Basics of Light Microscopy Imaging - AOMF

More documents

Recommendations

Info

GIVE YOUR EYES TIME TO ADJUST TO THE HIGH LIGHT EFFICIENCY. OLYMPUS SZX2. If you’re used to other stereo microscopes, you’d better be prepared before you start working with the new Olympus SZX2 range. The peerless resolution and exceptional light efficiency enable you to see fluorescence with brightness and brilliance like never before, while providing optimum protection for your specimens at the same time. All this makes Olympus SZX2 stereo microscopes the perfect tools for precise fluorescence analysis on fixed and living samples. Importantly, the innovations present in the SZX2 series go far beyond fluorescence. The optimised 3-D effect offers superior conditions for specimen preparation and precise manipulation. The unique ultra-flat LED light base provides bright and even illumination of the entire field of view at a constant colour temperature. Moreover, the ComfortView eyepieces and new trinocular observation tubes make sure your work is as relaxed as it is successful. The future looks bright with Olympus. For more information, contact: Olympus Life and Material Science Europa GmbH Phone: +49 40 2 37 73 54 26 E-mail: microscopy@olympus-europa.com www.olympus-europa.com O l y m p u s M i c r o s c o p y
Introduction In this wide-ranging review, Olympus microscopy experts draw on their extensive knowledge and many years of experience to provide readers with a highly useful and rare overview of the combined fields of microscopy and imaging. By discussing both these fields together, this review aims to help clarify and summarise the key points of note in this story. Encompassing the creation of highly detailed specimen views through to final image capture and processing, the story will also be backed up by useful insights, examples, as well as handy hints and tips along the way. To make it easy to dip in and dip out of this comprehensive overview, the review is clearly structured into several chapters. These take the reader from explanations of the physics of light, colour and resolution, through details of contrast and fluorescence techniques, to finish up with discussion on 3D imaging. Highlighting and summarising key points made within this review are a series of information boxes. These also aim to provide useful insight on techniques, as well as expert advice on microscope set up. Content Editorials 2–3 Trust the Colours 4 The Resolving Power 12 Contrast and Microscopy 22 Shining Fluorescence Details 34 3D Imaging 42 Contact and Acknowledgement 52 Imprint 52 Basics of light Microscopy & Imaging •
Page 1: Basics of Light Microscopy Imaging
Page 5 and 6: “You Can Get Keywords for Expandi
Page 7 and 8: Light How do we describe light that
Page 9 and 10: Fig. 5a-c is showing the emission s
Page 11 and 12: Fig. 6: Schematic of axial chromati
Page 13 and 14: Fig. 11: White balance adjustment u
Page 15 and 16: Human vision For most of us, seeing
Page 17 and 18: the resolution which can be reached
Page 19 and 20: Fig. 16: Airy disk patterns of diff
Page 21 and 22: quisition. It is called deconvoluti
Page 23 and 24: Box 6: Extended Focal Imaging (efi)
Page 25 and 26: All cats are grey in the dark Why a
Page 27 and 28: Fig. 29: Light path for darkfield c
Page 29 and 30: pixels are grouped in the centre of
Page 31 and 32: (fig. 34). In the condenser, a spec
Page 33 and 34: Where there is light, there is also
Page 35 and 36: Fig. 42: Brightfield image of a dia
Page 37 and 38: A first introduction Most of the ne
Page 39 and 40: act amount needed. The amount of li
Page 41 and 42: Fig. 49: A climate chamber offers f
Page 43 and 44: dyes can be shown as three differen
Page 45 and 46: Reality is multi-dimensional What d
Page 47 and 48: Fig. 57: Left: Height map. Middle a
Page 49 and 50: Fig. 60: Ovariole from Drosophila m
Page 51 and 52: Box 16: The steps involved in 3-D r
Page 53 and 54:
Box 18: How is an elemental map of
show all

Basics of Light Microscopy Imaging - AOMF

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?