Exhibit 8, 100416 Brazil FMD Risk Evaluation - R-Calf

assay test. Other diagnostic tests include one- or two-dimensional electrophoresis of the viral DNA,
isoelectric focusing of the viral structural proteins, or nucleotide sequencing of the viral RNA[47].
FMD virus is a relatively resilient virus. It can survive up to 15 weeks in feed, 4 weeks on cattle
hair, and up to 103 days in wastewater. The survival of the virus in animal tissues is closely
associated with the acidity of that tissue. For example, in muscular tissues the acidity of rigor
mortis, which occurs naturally, inactivates the virus. The production of lactic acid in these tissues
during maturation is considered the primary factor for inactivation [43]. An acid environment where
the pH is less than 6.0 will destroy the virus quickly [43, 59]. Several studies showed that in tissues
where no acidification occurs (e.g., lymph nodes, bone marrow, fat, and blood), the virus may
survive for extended times in cured, uncured, and frozen meat [43, 60, 61]. Heating at 50° C [43]
and up to 155° F [59] will inactivate the virus.
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