Vol 43 # 3 September 2011 - Kma.org.kw

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198 Characterization of Acrylamide Mediated Testicular Toxicity in Rat: Light and Electron ... September 2011 Fig. 1: Shows the effects of different doses of AA on rats’ body weight during the treatment period (day 1 - 5). There is a statistically significant decrease in body weight (H = p < 0.05) of the groups treated with 45 and 60 mg/kg AA. that varied in its severity along with lethargy and reduction in activity. None of these potential markers of toxicity were present in the other treatment groups. Effect of AA on rats' body weight During the treatment period (day 1 - 5) there was a statistically significant drop in body weight (p < 0.05) in the groups of rats treated with 45 and 60 mg/kg AA. (Fig.1). During the recovery period, (5 - 8 days) after cessation of AA treatment, the loss of body weight Fig. 2: Shows the effects of different doses of AA on rats’ body weight during the recovery period (day 5 - 8), after cessation of AA treatment. There is a statistically significant decrease in body weight at AA doses of 45 and 60 mg/kg, (H = p < 0.05) and (HH = p < 0.01) respectively, as compared to the control group. became less pronounced with slight weight gain occurring in some animals of all groups. However, there was still a statistically significant decrease in body weight at AA doses of 45 and 60 mg/kg, (p < 0.05) and (p < 0.01) respectively, as compared to the control group (Fig. 2). Effect of AA on testis / body weight ratio This study revealed a significant increase (p < 0.05) of the testis / body weight ratio in the group treated Fig. 3: Shows effects of different doses of AA on rats’ testis / body weight ratio at the end of the experiment. There is a significant increase ( H = p < 0.05) in testis weight in relation to the body weight of the rats in the group treated with 15 mg/kg AA. Moreover, a very significant increase (HH = p < 0.01) in testis / body weight ratio was detected in the two groups treated with 45 and 60 mg/kg. Fig: 4: Shows the effects of different doses of AA on caudal sperm count. There is significant reduction (H = p < 0.05) in sperm count of the group treated with 60 mg/kg AA. Other groups showed no difference in relation to control group.
September 2011 KUWAIT MEDICAL JOURNAL 199 Fig. 5: Shows caudal sperm morphology of (A) control rat with normal head (black head arrow) (B) rat treated with 5 mg/kg AA showing detached tail (black arrow) (C) rat treated with 5 mg/kg AA showing twisted middle piece (black arrow) (x 40). (D) rat treated with 15 mg/kg AA showing cleaved heads(black arrow) (x 100). (E) rat treated with 45 mg/kg AA showing cleaved heads (arrow) (x 40). (F) rat treated with 60 mg/kg AA showing typical tail intersegmentation (small arrows) (x 40). Smears stained with Wright stain. with 15 mg/kg AA. Moreover, a highly significant increase (p < 0.01) in testis / body weight ratio was detected in the two groups treated with 45 and 60 mg/ kg of AA (Fig. 3). Effect of AA on epididymal (cauda) sperm count AA induced a significant reduction (p < 0.05) of sperm count in the group treated with 60 mg/kg AA (Fig. 4). Other groups showed no difference when compared to control group. Effect of Acrylamide on epididymal (Caudal) sperm morphology Abnormal sperm shapes have been detected in all used doses of AA. However, marked increase in the number of head / tail-cleaved sperms was noted maximally in those rats treated with 45 and 60 mg/ kg AA (Fig. 5). Moreover, an abnormality named tail intersegmentation, that was not detected during previous studies on AA was markedly observed among these groups (Fig. 5F). Tail intersegmentation was also observed to a lesser extent among control rats. Effect of AA on histological appearance of rat testes and epididymis At a dose of 5 mg/kg AA, the testes showed disruptions of its appearance. Some germ cells appeared degenerated and many basal vacuolization was
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Vol 43 # 3 September 2011 - Kma.org.kw

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