SCIENTIFIC REPORT 2004 - Sylvester Comprehensive Cancer Center

More documents

Recommendations

Info

S H A R E D R E S O U R C E S M O L E C U L A R A N A L Y S I S C O R E MANAGER Roland Jurecic, Ph.D. Assistant Professor of Microbiology and Immunology PURPOSE The Molecular Analysis Core provides UM/Sylvester members with: 1) capillary-based DNA sequencing of plasmids and polymerase chain reaction (PCR) fragments, 2) shotgun sequencing of large cDNA and genomic DNA inserts (transgenic and knockout/targeting vectors), 3) basic DNA fragment analysis, and 4) real time quantitative PCR and reverse transcriptase-PCR (RT-PCR) using LightCycler technology. 3) Basic DNA fragment analysis. Detection of isoforms, alternative transcripts, and DNA rearrangements, etc. 4) Automated heterozygote detection. Quantitative Real Time PCR Techniques Using the Roche LightCycler 1) Real time quantitative PCR and RT-PCR. 2) Full melt curve analysis of amplified products. 3) Design and testing of fluorogenic probes for rapid and sensitive detection of gene targets. 4) Genotyping of transgenic and knockout mouse models. SERVICES This facility provides UM/Sylvester investigators, in support of their peer-reviewed research, with the following services: Genetic Analysis using the Beckman CEQ 2000 Genetic Analyzer 1) Capillary-based DNA sequencing of plasmids and PCR fragments (800 base pairs in 90 minutes), using standard (T3, T7, Sp6, M13, etc.) or custom-designed primers. 2) Shotgun sequencing of large cDNA and genomic DNA inserts (transgenic and knockout/targeting vectors) using transposon technology for fast and easy insertion of sequencing primer binding sites and kanamycin resistance markers into target DNA in vitro. Selection of different clones with transposon integrated only into the genomic insert (not the plasmid backbone) by digest restriction and size difference. Based on the insert size, 20 to 40 different clones are subjected to automated sequencing and assembled into contigs using sequence utility software. UM/Sylvester Comprehensive Cancer Center Scientific Report 2004 153
S H A R E D R E S O U R C E S P O P U L A T I O N R E S E A R C H C O R E (Developing Shared Resource) DIRECTOR Michael H. Antoni, Ph.D. Professor of Psychology MANAGER Dorothy F. Parker, M.H.S. PURPOSE The purpose of the Population Research Core will be to provide services to support population-based cancer prevention and control research at UM/Sylvester and to help increase the diversity of study participants to represent the racial, ethnic, and socioeconomic composition of South Florida’s diverse and unique community. The Population Research Core evolved from the Florida Comprehensive Cancer Control Initiative (FCCCI), a project funded by the Centers for Disease Control that established regional cancer control collaboratives throughout Florida. UM/Sylvester continues to support the Southeast Florida Regional Cancer Control Collaborative, a group of more than 40 organizations that are a potential source of recruitment for populationbased studies. FCCCI’s staff of three also supports the Population Research Core. They have experience in cancer control research, data analysis, and community outreach. SERVICES The Population Research Core will offer services in three areas: recruitment, data and measurement, and education. Additionally, in an effort to better meet the needs of UM/Sylvester investigators, the core is conducting a faculty survey to determine what is needed to facilitate research and recruitment. Recruitment 1) Help UM/Sylvester investigators develop and assess recruitment and retention plans for funded projects. 2) Increase awareness of population research studies open for accrual. 3) Serve as liaison with local health care providers and community organizations. 4) Develop agreements and procedures for recruitment of participants in cancer control studies. 5) Pretest and/or conduct focus groups with target populations to develop appropriate recruitment tools and strategies (e.g., brochures, flyers, and advertisements). Data and Measurement 1) Provide aggregate local, regional, and national cancer and demographic data. 2) Help develop or identify appropriate questionnaires and survey tools. 3) Arrange for translation of materials (e.g., into Spanish and Haitian Creole). 4) Provide assistance with data collection tools and database design. Education 1) Assess needs of investigators for understanding issues related to population science. 2) Provide workshops and training on issues such as: community outreach and campus-community research concerns, cultural and socioeconomic factors that affect recruitment strategies, study design, the informed consent process, and participation in studies. 154 UM/Sylvester Comprehensive Cancer Center Scientific Report 2004
Page 1 and 2:
S C I E N T I F I C R E P O R T 2 0
Page 3 and 4:
I N T R O D U C T I O N A N D P R O
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
L E A D E R S H I P MULTIDISCIPLINA
Page 13 and 14:
L E A D E R S H I P EXTERNAL ADVISO
Page 15 and 16:
C A N C E R P R E V E N T I O N A N
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
C L I N I C A L O N C O L O G Y R E
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
T U M O R C E L L B I O L O G Y P R
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116: T U M O R C E L L B I O L O G Y P R
Page 117 and 118: T U M O R I M M U N O L O G Y P R O
Page 143 and 144: V I R A L O N C O L O G Y P R O G R
Page 157 and 158: S H A R E D R E S O U R C E S 3) La
Page 159 and 160: S H A R E D R E S O U R C E S C E L
Page 161 and 162: S H A R E D R E S O U R C E S D N A
Page 163 and 164: S H A R E D R E S O U R C E S G E N
Page 165: S H A R E D R E S O U R C E S I N F
Page 169 and 170: P U B L I C A T I O N S 2 0 0 2 - 2
Page 205 and 206: G L O S S A R Y IFN—interferon IL
show all

SCIENTIFIC REPORT 2004 - Sylvester Comprehensive Cancer Center

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?