SCIENTIFIC REPORT 2004 - Sylvester Comprehensive Cancer Center

T U M O R I M M U N O L O G Y P R O G R A M
Another project in Dr. Blomberg’s laboratory
involves clinical research with breast cancer patients.
In collaboration with Michael H. Antoni,
Ph.D., in the department of Psychiatry and Behavioral
Sciences, Charles S. Carver, Ph.D., in the
department of Psychology, Sharlene Weiss, R.N.,
Ph.D., in the department of Medicine, and members
of the Cancer Prevention and Control Program
at the University of Miami Sylvester
Comprehensive Cancer Center, researchers are
measuring the status of various immune parameters
in patients in response to psychosocial intervention
(e.g., group therapy, stress reduction).
Preliminary experiments have shown that intervention
patients have an improved immune response
as seen by the ability of their T cells to
proliferate in response to an antigen-specific receptor
stimulus (anti-CD3). Current studies are
measuring T, NK, and lymphokine-activated
killer cells (LAK) cytotoxic function as well as
potential TH1/TH2 differences by cytokine production
resulting from T-cell stimulation. These
studies are important to allow optimal immune
response in cancer patients, which will better
detect and destroy residual cancer and allow for
better patient survival.
SELECTED PUBLICATIONS
2002
Jin, Y, Fuller, L, Carreno, M, Esquenazi, V,
Blomberg, BB , Wei, YT, Ciancio, G, Burke, GW
3rd, Tzakis, A, Ricordi, C, and Miller, J. Functional
and phenotypic properties of peripheral T
cells anergized by autologous CD3(+) depleted
bone marrow cells. Human Immunology 63:567-
75, 2002.
Burke, GW, Ciancio, C, Blomberg, BB , Rosen,
A, Suzart, K, Roth, D, Kupin, W, Esquenazi, V,
and Miller, J. Randomized trial of three different
immunosuppressive regimens to prevent chronic
renal allograft rejection. Transplantation Proceedings
34:1610-11, 2002.
Ricordi, C, Tzakis, A, and Miller, J. Human bone
marrow cells retrovirally transduced with the allogeneic
class II gene, HLA-DR3beta, down regulate
anti-allogeneic responses of autologous
lymphoid cells. Human Immunology 63:S19,
2002.
2003
Mathew, JM, Blomberg, BB, Fuller, L, Burke,
GW, Ciancio, G, Kenyon, N, Ricordi, C, Tzakis,
AG, Esquenazi, V, and Miller, J. A novel microcell-mediated
lympholytic assay for the evaluation
of regulatory cells in human alloreactive
CTL responses. Journal of Immunological Methods
272:67-80, 2003.
Blomberg, BB , Hussini, S, Fainman, H, Mathew,
JM, Hernandez, A, Carreno, M, Hnatyszyn, HJ,
Garcia-Morales, R, Fuller, L, Rosen, A, Ricordi,
C, Tzakis, A, Miller, J, and Esquenazi, V.
Retroviral transduction of an allogeneic class II
gene into human bone marrow down regulates
allo-immune reactivity. Human Immunology
64:S128, 2003.
Hernandez, A, Lindner, I, Blomberg, BB,
Hussini, S, Burger, M, Mathew, JM, Carreno, M,
Garcia-Morales, R, Fuller, L, Jin, Y, Rosen, A,
Lee, KP, Miller, J, and Esquenazi, V. Suppression
of allogeneic T-cell proliferation through blocking
of NF-κB in the differentiation process of human
dendritic cells. Human Immunology 64:S128,
2003.
Mathew, JM, Alvarez, S, Vallone, T, Blomberg,
BB, Joshua, M, and Esquenazi, V. A human-
SCID-mouse-islet transplant model for the evaluation
of the regulatory activity of donor bone
marrow cells. Human Immunology 64:S7, 2003.
Van Der Put, E, Sherwood, EM, Blomberg, BB,
and Riley, RL. Aged mice exhibit distinct B cell
precursor phenotypes differing in activation, proliferation,
and apoptosis. Experimental Gerontology
38:1137-47, 2003.
Blomberg, BB , Mathew, J, Fainman, H, Hussini,
S, Carreno, M, Hnatyszyn, H, Garcia-Morales,
R, Fuller, L, Vallone, T, Rosen, A, Esquenazi, V,
UM/Sylvester Comprehensive Cancer Center Scientific Report 2004 107