and 85: Short Oral Communications Taxonomy
Page 86 and 87: Short Oral Communications Taxonomy
Page 88 and 89: Poster Session and all male animals
Page 90 and 91: Poster Session 6- SEROLOGICAL INCID
Page 92 and 93: Poster Session situation of a long-
Page 94 and 95: Poster Session 14- HIGH PREVALENCE
Page 96 and 97: Poster Session samples tested posit
Page 98 and 99: Poster Session time. To confirm the
Page 100 and 101: Poster Session 25- PRESENTATION OF
Page 102 and 103: Poster Session specific diagnosis i
Page 104 and 105: Poster Session Urinalysis was notab
Page 106 and 107: Poster Session sera were positive w
Page 108 and 109: Poster Session controversial. While
Page 110 and 111: Poster Session 41- RAPID DETECTION
Page 112 and 113: Poster Session 44- DEVELOPMENT AND
Page 114 and 115:
Poster Session Brucella ovis, Bruce
Page 116 and 117:
Poster Session were isolated and ty
Page 118 and 119:
Poster Session the ELISA, whereas 7
Page 120 and 121:
Poster Session agglutination (SAT),
Page 122 and 123:
Poster Session investigations in ap
Page 124 and 125:
Poster Session 65- PHAGOCYTOSIS AND
Page 126 and 127:
Poster Session 68- DOES OXYGEN TENS
Page 128 and 129:
Poster Session 72- IMPLICATION OF F
Page 130 and 131:
Poster Session 76- THE AQUAPORIN GE
Page 132 and 133:
Poster Session adaptation to starva
Page 134 and 135:
Poster Session constituents that ar
Page 136 and 137:
Poster Session and revaccinated ani
Page 138 and 139:
Poster Session weeks after infectio
Page 140 and 141:
Poster Session melitensis wild type
Page 142 and 143:
Poster Session indicated that HS en
Page 144 and 145:
Poster Session This study aimed to
Page 146 and 147:
Poster Session definition. Reviewin
Page 148 and 149:
Poster Session ApaI+0/MseI+G) were
Page 150 and 151:
Poster Session 109- COMPARATIVE PRO
Page 152 and 153:
Poster Session enterobactin. At the
Page 154 and 155:
Poster Session apparent differences
Page 157 and 158:
Author index A Abalos, P.----------
Page 159 and 160:
Author index GonzálezCarreró, M I
Page 161 and 162:
Author index Q Qasem, J A.---------
Page 163 and 164:
List of participants ABERNETHY, DAR
Page 165 and 166:
List of participants CARNERO OJEA,
Page 167 and 168:
List of participants Fax: 301 319 9
Page 169 and 170:
List of participants Phone: 1 97 98
Page 171 and 172:
List of participants IOANNOU, IOANN
Page 173 and 174:
List of participants LÓPEZ-GOÑI,
Page 175 and 176:
List of participants NEUBAUER, HEIN
Page 177 and 178:
List of participants E-mail: rajash
Page 179 and 180:
List of participants SUAREZ-GUEMES,
Page 181:
Sponsors Brucellosis 2003 Internati
show all

Brucellosis 2003 proceedings - PHIDIAS

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?