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Media<br />
EC <strong>Broth</strong><br />
Products<br />
Organisms<br />
Growth at 44.5º C<br />
E. coli ATCC 25922 Growth / gas<br />
E. aerogenes ATCC 13048 Growth / no gas<br />
E. faecalis ATCC 29212 inhibited<br />
Organisms<br />
Growth at 37º C<br />
E. coli ATCC 25922 Growth / gas<br />
E. aerogenes ATCC 13048 Growth / gas<br />
E. faecalis ATCC 29212 inhibited<br />
Historical background:<br />
EC <strong>Broth</strong> was developed by Hajna and Perry for use in the detection of<br />
coliform bacteria at 37° C and Escherichia coli at 44.5° C. This buffered<br />
lactose broth was designed to improve the methods of detection of<br />
contamination of waters, milk and shellfish. Bile salts were incorporated to<br />
inhibit the growth of gram-positive cocci and sporeformers which<br />
frequently were responsible for false-positive results obtained when using<br />
lactose broth or lauryl tryptose broth. The EC <strong>Broth</strong> formula conforms to<br />
that recommended by the APHA for use in determining the source of water<br />
pollution. Through employment of the elevated temperature confirmatory<br />
test procedure, differentiation can be made between coliforms of fecal<br />
origin (intestine of warm-blooded animals) and coliforms from other<br />
sources<br />
Order information EC Media<br />
Product Description Qty/Pkg Order No<br />
Bottled broth 9 ml, vials, Durham tubes 20 10 496 714<br />
40
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