Cetrimide Broth

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Products Media MI Broth and MI Agar MI Broth and MI Agar Used for the simultaneous detection of total coliforms and Escherichia coli in water according to the Surface Water Treatment Rule (USEPA) and the Total Coliform Rule (USEPA) Description: MI Broth detects the presence of coliform bacteria by the production of β-galactosidase, which cleaves the substrate MUGal to produce 4-Methylumbelliferone, which fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. Escherichia coli is detected by the compound IBDG. The β-glucuronidase produced by Escherichia coli cleaves the substrate to produce a blue indigo color in the colonies. As Escherichia coli is also a total coliform, and also produces β-galactosidase it will also fluoresce. The antibiotic cefsulodin is added to inhibit the growth of gram-positive bacteria and some non-coliform gramnegative bacteria that can cause false positive reactions. MIBlue is specially developed for the food industry. Interpretation: Fluorescent blue colonies are Escherichia coli. Colonies that demonstrate blue/white fluorescence where the colonies are clear, cream or pale yellow in color are other coliform organisms. Clear colonies that do not fluoresce are noncoliforms. Organisms Characteristics Coloring E. coli ATCC 25922 Growth Blue with fluorescence E. aerogenes ATCC 13048 Growth Yellow with fluorescence P. aeruginosa ATCC 10145 Complete inhibition Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, 18–24 hours at 35º C. Enterobacter aerogenes ATCC 13048, 18–24 hours at 35º C. Negative control: Pseudomonas aeruginosa ATCC 10145, 24 hours at 35º C. Sterility: 7 days plated sterility test. MI-Media: Pure Culture of Escherichia coli ATCC 25922 with UV light. Formulation: Per liter of Water adjusted to pH 6.95 ± 0.2 Protease peptone Yeast extract β-d-lactose MUGal NaCl K2HPO4 KH2PO4 Sodium lauryl sulfate Sodium desoxycholate IBDG Cefsulodin (Agar) 5.0 g 3.0 g 1.0 g 0.1 g 7.5 g 3.3 g 1.0 g 0.2 g 0.1 g 0.32 g 5 mg 15.0 g Order information MI Media MI-Broth Media: Mixed Culture with Escherichia coli ATCC 25922 and Enterobacter aerogenes ATCC 13048 without UV. Product Description Qty/Pkg Order No Ampouled Media 2 ml 50 10 496 191 Bottled broth 50 ml 1 10 496 851 Bottled agar 50 ml 1 10 496 847 Petri dishes with sterile pads 47 mm 100 10 498 544 Petri dishes with sterile pads 50 mm 50 10 445 905 MIBlue 10 496 501* * available on request 55
Media MRS Broth Products MRS Broth Used for the isolation and cultivation of lactobacilli. MRS Broth complies with the German DIN-Norm 10109 and the International Standard ISO 13721 for the detection of lactosein meat and to the regulations acc. to § 35 LMBG (06.00/35) for the detection in meat. Description: MRS medium supports luxuriant growth of all lactobacilli, even the slow growing species. Interpretation: Lactobaccili appear as white colonies. Pediococcus and Leuconostoc may also grow on MRS. Quality Control and recommended incubation conditions: Positive control: Lactobacillus plantarum ATCC 8014 Lactobacillus fermentum ATCC 9338 incubated at 35º C for 48–72 hours. Negative control: Not undertaken. Sterility: 7 days plated sterility test. MRS Media; Pure Culture of Lactobacillus plantarum ATCC 8014. Formulation: Per liter of water adjusted to pH 6.2 ± 0.2 Pancreatic Digest of Casein Beef extract Yeast extract Dextrose Dipotassium Phosphate Polysorbate 80 Ammonium citrate Sodium acetate Magnesium sulfate Manganese sulfate 10.0 g 8.0 g 4.0 g 20.0 g 2.0 g 1.0 g 2.0 g 5.0 g 0.2 g 50 mg Additional information: The media contains special growth factors for lactobacilli like polysorbate, acetate, magnesium and manganese. These compounds are known as rich nutrient base. On a very low degree of selectivity, Pediococcus and Leuconostoc species and other secondary bacteria can be cultivated. Historical background: MRS Agar was developed by de Man, Rogosa & Sharper for the cultivation of Lactobacillus species. The medium will effectively support the growth of many strains of lactobacilli that do not generally grow as well as other media designed for this purpose. Due to the nutritional factors and the neutral pH other organisms which are not fastidious will grow on the medium. Lactobacillus will grow well on the surface of the medium as well as in deep culture preparations. Although enrichment with carbon dioxide is unnecessary with this medium, the atmosphere must be kept fairly moist Order information MRS Media Product Description Qty/Pkg Order No Ampouled Media 2 ml 50 10 496 112 Bottled broth 9 ml 20 10 496 737* Petri dishes with sterile pads 47 mm 100 10 498 544 Petri dishes with sterile pads 50 mm 50 10 445 905 * available on request 56
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Cetrimide Broth

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