Vol 43 # 3 September 2011 - Kma.org.kw

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200 Characterization of Acrylamide Mediated Testicular Toxicity in Rat: Light and Electron ... September 2011 Fig. 6: (A) Control rat testis showing multiple cross sections in seminiferous tubules and normal Leydig cell, (B) control rat epididymis showing apparent sperm reserve in its lumen (H) x 40, (C) Testis of rat treated with 5 mg/kg AA showing multiple vacuoles (v), sloughing of germinal epithelium into lumen x 20, (D) Epididymis of rat treated with 5 mg/kg AA, appears normal x 20, (E) Testis of rat treated with 15 mg/kg AA showing degeneration of spermatogonia, hypospermatogenesis, widening of intercellular spaces and reduction in the mature spermatozoa in the lumen x 20, (F) Epididymis of rat treated with 15 mg/kg AA, showing reduction in sperm reserve (H) x 40. Sections stained with H&E stain. observed between the inner cells of the seminiferous tubules. However, the epididymis appeared normal (Fig. 6, C and D). At a dose of 15 mg/kg AA, the testes showed increased debris, widening of the intercellular spaces and reduction of the mature spermatozoa in the lumen of the tubule. The epididymis appeared normal with good sperm reserve (Fig. 6, E and F). At a dose of 45 mg/kg AA, the testes showed sloughed seminiferous epithelium and multinucleated giant (MNG) cells in tubular lumen. Further, Leydig cells appeared atrophied while epididymis was apparently normal (Fig. 7 A, B and C). At the higher dose of AA (60 mg/kg), the histopathological changes observed were similar to those of the lower dose (45 mg/kg) but with a more pronounced degree. The MNG cells increased in number and size and some of them had cytoplasmic vacuolization. They appeared in both ST and epididymis. The mature spermatozoa were significantly reduced in the lumen of the tubules (Fig. 7, D, E and F)
September 2011 KUWAIT MEDICAL JOURNAL 201 Fig. 7: (A) Testis of rat treated with 45 mg/kg AA showing small multinucleated giant cells (MNG) in their lumen (arrow head) and atrophy of Leydig cells (arrow) x 20, (B) Testis of same group showing shedding of germinal epithelium in the lumen x 40, (C) Epididymis of same group appears normal x 20, (D) Testis of rat treated with 60 mg/kg AA showing multiple (MNG) cells in the lumen (small arrow head), scanty mature spermatozoa and atrophy of Leydig cells (arrow) x 20, (E) Testis of same group showing ST with multiple MNG (small arrow) with vacuolated cytoplasm, degenerated spermatogonia and reduction in mature spermatozoa (star) x 40, (F) Epididymis of same group showing multiple MNG (arrows) of different size in and clear reduction in sperm reserve x 40. Sections stained with H&E stain. Electron microscopy (EM) on rat testes The testes of rat treated with 45mg/kg AA showed degeneration of spermatogonia with large basal vacuolization, widening of intercellular junctions and degeneration of peritubular myoid cell (Fig. 8 C). While Sertoli cells of the control testis showed normal histological features (Fig. 8 B), those of the AA treated rat showed numerous histological abnormalities. These abnormalities included darkening of Sertoli cell nucleus, detachment of the cell from the basement membrane and increase in the number and size of lipid droplets in its cytoplasm. Further, failure of sperm release and phagocytosis of some sperms by Sertoli cell were also detected (Fig. 8 D). The transverse and longitudinal sections in the axoneme of developing spermatids from control rat appeared normal apart from dissolution of one circumferential rib in the principal piece of few spermatids (intersegmentation of fibrous sheath). (Fig. 9 C). As regards the AA treated group, the developing
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