Cetrimide Broth

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Products Media MacConkey with MUG MacConkey with MUG Used in the presumptive phase for the presence of coliform bacteria in water and foods. Selective to the detection of gram-negative lactose fermenting bacilli. This medium largely complies with the European Pharmacopeia. Description: MacConkey Broth was originally developed by MacCONKEY and HILL (1901) and the formulation for the agar was modified by MacConkey in 1950. MacConkey developed this medium for the cultivation of enteric pathogens and coliforms. It contains bile salts, which inhibit certain gram-negative bacteria, and crystal violet, which inhibits grampositive organisms such as enterococci and staphylococci. Combination of the lactose and neutral red indicator to produce pink to red colored colonies indicates an isolate with the ability to ferment lactose. Non-lactose fermenting organisms are colorless. The addition of MUG (4-Methylumbellifryl-β-Dglucuronide), which is a fluorogenic enzyme, allows the media to selectively identify Escherichia coli. MUG is hydrolyzed by the Escherichia coli specific enzyme β-glucuronidase to release 4-Methylumbellifone which fluoresces under ultraviolet light. Interpretation: Fluorescence under UV light is specific for the presence of Escherichia coli. Lactose fermenting organisms grow as pink to red colonies. Organisms Characteristics Coloring E. faecalis ATCC 29212 inhibited S. typhimurium ATCC 14028 Growth Clear E. coli ATCC 25922 Growth/fluorescence Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, incubated at 35º C for 24 hours. Checked for florescence at 366 nm. Negative control: Not undertaken. Sterility. 7 days plated sterility test. MacConkey with MUG: Escherichia coli ATCC 25922 forms as lactose-fermenting organisms pink to red colonies. Formulation: Make to 1 liter and adjust pH to 7.1 Casein Peptone 17.0 g Proteose Peptone 3.0 g Lactose 10.0g Bile Salts #3 1.5 g Sodium Chloride 5.0 g Neutral Red 30 mg Crystal Violet 0.1 mg MUG (4-Methylumbelliferylβ-D-glucuronide) 0.1 g Additional information: MacConkey Broth is a modification of the original bile salt broth where bromo cresol purple is used in place of neutral red or litmus as the indicator. Bile salts replace 0.5 % sodium taurocholate in the origin formulation. The addition of MUG (4-Methylumbellifryl-(-D-glucuronide), which is a fluorogenic enzyme, allows the media to selectively identify Escherichia coli. MUG is hydrolyzed by the Escherichia coli specific enzyme (β-glucuronidase to release 4-Methylumbellifone which fluoresces under ultraviolet light). Historical background: MacConkey Broth is a modification of the formula given by MacConkey which corresponds to the alternative formulation recommended by the World Health Organization. The medium is used for the presumptive determination of the presence of coliform organisms (gram-negative, lactose – fermenting bacilli) in water and milk as well as other materials. Presence of lactose – fermenting organisms is detected by the change of color of the medium (from purple to yellow) after inoculation and incubation.The original formula of MacConkey called for use of litmus as an indicator of acid reaction. In later investigations neutral red was found to be a more suitable indicator. More recently, Childe and Allen demonstrated an inhibitory effect on the growth of Escherichia coli by neutral red in this medium. Bromcresol purple is not only less inhibitory, but also gives a more clear – cut indication of acid reaction. Order information MacConkey with MUG Product Description Qty/Pkg Order No Ampouled Media 2 ml 50 10 496 118 Petri dishes with sterile pads 47 mm 100 10 498 544 Petri dishes with sterile pads 50 mm 50 10 445 905 * available on request 47
Media Mannitol Salt Broth Products Mannitol Salt Broth Used for the selective isolation and enumeration of staphylococci. In addition the medium complies with the recommendations in the United States Pharmacopeia. Description: Because of the amount of peptones and beef extract, Mannitol Salt is a nutrient rich medium. Most bacteria (other than staphylococci) are inhibited by the high concentration of sodium chloride. Organisms capable of fermenting mannitol e.g. Staphylococcus aureus, cause a pH change in the media. With phenol red as the pH indicator the colonies appear with a yellow coloration. Interpretation: Typical pathogenic staphylococci ferment mannitol and form yellow colonies with yellow zones, while typical non-pathogenic staphylococci do not ferment mannitol and form red colonies. Quality Control and recommended incubation conditions: Positive control: Staphylococcus aureus ATCC 25923, 24–48 hours at 35º C. Negative control: Escherichia coli ATCC 25922, 24–48 hours at 35º C. Sterility: 7 days plated sterility test. Appearance of Colonies Surrounded by bright yellow zones, abundant growth pink to red colonies growth is usually poorer Microorganisms Mannitol-positive: S. aureus Mannitol-negative: S. epidermis and others Mannitol Salt Broth: Staphylococcus aureus ATCC 25923 forms typical yellow colonies with zones on this media. Indication for Mannitol positive organisms. Formulation: Per liter of water adjusted to pH 7.4 ± 0.2 Beef Extract Pancreatic Digest of Casein Peptic Digest of Animal Tissue Sodium Chloride d-Mannitol Phenol Red 1.0 g 5.0 g 5.0 g 75.0 g 10.0 g 25 mg Organisms Characteristics Coloring S. aureus ATCC 25923 Growth Yellow with yellow zones S. epidermidis ATCC 12228 Growth Red with no zone E. coli ATCC 25922 inhibited E. aerogenes ATCC 13048 inhibited Historical background: The tolerance of Staphylococcus aureus to high concentrations of sodium chloride was reported by Koch in 1942. In 1945, Chapman described a formulation which incorporated 7.5 % sodium chloride in phenol red mannitol agar to successfully cultivate pathogenic staphylococci. These coagulase positive organisms grew in large colonies surrounded by yellow zones. Non-pathogenic staphylococci are usually less luxuriant after the 36 hour incubation period recommended by Chapman. Mannitol Salt Broth and Agar is recommended for the enumeration of staphylococci in food and dairy products by the American Public Health Association. Order information Mannitol Salt Media Product Description Qty/Pkg Order No Ampouled Media 2 ml 50 10 496 121 Petri dishes with sterile pads 47 mm 100 10 498 544 Petri dishes with sterile pads 50 mm 50 10 445 905 * available on request 48
Page 1 and 2: Selective enrichment of following m
Page 3 and 4: Media Brilliant Green Bile Broth 2
Page 5 and 6: Media EC Broth Products Organisms G
Page 7 and 8: Media Enterococcus Broth Products E
Page 9 and 10: Media HPC Broth with TTC Products H
Page 11: Media Lauryl Sulfate or Lauryl Tryp
Page 15 and 16: Media M-Endo Coliform Broth Product
Page 17 and 18: Media M-FC with Rosolic Acid Produc
Page 19 and 20: Media M-Green Yeast and Mold Produc
Page 21 and 22: Media MRS Broth Products MRS Broth
Page 23 and 24: Media Orange Serum Media Products O
Page 25 and 26: Media Pseudomonas Broth Products Ps
Page 27 and 28: Media Sabouraud Dextrose Broth Prod
Page 29 and 30: Media Standard Methods Agar Product
Page 31 and 32: Media Trypticase Soy Broth (TSB) Pr
Page 33 and 34: Media Trypticase Soy Broth - Double
Page 35: Media Wallerstein Nutrient Broth (W

Cetrimide Broth

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