SCIENTIFIC REPORT 2004 - Sylvester Comprehensive Cancer Center

S H A R E D R E S O U R C E S
F L O W C Y T O M E T R Y R E S O U R C E
MANAGER
Richard L. Riley, Ph.D.
Professor of Microbiology and Immunology
PURPOSE
The Flow Cytometry Resource provides
UM/Sylvester investigators, in support of
their peer-reviewed funded research, with sophisticated
methods for analysis and preparative
sorting of normal and tumor cells, and trains
investigators in the use of flow cytometry for
their research.
SERVICES
This resource provides the following services to
cancer center members:
1) Laser-excited flow cytometry for analysis of
cell surface antigens expressed in complex cell
mixtures; up to four different fluorescent
parameters and two light-scatter parameters
(forward and side scatter) can be analyzed
simultaneously.
2) Laser-excited cell sorting (six parameters) for
isolation of selected cell populations from
heterogeneous mixtures.
3) DNA content/cell cycle analysis via both
visibly excited dyes (propidium iodide) and
UV-excited dyes (Hoescht dyes) with pulse
processing or doublet discrimination.
4) High-efficiency sorting and sorting of large
particles via the MacroSort system.
5) Intracellular calcium ratio measurements.
6) Applications for limiting dilution or cloning
experiments via the automatic cell deposition
unit (ACDU).
7) Training in the use of the FACScan and LSR
analytical flow cytometers, computer programs
for data analysis, and data storage; consultation
in the procedures for cell preparation,
staining, fixation, data analysis, and preparative
sorting also are provided.
UM/Sylvester Comprehensive Cancer Center Scientific Report 2004 149