SCIENTIFIC REPORT 2004 - Sylvester Comprehensive Cancer Center

S H A R E D R E S O U R C E S
G E N E K N O C K O U T A N D T R A N S G E N E F A C I L I T Y
MANAGER
Thomas R. Malek, Ph.D.
Professor and Vice Chair of Microbiology
and Immunology
PURPOSE
The Gene Knockout and Transgene Facility
produces transgenic and knockout mice and
trains investigators to apply this technology to
their research. The major goal of the facility is to
provide this technological capability to peer-reviewed
funded cancer researchers at UM/
Sylvester.
SERVICES
This facility provides the following services to
cancer center members:
1) Set up breeding of donor and recipient mice
and subsequently check plugs to confirm
mating.
2) Perform vasectomies of male mice for the
recipient colony.
3) Collect fertilized eggs or blastocysts.
4) Sort and culture eggs.
5) Prepare microinjection needles.
6) Microinject eggs with DNA or blastocysts
with cells.
7) Inject donor and recipient mice with
hormones.
8) Perform microsurgery to re-implant eggs or
blastocysts.
9) Culture and transfer embryonic stem cells for
microinjection into blastocysts.
10) Generate gene-targeting constructs rapidly
by using an arrayed library.
11) Provide investigators with tissue biopsies to
screen for transmission of transgene.
12) Advise investigators in the production of
transgenic and gene knockout constructs.
13) Advise investigators in culturing and gene
targeting in embryonic stem cells.
150
UM/Sylvester Comprehensive Cancer Center Scientific Report 2004