Cetrimide Broth

Media
Orange Serum Media
Products
Orange Serum Media
Used for the isolation and enumeration
of organisms associated with
the spoilage of citrus juices.
Description:
Organisms known to grow in single
strength and concentrated juices are
lactic acid and acetic acid bacteria and
yeast. Lactobacilli, Leuconostoc and
yeast have all been identified as spoilage
organisms by numerous authors.
Orange serum at pH 5.4 to 5.6 has
been reported to yield maximum
counts of all types of spoilage
organisms in mixed cultures, and in
single culture comparison tests.
Quality Control and recommended
incubation conditions:
Positive control:
Lactobacillus fermentum ATCC 9338,
48 hours at 35º C.
Candida albicans ATCC 10231,
48 hours at 25–30º C.
Negative control:
Not undertaken.
Sterility:
7 days plated sterility test.
Orange Serum Media agar is especially selective
for microorganisms which prefer low pH conditions
e.g. Lactobacillaceae and some yeast e.g.
Candida.
Formulation:
Per liter of water, adjusted
to pH 5.6 ± 0.2
Interpretation:
The low pH of the test solution usually
prohibits the development of other
microorganisms which cannot survive
low pH conditions. Therefore developing
colonies are presumed to be
problematic organisms.
Orange serum
Yeast extract
Tryptone
Dextrose
Dipotassium phosphate
10.0 g
3.0 g
10.0 g
4.0 g
2.5 g
Order information Orange Serum
Product Description Qty/Pkg Order No
Ampouled Media 2 ml 50 10 496 104
Bottled Agar 100 ml 1 10 496 713
Petri dishes with sterile pads 47 mm 100 10 498 544
Petri dishes with sterile pads 50 mm 50 10 445 905
* available on request
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