Cetrimide Broth

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Products Media Membrane Lauryl Sulfate Broth Membrane Lauryl Sulfate Broth For the presumptive identification of coliforms and Escherichia coli in water and drinking water. Prepared to the formula published in Journal of Hygiene (PHLS/SCA). Description: This media was developed for the detection of coliform organisms and is now the media of choice for the enumeration of total coliforms and Escherichia coli in the United Kingdom. This media replaced membrane enriched broth containing 0.4 % Teepol 610. To use membrane lauryl sulphate broth for the identification of thermotolerant coliforms incubate at 30º C for 4 hours then incubate for further 14 hours at 44º C. Yellow colonies are counted as presumptive coliforms which require confirmation. Organisms Characteristics Coloring 37 °C E. coli ATCC 25922 Growth Yellow E. aerogenes ATCC 13048 Growth Yellow E. faecalis ATCC 29212 Growth Pink or colorless This picture shows a mix culture on Membrane Lauryl Sulphate Broth incubated at 37° C. Organisms like Escherichia coli ATCC 25922 and Enterobacter aerogenes ATCC 13048 form yellow colonies whereas Enterococcus faecalis ATCC 29212 appears as pink colonies. Interpretation: Lactose acts as a source of fermentable carbohydrates for coliforms. Phenol red acts as an indicator of acidity as a result of coliform metabolism. Incubate for 4 hours at 30º C then increase the temperature to 37º C and incubate for further 14 hours. Yellow colonies are counted as presumptive coliforms which require confirmation. Pink or colorless colonies are not counted as coliforms. Organisms Characteristics Coloring 44 °C E. coli ATCC 25922 Growth Yellow E. aerogenes ATCC 13048 Inhibited Inhibited E. faecalis ATCC 29212 Inhibited Inhibited Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922 incubation for 4 hours at 30º C then for 14 hours at 37º C. Formulation: Per liter of water adjusted to pH 7.4 ± 0.2 Peptone Yeast extract Lactose Phenol red (0.4% solution) Sodium lauryl sulfate 40.0 g 6.0 g 30.0 g 50.0 ml 1.0 g Negative control: Not undertaken. Sterility: 7 days plated sterility test. Order information Membrane Lauryl Sulfate Media Product Description Qty/Pkg Order No Ampouled Media 2 ml 50 10 496 187 Petri dishes with sterile pads 47 mm 100 10 498 544 Petri dishes with sterile pads 50 mm 50 10 445 905 * available on request 49
Media M-Endo Coliform Broth Products M-Endo Coliform Broth M-Endo Broth is used for the enumeration of coliforms by membrane filtration. It is used to differentiate lactose from non-lactose fermenting intestinal organisms and as a presumptive test for coliforms. Description: M-Endo is a red colored media, which needs to be stored in the dark to prevent discoloration of the media. Gram-positive bacteria are inhibited on this media by the desoxycholate and lauryl sulfate. The addition of ethanol increases the antibacterial nature of the formulation. Lactose fermenting organisms form aldehydes, which react with Schiffs reagent (basic fuchsin and sodium sulfite) to give red colored zones around the colonies. Coliform colonies are therefore red with a characteristic metallic sheen. Interpretation: Production of both acid and aldehyde by lactose fermenters, such as Escherichia coli, produce deep red colonies that color the surrounding medium and have a green metallic sheen. Non-lactose fermenters form colorless, translucent colonies. Organisms Characteristics Coloring E. coli ATCC 25922 Growth Red with green metallic sheen E. aerogenes ATCC 13048 Growth Red colonies with or without green metallic sheen P. aeruginosa ATCC 10145 Growth Pink to colorless S. aureus ATCC 25923 Marked to complete inhibition Quality Control and recommended incubation conditions: Positive control: Escherichia coli ATCC 25922, 24 hours at 35º C. Negative control: Pseudomonas aeruginosa ATCC 10145, 24 hours at 35º C. Sterility: 7 days plated sterility test. M-Endo Broth: Mixed culture of Escherichia coli ATCC 25922: (red colonies with green metallic sheen) and Pseudomonas aeruginosa ATCC 10145 (pink to colorless). Formulation: Per liter of water adjusted to pH 7.2 ± 0.2 Peptones 10.0 g (equal parts digests of animal tissue and casein) Peptic Digest of Animal Tissue 5.0 g Pancreatic Digest of Casein 5.0 g Yeast extract 1.5 g Lactose 12.5 g Sodium chloride 5.0 g Dipotassium phosphate 4.375 g Monopotassium phosphate 1.375 g Sodium lauryl sulfate 50 mg Sodium desoxycholate 0.1 g Sodium sulfite 2.1 g Basic fuchsin 1.05 g 95 % Ethanol 30.0 ml Order information M-Endo Product Description Qty/Pkg Order No Ampouled Media 2 ml 50 10 496 103 Bottled broth 50 ml, Screw cap 8 10 496 700 50 ml, Septa cap 8 10 496 701 Petri dishes with sterile pads 47 mm 100 10 498 544 Petri dishes with sterile pads 50 mm 50 10 445 905 * available on request 50
Page 1 and 2: Selective enrichment of following m
Page 3 and 4: Media Brilliant Green Bile Broth 2
Page 5 and 6: Media EC Broth Products Organisms G
Page 7 and 8: Media Enterococcus Broth Products E
Page 9 and 10: Media HPC Broth with TTC Products H
Page 11 and 12: Media Lauryl Sulfate or Lauryl Tryp
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Cetrimide Broth

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