Vol 43 # 3 September 2011 - Kma.org.kw

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Characterization of Acrylamide Mediated Testicular Toxicity in Rat: Light and Electron ...
September 2011
Fig. 6: (A) Control rat testis showing multiple cross sections in seminiferous tubules and normal Leydig cell, (B) control rat epididymis
showing apparent sperm reserve in its lumen (H) x 40, (C) Testis of rat treated with 5 mg/kg AA showing multiple vacuoles (v), sloughing
of germinal epithelium into lumen x 20, (D) Epididymis of rat treated with 5 mg/kg AA, appears normal x 20, (E) Testis of rat treated with
15 mg/kg AA showing degeneration of spermatogonia, hypospermatogenesis, widening of intercellular spaces and reduction in the mature
spermatozoa in the lumen x 20, (F) Epididymis of rat treated with 15 mg/kg AA, showing reduction in sperm reserve (H) x 40. Sections
stained with H&E stain.
observed between the inner cells of the seminiferous
tubules. However, the epididymis appeared normal
(Fig. 6, C and D). At a dose of 15 mg/kg AA, the testes
showed increased debris, widening of the intercellular
spaces and reduction of the mature spermatozoa in the
lumen of the tubule. The epididymis appeared normal
with good sperm reserve (Fig. 6, E and F).
At a dose of 45 mg/kg AA, the testes showed
sloughed seminiferous epithelium and multinucleated
giant (MNG) cells in tubular lumen. Further, Leydig
cells appeared atrophied while epididymis was
apparently normal (Fig. 7 A, B and C). At the higher
dose of AA (60 mg/kg), the histopathological changes
observed were similar to those of the lower dose
(45 mg/kg) but with a more pronounced degree.
The MNG cells increased in number and size and
some of them had cytoplasmic vacuolization. They
appeared in both ST and epididymis. The mature
spermatozoa were significantly reduced in the
lumen of the tubules (Fig. 7, D, E and F)