Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Media<br />
HPC <strong>Broth</strong> with TTC<br />
Products<br />
HPC <strong>Broth</strong> with TTC<br />
Used for heterotrophic plate counts<br />
in the examination, for example, of<br />
potable water, dairy products and<br />
swimming pools.<br />
Description:<br />
HPC is used to determine total count at<br />
incubation temperatures of 35º C. All<br />
bacteria develop on HPC with indicator<br />
media and produce a red color as a<br />
result of the precipitation of formazan<br />
following the reduction of 2,3,5-<br />
triphenyltetrazolium chloride (TTC) by<br />
bacteria.<br />
Quality Control and recommended<br />
incubation conditions:<br />
Positive control:<br />
Escherichia coli ATCC 25922,<br />
incubate for 24–48 hours at 35º C.<br />
Negative control:<br />
Not undertaken.<br />
Sterility:<br />
14 days plated sterility test.<br />
HPC with Indicator: Escherichia coli ATCC 25922<br />
shows a typical red to pink coloring.<br />
Formulation:<br />
Per liter of water adjusted<br />
to pH 7.1 ± 0.2<br />
Interpretation:<br />
Colonies that develop on HPC are<br />
counted as total heterotrophic counts.<br />
Specific colony identification should be<br />
done using standard microbiological<br />
identification techniques.<br />
Peptone<br />
Gelatin<br />
Glycerol<br />
TTC, 1% solution<br />
30.0 g<br />
15.0 g<br />
15.0 ml<br />
10.0 ml<br />
Organisms<br />
Characteristics Coloring<br />
E. coli ATCC 25922 Growth Pink to red<br />
E. faecalis ATCC 29212 Growth Pink to red<br />
S. aureus ATCC 25923 Growth Pink to red<br />
Order information HPC with TTC<br />
Product Description Qty/Pkg Order No<br />
Ampouled Media 2 ml 50 10 496 151<br />
Petri dishes with sterile pads 47 mm 100 10 498 544<br />
Petri dishes with sterile pads 50 mm 50 10 445 905<br />
* available on request<br />
44
Change language