Cetrimide Broth

Media
Pseudomonas Broth
Products
Pseudomonas Broth
Used for the isolation of Pseudomonas
and differentiating Pseudomonas
aeruginosa from other pseudomonads
based on pigment formation.
Pseudomonas Broth complies with
the formulation recommendations of
the United States Pharmacopeia and
to the specifications in the DIN Norm
38411 (examination of water).
Description:
Pseudomonas aeruginosa is characterised
by the production of pyocyanin (a
blue green, water soluble, nonflourescent,
phenazine pigment), which is
stimulated by the inclusion of magnesium
chloride and potassium sulfate in
the broth. Irgasan, an antimicrobial
agent, selectively inhibits gram positive
and gram negative bacteria other than
pseudomonads. Glycerol serves as both
an energy source and helps in the
promotion of pyocyanin.
Interpretation:
Development of a green to blue green
pigmentation surrounding colonies is
positive for Pseudomonas aeruginosa.
Other pseudomonads develop as clear
to amber yellow colonies. Non-pseudomonads
are suppressed.
Organisms
Characteristics Coloring
P. aeroginosa ATCC 10145 Growth Blue to
blue-green
P. aeroginosa ATCC 27853 Growth Blue to
blue-green
E. coli ATCC 25922 inhibited
Quality Control and recommended
incubation conditions:
Positive control:
Pseudomonas aeruginosa ATCC 10145,
24–48 hours at 35º C.
Negative control:
Escherichia coli ATCC 25922,
24 hours at 35º C.
Sterility:
7 days plated sterility test.
Pseudomonas Media: Typical growth of Pseudomonas
aeroginosa ATCC 10145.
Formulation:
Per liter of Water adjusted
to pH 7.0 ± 0.2
Pancreatic Digest of Casein
Magnesium chloride
Potassium sulfate
Irgasan
Glycerol
20.0 g
1.4 g
10.0 g
0.25 g
20.0 ml
Additional information:
Identification of most Pseudomonas strains can be obtained by their
different pigmentation according to the compounds used in the media.
Some of the strains can only synthesize pyocyanin, some form only
fluorescein and others form both pigments.
Pseudomonas aeruginosa ATCC 27853 is characterised by the production
of pyocyanin (a blue green, water soluble, nonflourescent, phenazine
pigment), which is stimulated by the inclusion of magnesium chloride and
potassium sulfate in the broth. The same Pseudomonas aeruginosa strain
appears on Pseudomonas Broth as colonies surrounded by a yellow to
greenish-yellow zone when different types of peptone are added and
magnesium chloride and potassium sulphate are omitted.
BLAZEVIC et al. (1973), noted that some Pseudomonas aeruginosa strains
atypically appear as pyocyanin-negative, fluorescein-positive and for that
reason it is possible to differentiate them from Pseudomonas fluorescens
and Pseudomonas putida. Furthermore BRODSKY and NIXON (1973)
showed that cultivation on MacCONKEY agar can be used for a simple and
rapid differentiation of these strains.
Order information Pseudomonas Media
Product Description Qty/Pkg Order No
Ampouled Media 2 ml 50 10 496 119
Bottled broth 50 ml 8 10 496 775*
100 ml 1 10 496 776*
500 ml 1 10 496 777*
Bottled agar 50 ml 8 10 496 772*
100 m 1 10 496 773*
500 ml 1 10 496 774*
Petri dishes with sterile pads 47 mm 100 10 498 544
Petri dishes with sterile pads 50 mm 50 10 445 905
* available on request
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