Brucellosis 2003 proceedings - PHIDIAS

Short Oral Communications
Vaccines
dominated immune response in goats, as determined by immunoglobulin G isotype
analysis.
VO3- IMMUNE RESPONSE IN CALVES VACCINATED WITH DNA VECTOR
ENCODING Cu/Zn SOD PROTEIN OF Brucella abortus.
A. Oñate 1 , I. Guzmán 1 , A. González 1 , S. Céspedes 1 , C. Muñoz 1 , R. Rivers 1 , H. Folch 2 , and E.
Andrews 1 . (1) Laboratorio de Inmunología Molecular, Departamento de Microbiología, Facultad de
Ciencias Biológicas. Universidad de Concepción. Concepción, Chile. (2) Instituto de Inmunología.
Universidad Austral de Chile. Chile.
Brucella abortus is an intracellular facultative bacterium, able to proliferate in
the host phagocytic cells, which allows it to avoid extra cellular defence’s
mechanisms and remain as a chronic form. In Chile bovine cattle is currently being
vaccinated with B. abortus RB51 and it has a variable efficacy that does not assure a
total protection. DNA vaccines are a powerful method of immunization, that has
proved to elicit both humoral and cellular immune response. At the moment, new
vaccination alternatives have been developed, the so-called DNA vaccines. This
study was conducted to evaluate in calves the immunogenicity of a DNA vaccine
encoding Brucella abortus Cu/Zn superoxide dismutase (SOD), a vector of known
immunogenic capacity in mice (accepted Infection and Immunity 71: 2003). Cattle
were immunized with expression plasmids in eukaryote cells (pcDNA3.1), either
bearing or not bearing the insert for SOD protein gene (sodC). As a positive control,
calves were vaccinated with Brucella RB51. Intramuscular (i.m.) immunization with
pcDNA-SOD induced lymphocyte proliferation against SOD recombinant protein and
bacterial in similar form to animals immunized with RB51. Also, prevalence of
antigenic specific ?d T cells proliferation was observed with pcDNA/SOD vaccine. All
experimental heifers groups (vaccinated with pcDNA/SOD and bacterial) developed
low level of serum antibodies. Our results suggest that this newly made plasmid from
pcDNA-SOD is effective in inducing an immune response against brucellosis in
cattle.
This work was supported by grant FONDECYT 1010851, Chile.
VO4- EVALUATION OF Brucella abortus S19 VACCINE STRAINS USED IN INDIA
BY BACTERIOLOGICAL AND MOLECULAR TESTS AND VIRULENCE STUDIES
IN BALB/c MICE.
Falguni Mukherjee 1 , Jainendra Jain 1 , María Jesús Grilló 2 , José María Blasco 2 , Mrinalini Nair 3 . (1)
Research and Development, National Dairy Development Board, Anand 388 001, Gujarat, India. (2)
Unidad de Sanidad Animal, Servicio de Investigación Agroalimentaria, Gobierno de Aragón, Apartado
727, Zaragoza, Spain. (3) Biotechnology Program, Department of Microbiology and Biotechnology
Centre, MS University, Baroda 390 002, Gujarat, India.
Two Brucella abortus S19 commercial vaccine strains used for vaccination
campaigns against bovine brucellosis in India and two S19 strains used as a internal
reference, were studied by both microbiologic and molecular assays, and tested for
virulence in BALB/c mice. The four strains tested were identified as B. abortus biovar
1 and all had the characteristics of strain S19, according sensitivity to penicillin and i -
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